This product is specially designed for stool RNA extraction. The kit is suitable for extracting high-purity microbial or host cell RNA from ≤ 0.15g stool samples. The purified RNA can be directly used in RT-PCR and Northern hybridization.
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.
Kit Contents
| Contents | R662601 | R662602 | R662603 |
| Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
| MagPure RNA Particles | 1.7 ml | 4.0 ml | 18 ml |
| DNase I | 600 μl | 2 x 600 μl | 10 x 600 μl |
| DNase Buffer | 30 ml | 40 ml | 200 ml |
| Buffer SPL | 30 ml | 60 ml | 270 ml |
| Buffer PHC | 30 ml | 60 ml | 270 ml |
| Buffer MCB* | 9 ml | 15 ml | 75 ml |
| Buffer ALB3* | 10 ml | 20 ml | 100 ml |
| Buffer GW1* | 44 ml | 66 ml | 2 x 220 ml |
| Buffer RW2* | 20 ml | 50 ml | 2 x 100 ml |
| RNase Free Water | 10 ml | 30 ml | 120 ml |
| 2ml Bead Tubes | 48 | 96 | 5 x 96 |
Storage and Stability
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
This product is specially designed for stool RNA extraction. The kit is suitable for extracting high-purity microbial or host cell RNA from ≤ 0.15g stool samples. The purified RNA can be directly used in RT-PCR and Northern hybridization.
bis-PEG2-endo-BCN is a click chemistry reagent, The BCN group enable copper free click chemitry with azide-tagged molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
bis-PEG2-endo-BCN is a click chemistry reagent, The BCN group enable copper free click chemitry with azide-tagged molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Attogene’s Lead Rapid Lab Lateral Flow Kit can be used for the screening of Lead in water and food samples at greater than or equal to 10 ppb in a laboratory setting. Unlike the field-based kit, the lab kit is intended for a more technical end user who will be evaluating samples in a laboratory setting.
Lead contamination is a serious worldwide environmental problem. As it is difficult to detoxify by chemical or biological methods, gradual Lead ion accumulation in the nervous and cardiovascular systems of the human body can subsequently cause serious diseases. Long-term health consequences of drinking Lead-contaminated food and water include brain, heart, kidney, lungs and immune system problems for adults, and the physical and mental development delays in infants and children. Attogene’s Lead Lateral Flow test gives results conforming of 10ppb or greater in 5-15 minutes.
This is a revolutionary product designed to make Lead testing in easy and affordable. This fast screening test kit contains 10 tests with everything needed for accurate results of unsafe lead levels. With reliable results in only 10 minutes, this test kit clearly gives results confirming Lead in water and conforming to the EPA guideline of 15 ppb (µg/L).
Sample Collection:
1. Take a first-draw sample
Immediately after opening a faucet or valve, collect a 250 mL sample. This sample should be from each tap used for consumption.
2. Take a flush sample
If first-draw sample results show elevated lead levels of 5 ppb or higher, collect a flush sample. To do this, ensure water has not been used for between 8 to 18 hours, then collect the sample at 30 seconds.
3. Take sequential samples
If you want to test a lead service line, collect 8 to 10 sequential samples, depending on how far the line is from the tap.
Standards and Regulations and recommendations for Lead
High Blood lead levels (i.e., greater than 700ppb) can cause serious health effects, including seizure, coma, and death. Blood levels as low as 100ppb have been associated with adverse effects on cognitive development, growth, and behavior among children aged 1-5 years.
Screening of Lead in water samples at 5-10 ppb
Format: 10 tests (5 tests/5 controls)
Run Time: 15 Minutes