DBCO STP ester is a water-soluble reagent with a terminal DBCO group and a STP ester group. DBCO STP ester that can be used for the modification of peptides, antibodies, proteins, and other molecules containing the amine group. The STP esters are excellent alternatives to conventional N-hydroxysuccinimide (NHS) esters for coupling reactions in aqueous environments. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. Reagent grade, for research use only.
DBCO STP ester is a water-soluble reagent with a terminal DBCO group and a STP ester group. DBCO STP ester that can be used for the modification of peptides, antibodies, proteins, and other molecules containing the amine group. The STP esters are excellent alternatives to conventional N-hydroxysuccinimide (NHS) esters for coupling reactions in aqueous environments. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. Reagent grade, for research use only.
Attogene has developed the first of its kind and patent pending technology that uses a novel RNA substrate tagged on the 5′ and 3′ end with a Biotin that is attached to our streptavidin colloidal gold reporter molecules and a test line tag. In the absence of RNases, the gold tagged RNA molecule will flow up the lateral flow strip and bind to the test line using an anti tag antibody (INDICATING that NO RNase is detected). When RNases are present, however, the RNA substrate is cleaved, and the 5′ and 3′ tags are spatially separated in solution cauing the test line to disapear.
This test can be used to rapidly and efficiently detect ribonucleases in both liquid and on solid surfaces and a perfect tool for monitoring mRNA vaccine manufacturing.
RNase activity in a convenient and sensitive colormetric assay that delivers results in real time. Great for Quality Testing for RNase contamination of materials and supplies.
Norgen’s RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen’s RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen’s RNA purification kits. Alternatively, after isolating total RNA using one of Norgen’s RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen’s RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.
Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme and enzyme incubation buffer for 50 or 200 reactions.
Storage Conditions
The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8ºC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR and Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20ºC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.
| Component | Cat. 25710 (50 rxns) | Cat. 25720 (200 rxns) |
|---|---|---|
| DNase I | 1 vial / 1,600 units | 4 vials (1,600 units/vial) |
| Buffer DR | 1 mL | 4 x 1 mL |
| Enzyme Incubation Buffer | 6 mL | 4 x 6 mL |
| Product Insert | 1 | 1 |