For the detection of the SARS-CoV-2 variants with the N501Y mutation (UK, South Africa and Brazil)
Rapid detection of specific detection profiles
High priming efficiency
Sensitive to < 100 copies of target
Positive copy number standard curve for quantification
Accurate controls to confirm findings
96 reactions, includes master mix
Our SNPsig® kits use our own proprietary genotyping method to enable the identification of SARS-CoV-2 variants of concern. These products can be used on any real-time PCR machine using familiar protocols, whilst resulting in exceptional genotyping data.
Positive control templates for wild-type and variants are supplied in every kit to make data interpretation simple.
Our SNPsig® technology provides an alternative to sequencing as well as S gene target failure (SGTF) that enables scientists to analyse and monitor these specific genomic mutations. Our kits can provide a pivotal role in screening for SARS-CoV-2 variants for the purpose of genomic surveillance and studies.
Other Products
[RP1000] ExcelRT™ Reverse Transcriptase, 200 U/μl, 20000 U
Product Info
Document
Product Info
Description
The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase – an RNA dependent DNA polymerase capable of generating first strand cDNA using an RNA template. It is designed to reduce RNase H activity and create better thermal stability. The ExcelRT™ Reverse Transcriptase is able to routinely synthesize first strand cDNA >8 kb at 37~50°C.
Features
High yield
Thermostable, up to 50°C, during first strand synthesis
High processivity, generating cDNA up to 8 kb
Reduced RNase H ribonuclease activity
Application
Generation of first strand cDNA from total RNA or mRNA.
Suitable for generating cDNA from RNA with strong secondary structure which can be reduced at temperature up to 50°C.
Storage
-20°C for 24 months
High yield
Thermostable, up to 50°C, during first strand synthesis
20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol
5X RT buffer
250 mM Tris-HCl (pH 8.3 at 25°C), 375 mM KCl and 15 mM MgCl2
Storage
-20°C for 24 months
Document
The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase – an RNA dependent DNA polymerase capable of generating first strand cDNA using an RNA template. It is designed to reduce RNase H activity and create better thermal stability. The ExcelRT™ Reverse Transcriptase is able to routinely synthesize first strand cDNA >8 kb at 37~50°C.
Eight discrete bands, ranging from 100 to 1,000 bases
Higher intensity band at 500 bases for easy reference
No need for staining or destaining as loading dye contains ethidium bromide
Convenient lyophilized format provides better product stability
The Norgen 100 b RNA Ladder is a set of RNA transcripts derived from recombinant DNA templates. This ladder is suitable for precise sizing of small RNA molecules using native or denaturing agarose gels, and can be easily visualized under UV.
To reconstitute the lyophilized RNA ladder, add 250 µL of 1x loading buffer to each 25 loads vial and vortex gently. Heat at 80°C for 10 minutes. Incubate on ice for 1 min. Load 10 µL on a 1.5-2% gel. For optimal results, use Norgen 2x loading buffer with each RNA sample. There is no need for staining and destaining denaturing gels since Norgen’s loading buffer contains ethidium bromide.
Propargyl-PEG2-alcohol has propargyl and alcohol end groups. The propargyl can participate in copper-catalyzed Click Chemistry reactions with azides. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG2-alcohol has propargyl and alcohol end groups. The propargyl can participate in copper-catalyzed Click Chemistry reactions with azides. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
