TruScript Reverse Transcriptase is Available in Three Convenient Formats:
1. TruScript Reverse Transcriptase Kit (Cat.#54440)
This kit contains 5X RT Buffer and a vial of TruScript Enzyme Mix (200 units/µL). This enzyme can be used for reverse-transcription reactions with any user-supplied primers.
2. TruScript First Strand cDNA Synthesis Kit (Cat.#54420)
This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts).
The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and primers (oligo dT and random hexamers) for effective cDNA synthesis from total RNA transcripts.
3. TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.#54400)
This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of messenger RNA (poly A-containing transcripts).
The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and oligo dT primer for the effective cDNA synthesis from total RNA transcripts or enriched mRNA sample.
TruScript Reverse Transcriptase | TruScript First Strand cDNA Synthesis Kit for mRNA | TruScript First Strand cDNA Synthesis Kit | |
Kit contains only reverse transcriptase enzyme and buffer (no primers or other buffer components) | |||
Your template is enriched mRNA | |||
Your template is total RNA (poly A OR non-poly A-containing transcripts) | |||
Kit contains oligo dT | |||
Kit contains both oligo-dT primer and Random Hexamers | |||
Kit contains reaction buffer with nucleotides and primer | with oligo-dT | with oligo-dT and random hexamers | |
You have your own first strand synthesis primer | |||
Your template is microRNA (using your own primers) | |||
Cat.#54440 | Cat.#54400 | Cat.#54420 |
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TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.# 54400) – Kit Components
1. TruScript Enzyme Mix
2. 2x Reaction Mix
3. Nuclease-Free Water
4. Product Insert
Storage Conditions and Product Stability
Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.
Component | Cat. 54440 (10,000 units) | Cat. 54420 (50 rxns) | Cat. 54410 (50 rxns) |
---|---|---|---|
TruScript™ Reverse Transcriptase (200 units/ μL) | 50 μL | – | – |
5x RT Buffer | 1 mL | – | – |
TruScript™ Enzyme Mix | – | 100 μL | 100 μL |
2x Reaction Mix | – | 500 μL | – |
2x Reaction Mix for mRNA | – | – | 500 μL |
Nuclease-Free Water | 1.25 mL | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 | 1 |
Grey, standard profile semi-skirted PCR plate with a rigid two-component hardshell design which eliminates warping and distortion during PCR testing.
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