Exceptional value for money Rapid detection of all clinically relevant subtypes Positive copy number standard curve for quantification Highly specific detection profile High priming efficiency Broad dynamic detection range (>6 logs) Sensitive to < 100 copies of target
Accurate controls to confirm findings
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Other Products
R4161 HiPure Blood RNA Mini Kit
Product Info
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Product Info
Introduction
The Kit provides fast purification of high-quality RNA from whole blood, cells and tissues using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol / chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. The purified RNA can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection and in vitro translation.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from 1-1.5ml whole blood
Applications
qPCR / RT-PCR, liquid or solid-phasechip analysis, hybridization and SNP detection
The Kit simplifies isolation of RNA from blood with a fast spin-column procedure. Red blood cells are selectively lysed and white cells collected by centrifugation. White cells are then lysed using highly denaturing conditions which immediately inactivate RNases. After homogenization using the DNA spin column, the sample is applied to the RNA column. Total RNA binds to the membrane and contaminants are washed away, leaving pure RNA to be eluted in 30–100µl RNase-free water (provided with the kit) for direct use in any downstream application.
Advantages
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Fast – isolation of several samples can be completed in 20 minutes by using column purification method
Sensitive – RNA can be purified at the level of PG
Kit Contents
Contents
R416102
R416103
Purification Times
50 Preps
250 Preps
HiPure DNA Mini Columns
50
250
HiPure RNA Mini Columns I
50
250
2ml Collection Tubes
100
500
10 x Buffer RBC
50 ml
3 x 100 ml
RTL Lysis Buffer
50 ml
250 ml
Buffer RW1
50 ml
250 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml
Storage and Stability
HiPure Blood RNA Mini Kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form in the RTL Lysis Buffer. Dissolve by warming buffer to 37°C.
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The Kit provides fast purification of high-quality RNA from whole blood, cells and tissues using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol / chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. The purified RNA can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection and in vitro translation.
IST-122 QuickSeal qOpticTM Self Adhesive Sealing Film
Product Info
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Product Info
Overview
Adhesive film with 96 optically clear windows. This seal is peelable, suitable for qPCR and optical applications.
These unique seals combine the strong sealing integrity of our QuickSeal PCR Seal with improved optical properties, thanks to the 96 adhesive-free windows
The seal is made of a durably transparent polyester film, and a strong adhesive is applied across the seal, apart from the 96 round windows
The QuickSeal qOptic seal is recommended for qPCR and other optical applications, such as fluorescence or colorimetric measurements
For all adhesive seals, the best sealing results are achieved using our Hand Roller or KAPS 500 Auto Sealer
Document
Adhesive film with 96 optically clear windows. This seal is peelable, suitable for qPCR and optical applications.
