1 kb DNA Ladder in Ready-to-load format.

 K-PECID

SKU: 700004325

500 assays per kit

Content:	500 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: Ambient, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Pectin
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	235
Signal Response:	Increase
Reaction Time (min):	~ 30 min
Application examples:	Food ingredients (e.g. citrus fruit and apple) and other materials.

The Pectin Identification Assay Kit is suitable for the identification of pectin in food ingredients. This kit now employs a new pectate lyase from Aspergillus niger.

See the complete list of our polysaccharide assay kit products.

Advantages

• Very cost effective 
• All reagents stable for > 2 years after preparation 
• Only enzymatic kit available 
• Simple format 
• Standard included

The Pectin Identification Assay Kit is suitable for the identification of pectin in food ingredients. This kit now employs a new pectate lyase from Aspergillus niger.

Overview

• Purification and enrichment of intact plasma/serum exosomes for functional studies
• Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
• Versatile plasma/serum input volume
• No phenol extractions, Proteinase K treatment, nor carrier RNA required
• No time-consuming ultracentrifugation, filtration nor special syringes required
• No precipitation reagents, nor overnight incubation required
• Compatible with plasma/serum from most species
• Pure exosomes are purified and are free from any other RNA-binding proteins
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin
• The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services

Norgen’s Plasma/Serum Exosome and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the sequential isolation of exosomal RNA from different plasma/serum sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Norgen’s Plasma/Serum Exosome and RNA Isolation Mini Kit

For sample volumes ranging from 50 µL to 1 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Norgen’s Plasma/Serum Exosome and RNA Isolation Midi Kit

For sample volumes ranging from 1 mL to 4 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Norgen’s Plasma/Serum Exosome and RNA Isolation Maxi Kit

For sample volumes ranging from 4 mL to 10 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Details

Supporting Data

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Kit Specifications
Minimum Plasma/Serum Input	50 μL
Maximum Plasma/Serum Input	1 mL
Size of Exosomes Purified	40 nm – 150 nm
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35 – 40 minutes
Average Yields*	Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

Component	Cat. 58300 (50 preps)	Cat. 58500 (25 preps)	Cat. 58600 (15 preps)
Slurry E	12.5 mL	12.5 mL	12.5 mL
ExoC Buffer	8 mL	8 mL	2 x 8 mL
ExoR Buffer	12 mL	12 mL	12 mL
Lysis Buffer A	20 mL	20 mL	20 mL
Lysis Additive B	2 mL	2 mL	2 mL
Wash Solution A	38 mL	18 mL	18 mL
Elution Solution A	6 mL	6 mL	6 mL
Mini Filter Spin Columns	50	25	15
Mini Spin Columns	50	25	15
Collection Tubes	50	25	15
Elution Tubes (1.7 mL)	100	50	30
Product Insert	1	1	1

Features 

1. TD4A centrifuge is of microprocessor control, less noisily, it is widely used to qualitative analysis of blood serum, plasma and urea in the fields of hospital, blood center, laboratory and biochemistry.

2. Brushless motor, free maintenance, no powder pollution, quick in speed up and down.

3. The flexible axle driven system which drive the rotor directly, smooth in operation and small vibration.

4. There are many rotors for your choice, suitable for different specifications meet customers’ different requirements of separation.

5. Micro computer control system, LED display the RCF，time and speed. 

6 .Electric lid lock, compact design, super speed and imbalance protection.

 TD4A Technical Parameter：

Max. Speed	4000rpm
Max. RCF	2250×g
Max. Capacity	6×50ml
Time Range	0~99min
RPM/RCF Convert	Yes
Noise (dB)	≤ 45
Temperature	Normal
Acc/Dec	10 Kinds
Speed Accuracy	±10r/min
Voltage(V/Hz)	AC 220V/110V  50HZ/60HZ
Size (W x D x Hmm)	485×320×255mm
Net Weight/Gross Weight(Kg)	18KG/21KG
Certificates	CE,ISO & Calibration report are available

 Matched Rotors for TD4A 

Order No	Rotors	Max speed r/min	Volume(ml)	Max RCF(g)
No31601	Angle Rotor	4000	12×10ml Vacuum tube	2150
			12×7ml Vacuum tube adapter
			12×5ml Vacuum tube adapter
No31605	Angle rotor	4000	30×7/5ml	2250
No31604	Angle rotor	4000	18×10ml	2250
No31607	Angle rotor	4000	24x10ml	2200
No31602	Angle rotor	4000	12×15ml/7ml/5ml	2150
No31603	Angle rotor	4000	12×20ml	2220
No31606	Angle rotor	4000	6x50ml	2100