The EzRNA™ RNA Capping System is a user-friendly product for post-transcriptional RNA modification. Both Vaccinia Capping Enzyme and 2’-O-Methyltransferase are included in the package, which are able to perform in a single reaction. The Vaccinia Capping Enzyme attach 7-methylguanylate cap (m7Gppp, Cap-0) to the 5′ end of RNA to form m7Gppp5’N-RNA (Cap-0 RNA). The 2′-O-methyltransferase utilizes Cap-0 RNA as a substrate, employing S-adenosine methionine (SAM) as a methyl donor to methylate 2′ -OH of the first nucleotide at the 5′ end of Cap-0 RNA, resulting in the formation of Cap-1 RNA.
Detail
Description
The EzRNA™ RNA Capping System is a user-friendly product for post-transcriptional RNA modification. Both Vaccinia Capping Enzyme and 2’-O-Methyltransferase are included in the package, which are able to perform in a single reaction. The Vaccinia Capping Enzyme attach 7-methylguanylate cap (m7Gppp, Cap-0) to the 5′ end of RNA to form m7Gppp5’N-RNA (Cap-0 RNA). The 2′-O-methyltransferase utilizes Cap-0 RNA as a substrate, employing S-adenosine methionine (SAM) as a methyl donor to methylate 2′ -OH of the first nucleotide at the 5′ end of Cap-0 RNA, resulting in the formation of Cap-1 RNA.
Features
2’-O-Methyltransferase included for Cap-1 RNA
High capping efficiency
High stability
RNase inhibitor is included to enhance the stability of capping reaction
Application
Generation of 5’Cap-0 (m7Gppp) and Cap-1 (m7GpppNm-) RNA by enzymatic reaction
mRNA synthesis for in vitro translation
Gene expression studies
mRNA vaccine development and therapeutics
Storage
-20°C for 24 months
Other Products
D3180 HiPure Circulating Nucleic Acid Micro Kit
Product Info
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Product Info
Introduction
Free-circulating nucleic acids, such as tumor-specific extracellular nucleic acid fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp(Nucleic Acid). The HiPure Circulating Nucleic acid Micro Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. Samples can be either fresh or frozen (provided that they have not been frozen and thawed more than once). Free-circulating cell-free DNA, RNA or viral nucleic acids are eluted in Nuclease Free Water, ready for use in amplification reactions or storage at -30 to -15°C. Purified nucleic acids are free of proteins, nucleases, and other impurities.
Details
Specifications
Features
Specifications
Main Functions
Isolation circulating DNA from 0.6ml plasma,serum, body fluids
Applications
qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Serum, plasma and other cell-free fluid samples
Sample amount
0.6ml
Elution volume
≥30μl
Time per run
≤40 minutes
Liquid carrying volume per column
800μl
Binding yield of column
100μg
Principle
This product is based onsilica column purification. The sample is lysed and digested with lysate andprotease, DNA is released into the lysate. Transfer to an adsorption plate andfilter column. Nucleic acid is adsorbed on the membrane, while protein is notadsorbed and is removed with filtration. After washing proteins and otherimpurities, Nucleic acid was finally eluted with low-salt buffer.
Advantages
High yield – most optimal process, free DNA (>50bp) can be obtained to the maximum extent
High concentration – low elution volume, ensuring high nucleic acid concentration
High purity – low alcohol binding method, completely removing inhibitor and protein pollution
High recovery – DNA can be recoveredat the level of PG by silica gel column purification
Kit Contents
Contents
D318002
D318003
Purification Times
50 Preps
250 Preps
Buffer ACL
40 ml
200 ml
Buffer DCW1
22 ml
110 ml
Buffer DCW2*
20 ml
2 x 50 ml
Proteinase K
34 mg
180 mg
Protease Dissolve Buffer
1.8 ml
10 ml
Carrier RNA
110 μg
310 μg
Nuclease Free Water
10 ml
30 ml
HiPure CFDNA Mini Columns
50
250
2 ml Collection Tubes
100
5 x 100
Storage and Stability
Proteinase K, Carrier RNAshould be stored at 2-8°C upon arrival. However, short-term storage (up to 12weeks) at room temperature (15-25°C) does not affect their performance. Theremaining kit components can be stored dry at room temperature (15-25°C) andare stable for at least 18 months under these conditions. The entire kit can bestored at 2-8°C, but in this case buffers should be redissolved before use.Make sure that all buffers are at room temperature when used.
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Free-circulating nucleic acids, such as tumor-specific extracellular nucleic acid fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments,
The Opentrons HEPA Module enables you to run sensitive contamination-prone applications. It removes 99.99% of 0.3 μm DNA-containing particulates and biological contaminants like bacteria, fungi, and other microorganisms from the air, creating a clean work environment inside the OT-2.
The Opentrons HEPA Module comes in two different voltages. Your account manager will help you choose the correct one for your geographic region.
Document
The Opentrons HEPA Module enables you to run sensitive contamination-prone applications. It removes 99.99% of 0.3 μm DNA-containing particulates and biological contaminants like bacteria, fungi, and other microorganisms from the air, creating a clean work environment inside the OT-2.
The Opentrons HEPA Module comes in two different voltages. Your account manager will help you choose the correct one for your geographic region.
N-Me-N-bis(PEG2-propargyl) has two alkyne groups which can participate in Click Chemistry reactions with azide-bearing compounds or biomolecules; copper is required for catalyzation. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
N-Me-N-bis(PEG2-propargyl) has two alkyne groups which can participate in Click Chemistry reactions with azide-bearing compounds or biomolecules; copper is required for catalyzation. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
