Exceptional value for money Rapid detection of all clinically relevant subtypes Positive copy number standard curve for quantification Highly specific detection profile High priming efficiency Broad dynamic detection range (>6 logs) Sensitive to < 100 copies of target
Accurate controls to confirm findings
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Other Products
HCM131 Sorbitol MacConkey Agar Base
Product Info
Document
Product Info
Introduction
Intended Use
For the isolation and differentiation of sorbitol-negative Escherichia coli serotype O157 from food and animal feed and other materials
Principle and Interpretation
Enzymatic digest of casein and enzymatic digest of animal tissue provides nitrogen source, vitamins and growth factors; sodium chloride maintains balanced osmoticpressure; sorbitol is a fermentable sugar; No. 3 bile salt and crystal violet inhibit Gram-positive bacteria, potassiumtellurite inhibits non-o157 Escherichia coli, and cefixime inhibits Proteus; neutral red is a pH indicator; and agar is the coagulant of the culture medium.
Formulation
Ingredients
/liter
Enzymatic digest of casein
17.0g
Enzymatic digest of animal tissue
3.0g
Sorbitol
10.0g
Bile salt No.3
1.5g
Sodium chloride
5.0g
Neutral red
0.03g
Crystal violet
0.001g
Agar
15.0g
pH 7.1±0.2 at 25°C
Preparation
Weigh 51.5g of this product, add 1L of distilled water or deionized water, stir, heat and boil until completely dissolved, dispense into Erlenmeyer flasks, and sterilize at 121℃ for 15min. Melt the sterilized culture medium and cool it to about 45℃, add 1 tube of supporting reagent (SR0240) (0.25mg potassium tellurite and 0.005mg Cefixime) to every 100mL of basal culture medium, mix and pour into the plate.
Quality Control
Cultural characteristics observed after incubation at 35-37°C for 18-24hours
Quality control strains
Growth
Colony color
Escherichia coli ATCC25922
PR≥0.7
pale pink to red
Escherichia coli 0157:H7 NCTC12900
PR≥0.7
colorless
Staphylococcus aureus ATCC 25923
Total inhibition
–
Sorage and Shelf Life
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Document
Intended Use For the isolation and differentiation of sorbitol-negative Escherichia coli serotype O157 from food and animal feed and other materials Principle and Interpretation Enzymatic ……
Functionally Tested in Lateral Flow-made by Attogene in Austin Texas
Specifications 1OD 60nm Gold (1L)
Bulk pricing and manufacturing supply contracts available.
Number of particles/mL
1.5-2 x 1010
Gold Concentration (mg/mL)
3.6-4.1 x 10-2
Molar Concentration (moles/liter)
2.5-3.4 x 10-11
Particle Diameter
60 nm /- 4.5
Document
60nm Colloidal Gold for Lateral Flow is a highly stable and uniform 60 nm gold nanoparticles can be supplied in a range from 1 OD to 100 OD. The quality and performance of a conjugate is critical to successful lateral flow test manufacturing. Our products are made in USA and produced in a state-of-the-art manufacturing facility that enable rapid turnaround times while ensuring batch to batch consistency and reliability.
