TDL5E Bench top low speed refrigerated centrifuge machine

Application:

Widely used in the field of biochemistry, biological products, pharmaceutical factory and laboratory.

TDL5E Technical Parameters:

Max. Speed	5000rpm	Max.RCF	4420xg
Max. Volume	4x180ml	Speed Accuracy	±20rpm
Timer	1~99h59min	Noise	≤60dBA
Dimension(LxWxHmm)	570x600x340mm	Net Weight	76Kg
Power Supply	AC220V/110V 50HZ/60HZ	Temperature Range	-20℃~40℃
Temperature Accuracy	±1℃	Certifications	CE, ISO & Calibration report is available.

Matched Rotors for TDL5E:

Order No.	Rotor Type	Max. Speed(rpm)	Volume(ml)	Max.RCF(xg)
5E-1	Swing rotor	4000	4x180ml	2580
5E-2	Microplate rotor	4000	2x3x96well	2020
5E-3	Swing rotor	5000	4x1x50ml	4420
5E-4	Swing rotor	5000	4x1x100ml	4420
5E-5	Swing rotor	4000	4x2x50ml	2820
5E-6	Swing rotor	4000	4x4x15ml	2820
5E-7	Swing rotor	4000	4x6x10/7ml	2580
5E-8	Swing rotor	4000	4x6x5ml	2170
5E-9	Swing rotor	4000	4x4x10/7ml	2580
5E-10	Swing rotor	4000	4x4x5ml	2170
5E-11	Fixed rotor	5000	6x15ml	2540
5E-12	Fixed rotor	5000	12x15ml	3080
5E-13	Fixed rotor	5000	24x15ml	3500
5E-14	Fixed rotor	5000	4x50ml	2520
5E-15	Fixed rotor	5000	6x50ml	2850
5E-16	Fixed rotor	5000	4x100ml	2630
5E-17	Fixed rotor	5000	6x100ml	3130

TDL5E is a table top low speed refrigerated centrifuge widely used in the field of biochemistry, biological products, pharmaceutical factory and laboratory.

Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 1-10μl blood, <10mg tissue, urine, blood stain, seminal stain
Applications	PCR, southern bolt and virus detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Animal tissues, blood stain, urine, seminal stain and various forensic samples
Sample amount	Blood:1-100μl, Tissue:<10mg
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).

Advantages

• Fast – several samples can be extracted in 20 minutes (after digestion)
• High purity – purified DNA can be directly used in various downstream applications
• High recovery – DNA can be recovered at the level of PG
• Good repeatability – silica technology can obtain ideal results every time

Kit Contents

Contents	D312502	D312503
Purification Times	50 Preps	250 Preps
Buffer ATL	15 ml	60 ml
Buffer AL	15 ml	60 ml
Buffer GW1*	22 ml	66 ml
Buffer GW2*	20 ml	2 x 50 ml
Carrier RNA	310 μg	2 x 310 µg
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	1.8 ml	10 ml
Buffer AE	15 ml	60 ml
HiPure DNA Mini Columns I	50	2 x 125
2 ml Collection Tubes	100	5 x 100

Storage and Stability

Carrier RNA and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.

Experiment Data

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.