For selective isolation and culture of Staphylococcus aureus.
Principle:
Peptone and beef extract powder provides carbon, nitrogen, vitamins and minerals; D- mannitol to fermentable sugars; higher levels of sodium chloride to provide a higher osmotic pressure, suppress most non-staphylococcal microorganisms ; phenolsulfonphthalein as pH indicator; agar is medium coagulant. Typical pathogenic staphylococci (coagulase positive) D- mannitol produce acid fermentation and produce yellow colonies with a yellow halo, typically non-pathogenic Staphylococcus unfermented D- mannitol to form red colonies.
Formulation(per liter): Pancreatic digest of casein 5.0g Pancreatic digest of animal tissue 5.0g Beef Extract 1.0g Sodium Chloride 75.0g Mannitol 10.0g Phenol Red 0.025g Agar 15.0g Final PH 7.4±0.2
How to use： 1.Suspend 111g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121℃ for 15 minutes. 2.Diluted and treated samples.
Quality control：
Item
The name and number of strain
Growth
Colony Color
1
Staphylococcus aureus CMCC (B) 26003
Good
Golden yellow
2
Staphylococcus epidermidis CMCC (B) 26069
Good
Red
3
Escherichia coli CMCC (B) 44102
Inhibition
—
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 250g/bottle
Other Products
Urine Collection and Preservation Devices
Product Info
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Product Info
Overview
RNA/microRNA/DNA/Proteins are preserved for more than 2 years at room temperature in Norgen’s Urine Preservative
Compatible with most DNA, Total RNA, microRNA and protein isolation methods
Preservative is available in a single dose liquid format (ampule)
Preservative is also available in a dried format in tubes – Urine Collection and Preservation Tubes
Urine Collection and Preservation Devices are available in 4 convenient sizes: 5 cc tubes, 15 cc tubes, 50 cc tubes, and 120 cc cups
Norgen’s Urine Preservative is designed for the rapid preservation of DNA, RNA, microRNA and proteins from fresh urine samples. In addition, the Urine Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins.
Norgen’s Urine Preservative is available in 2 convenient formats:
1. Urine Preservative Single Dose Ampules With this product Norgen’s Urine Preservative is supplied in a liquid format in single dose ampules. The user simply collects 5 – 50 mL of urine into a urine collection container and adds the contents of the Urine Preservative Single Dose (Cat# 18126). The urine and preservative are then mixed, and the urine nucleic acids and proteins are preserved at room temperature.
2. Urine Collection and Preservation Tubes Norgen’s Urine Preservative is also available in a dried format in Norgen’s Urine Collection and Preservation Tubes. The user simply collects urine into the tubes and mixes gently until the orange preservative pellet in the tube has dissolved. Norgen’s Urine Collection and Preservation Tubes are available in 3 convenient sizes:
Select Urine Tested with the Norgen Urine Collection and Preservation Tubes
Human Mouse Lynx Wolf Urine collected from snow
Available Sizes and Formats
Urine Collection and Preservation Tubes (15 cc) – Urine inputs from 5 – 15 mL
Storage Conditions and Product Stability All tubes should be kept tightly sealed and stored at room temperature (15 – 25°C) for up to 2 years without any reduction in performance.
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from insect tissue
Applications
PCR, southern bolt and virus detection, etc
Purification method
96 well DNA plate
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Insect tissue samples
Sample amount
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column
Principles
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High purity DNA – can be used in sensitive downstream applications such as multiplex and quantitative pcr
Good repeatability – suitable for extracting high-yield DNA from insect tissue samples
Safe – without phenol chloroform extraction and alcohol precipitation
Kit Contents
Contents
D313901
D313902
Purification Times
1 x 96
4 x 96
HiPure DNA Plate
1
4
2.2 ml Collection Plate
1
4
1.6 ml Collection Plate
1
4
0.5ml Collection Plate
1
4
Seal Film
8
32
Buffer ITL
30 ml
120 ml
Buffer IL
30 ml
125 ml
Buffer GW1
44 ml
2 x 110 ml
Buffer GW2
50 ml
3 x 50 ml
Proteinase K
50 mg
200 mg
Protease Dissolve Buffer
6 ml
15 ml
Buffer AE
20 ml
60 ml
Storage and Stability
Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.
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HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
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Exceptional value for money Rapid detection of all clinically relevant subtypes Positive copy number standard curve for quantification Highly specific detection profile High priming efficiency Broad dynamic detection range (>6 logs) Sensitive to < 100 copies of target
Accurate controls to confirm findings
50 & 150 reactions
