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Name of Product

Trichinella spiralis – IgG ELISA

Catalog Number

9750

Short Info

Trichinellosis is caused by Trichinella spiralis, a parasitic nematode of pork, horse and wild animals. Humans can be infected by eating raw or undercooked meat from infected animals. Most infected people do not show any symptoms. However, in some cases, symptoms appear during intestinal stage (diarrhea and abdominal pain) and muscular stage (muscle pain, fever, edema). Diagnosis is based on signs and symptoms, but in many cases it is not conclusive for the diagnosis. Serological assays have an important place on the final diagnosis of the disease. The ELISA kit has shown appropriate performance for the serological screening of trichinellosis.

This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.

Method/Platform

ELISA in microplate format

Range/Assay Sensivity

95% sensitivity, 98% specificity

Test Principle

The kit provides all the material needed to perform 96 enzyme-linked immunosorbent assays (ELISA) on breakable microtitration wells sensitized with Trichinella spiralis excreted/secreted (E/S) larval antigens. Specific antibodies in the sample will bind to these antigens and washing will remove unspecific antibodies. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate. A second washing step will remove unbound conjugate. Revealing bound antibodies is made by the addition of pNPP substrate which turns yellow in the presence of alkaline phosphatase. Color intensity is proportional to the amount of Trichinella spiralis specific antibodies in the sample. Potassium phosphate is added to stop the reaction. Absorbance at 405 nm is read using an ELISA microplate reader. The test can be performed with automatic systems, but this must be validated by the user.

Brief Instructions

Storage

2-8° C

Components

ELISA wells, dilution buffer, washing solution, control sera, conjugate, substrate solution, stopping solution

Trichinellosis is caused by Trichinella spiralis, a parasitic nematode of pork, horse and wild animals. Humans can be infected by eating raw or undercooked meat from infected animals. Most infected people do not show any symptoms. However, in some cases, symptoms appear during intestinal stage (diarrhea and abdominal pain) and muscular stage (muscle pain, fever, edema). Diagnosis is based on signs and symptoms, but in many cases it is not conclusive for the diagnosis. Serological assays have an important place on the final diagnosis of the disease. The ELISA kit has shown appropriate performance for the serological screening of trichinellosis.

NEST 2.0 mL Microcentrifuge Tube, polypropylene, sterile, DNase, RNase, pyrogen and endotoxin free. Relative centrifugal force (RCF): 30000 x g. Easy-open, secure safelock cap seal. 500 count.

Labware definition is available for immediate use in Opentron’s Labware Library.

NEST 2.0 mL Microcentrifuge Tube, polypropylene, sterile, DNase, RNase, pyrogen and endotoxin free. Relative centrifugal force (RCF): 30000 x g. Easy-open, secure safelock cap seal. 500 count.

Labware definition is available for immediate use in Opentron’s Labware Library.

Overview

• Milk samples are stable for 1 month at room temperature (or 1 week at 37°C) in the Preservation Solution
• Fast and easy processing using a rapid spin-column format
• DNA can be isolated and detected from as little as 100 µL of milk
• Isolate high quality genomic DNA

Norgen’s Milk DNA Preservation and Isolation Kit is an all-in-one solution designed for 1) preservation of DNA in milk samples at ambient temperature; and 2) isolation of high quality DNA within a laboratory setting. The kit is provided with a Preservation Solution that allows for either immediate processing of milk samples, or allows for the storage of the milk samples for up to 1 month at room temperature prior to DNA isolation. Genomic DNA extracted from somatic cells and bacteria found in milk can be used in various applications. The isolated DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Milk DNA can also be used to diagnose particular types of infections. Milk DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream applications including PCR, Southern Blot analysis, sequencing and microarray analysis.

Milk DNA Preservative

Norgen’s Milk DNA Preservation Solution is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of DNA from fresh specimens. The preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the preservative allow samples to be stored for 1 month without any detectable DNA degradation.

Details

Supporting Data

Figure 1 / 2

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Kit Specifications
Maximum Milk Input	0.5 mL
Time to Complete 10 Purifications	80 minutes (20 minutes hands on)

Storage Conditions and Product Stability
The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Storage in Preservation Buffer
1) Storage at -20°C is recommended for archival samples and will provide optimal preservation. The preservation buffer will freeze at -20°C. Samples can be stored indefinitely at -70°C.
2) Samples can be stored at room temperature (25°C) for up to 1 month without significant loss of DNA quality.
3) DNA has also been successfully isolated from samples stored at 37°C 1 for one week.

Kit Components	Cat. 44800 (25 preps)
Preservation Solution E	30 mL
Proteinase K	0.6 mL
Purification Additive	0.5 mL
Buffer SK	40 mL
Wash Solution A	18 mL
Elution Buffer B	8 mL
Mini Spin Columns	25
Collection Tubes	25
Elution Tubes (1.7 mL)	25
Product Insert	1