RNase activity in a convenient and sensitive colormetric assay that delivers results in real time. Great for Quality Testing for RNase contamination of materials and supplies.
Attogene has developed the first of its kind and patent pending technology that uses a novel RNA substrate tagged on the 5′ and 3′ end with a Biotin that is attached to our streptavidin colloidal gold reporter molecules and a test line tag. In the absence of RNases, the gold tagged RNA molecule will flow up the lateral flow strip and bind to the test line using an anti tag antibody (INDICATING that NO RNase is detected). When RNases are present, however, the RNA substrate is cleaved, and the 5′ and 3′ tags are spatially separated in solution cauing the test line to disapear.
This test can be used to rapidly and efficiently detect ribonucleases in both liquid and on solid surfaces and a perfect tool for monitoring mRNA vaccine manufacturing.
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IVD3020B MagPure Universal RNA Kit B
Product Info
Document
Product Info
Introduction
This product is suitable for rapid extraction of RNA from low RNA yield somples such as tissue (＜10mg), cells, bone marrow, fresh blood, and other clinical samples. RNA can be used directly for RT-PCR, Real time PCR, NGS, Viral RNA detection and so on.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from tissue, cell, whole blood
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Sample type
Tissues, cells, lymphocytes and other clinical sample
Sample amount
Cells grown in suspension: ≤3 x 106Animal tissue: ≤10mgPlant tissue: ≤30mgWhole blood: 0.5~1.0 ml fresh blood or bone marrow and fresh blood mixture
Kit Contents
Contents
IVD3020B-96
IVD3020B
Purification Times
96 Preps
200 Preps
MagPure Particles N
2.5 ml
5 ml
DNase I
2 x 600 µl
4 x 600 µl
DNase Buffer C
60 ml
120 ml
Buffer RLC
60 ml
120 ml
Buffer MCB*
60 ml
150 ml
Buffer GW1*
44 ml
110 ml
Buffer MW2*
50 ml
100 ml
RNase Free Water
20 ml
60 ml
Storage and Stability
DNase I should be shipped with ice pack and stored at -20°C after arrival. MagPure Particles N should be stored at 2–8°C for long time storage. The remaining kit components can be stored at room temperature(15–25°C) for up to18 months under these conditions.
Document
This product is suitable for rapid extraction of RNA from low RNA yield somples such as tissue (＜10mg), cells, bone marrow, fresh blood, and other clinical samples. RNA can be used directly for RT-PCR, Real time PCR, NGS, Viral RNA detection and so on.
1.Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.
2.Imported compressors fluorine free,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control.
3.Microprocessor control, digital display which indicates the temperature, speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.
4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection.
