RNase activity in a convenient and sensitive colormetric assay that delivers results in real time. Great for Quality Testing for RNase contamination of materials and supplies.
Attogene has developed the first of its kind and patent pending technology that uses a novel RNA substrate tagged on the 5′ and 3′ end with a Biotin that is attached to our streptavidin colloidal gold reporter molecules and a test line tag. In the absence of RNases, the gold tagged RNA molecule will flow up the lateral flow strip and bind to the test line using an anti tag antibody (INDICATING that NO RNase is detected). When RNases are present, however, the RNA substrate is cleaved, and the 5′ and 3′ tags are spatially separated in solution cauing the test line to disapear.
This test can be used to rapidly and efficiently detect ribonucleases in both liquid and on solid surfaces and a perfect tool for monitoring mRNA vaccine manufacturing.
Norgen’s EXTRAClean Urine Cell-Free Circulating RNA Purification Kits provide a fast, reliable, reproducible, and simple procedure for isolating circulating RNA and exosomal RNA from various urine inputs ranging from 250 μL and up to 30 mL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extensions, and expression array assays.
EXTRAClean Cell-Free Circulating RNA Purification Mini Kit: For sample volumes ranging from 250 μL to 2 mL
EXTRAClean Cell-Free Circulating RNA Purification Midi Kit: For sample volumes ranging from 2mL to 10mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL
EXTRAClean Cell-Free Circulating RNA Purification Maxi Kit: For sample volumes ranging from 10 mL to 30 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL.
All sizes, including miRNA and small RNA (< 200 nt)
Average Yields ¥
Variable depending on specimen
¥Please check page 6 of the protocol for average urine yields and common RNA quantification methods.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Collect from nasal, buccal, saliva, fecal, skin, surfaces, and more
Completely stabilize microbiota profiles from the point-of-collection
Minimize bias in data analysis: NGS (Metagenomics/Microbiome), PCR and microarrays
No need to immediately process samples: ship and store at ambient temperatures
DNA preservation at room temperature over 2 years
Renders sample non-infectious for safe and easy shipping
Compatible with DNA isolation kits and protocols: Optimized isolation procedure using Norgen’s Microbiome DNA Isolation Kit
Norgen’s Swab Collection and DNA Preservation System is designed for collection, ambient storage and transport of DNA from samples collected using a swab, including nasal, buccal, saliva, fecal, skin, surfaces, etc. The Swab Collection and DNA Preservation System contains Norgen’s Swab Preservative in a liquid format. The user simply collects the specimen using the provided swab, and then transfers the swab into the Swab Preservative. The Swab Preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Swab Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Swab Preservative allow DNA samples to be stored at room temperature for over 2 years.
DNA Isolation from Preservative
Swab samples collected and preserved using Norgen’s Swab Collection and DNA Preservation System are compatible with most DNA isolation methods. Samples stored in the tubes have been used successfully with most of Norgen Biotek’s DNA isolation kits and reagents. An optimized isolation procedure is available as part of Norgen’s Microbiome DNA Isolation Kit (Cat. 64100).
When stored at room temperature, unused Norgen Swab Preservative Tubes are stable through to the collect-before date without any reduction in performance. Please see the device insert or tube label for collect-before date.
[CD1021] SMOChem™ Deoxynucleotide (dNTP) Mix, 25 mM each (100 mM total), 500 µl x 6
Product Info
Document
Product Info
Description
The SMOChem™ Deoxynucleotide (dNTP) Mix is an aqueous solution that contains an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a concentration of 25 mM at pH 8.5. The dNTP Mix is designed for many different molecular biology applications that involved in DNA synthesis or labeling, such as PCR, real-time PCR, DNA sequencing, reverse transcription, primer extension, and etc. The dNTP Mix is free of exo-deoxyribonuclease and endo-deoxyribonuclease as well as ribonuclease activity. The dNTP Mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors.
Features
Ideal for PCR amplification and cDNA synthesis
Premixed solution
Nuclease and ribonuclease free
Applications
PCR amplification of DNA fragments
DNA fill-in reaction
DNA sequencing
Reverse transcription
One-step RT-PCR
Storage
-20°C for 36 months
Document
The SMOChem™ Deoxynucleotide (dNTP) Mix is an aqueous solution that contains an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a concentration of 25 mM at pH 8.5. The dNTP Mix is designed for many different molecular biology applications that involved in DNA synthesis or labeling, such as PCR, real-time PCR, DNA sequencing, reverse transcription, primer extension, and etc. The dNTP Mix is free of exo-deoxyribonuclease and endo-deoxyribonuclease as well as ribonuclease activity. The dNTP Mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors.
