Product Description

Product Detail

Kit Storage and Term of Validity

 Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

 Term of Validity: 14 months

Isothermal Nucleic Acid Principle Summary

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room   temperature and constant temperature (generally 39℃~42℃), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex;perform a homology search and bind the target homology domain,at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation,the polymerase also binds to the 3′ end of the primer, initiating chain extension. At the same time, relying on the function of exonuclease, adding specific molecular probes designed according to the template, and using fluorescence monitoring equipment can realize real-time monitoring of the amplification process of the target fragment.

Isothermal Nucleic Acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Parameters	Details
Product Name	RNA Isothermal Amplification Kit EXO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal Nucleic Acid Applications

Suitable for RNA isothermal rapid amplification kit(Fluorescent type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

RNA fluorescent kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

Introduction

The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The Purified DNA/RNA is used for RT-PCR and PCR detection.

Details

Specifications

Features	Specifications
Main FunctionsC	Co-isolation DNA and RNA from a single FFPE tissue sample
Applications	RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	FFPE slice, FFPE embedded tissue
Sample amount	No more than six 10µm sections of 150mm2 surface area or three 20µm sections of 150mm2 surface area.

Principle

FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. After centrifugation, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. For RNA purification, transfer RNA Lysate to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-salt buffer. For DNA purification, transfer DNA Lysate to an adsorption column and DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA was finally eluted with low-salt buffer.

Advantages

1.Use non-toxic dewaxing solution without contact with xylene
2.Obtain both DNA and RNA simultaneously from the same sample. Elute separately without affecting each other (Have the same steps and effects as top brand 80234, perfect substitute.)

Kit Contents

Contents	IVD5116
Purification Times	50 Preps
HiPure DNA Micro Column	50
HiPure RNA Mini Column I	50
2ml Collection Tubes	150
Proteinase K	50 mg
Protease Dissolve Buffer	5 ml
Buffer DPS	60 ml
Buffer FRL	15 ml
Buffer ATL	15 ml
Buffer RLC	15 ml
Buffer AL	15 ml
Buffer VHB	44 ml
Buffer RW2	25 ml
RNase Free Water	10 ml
Buffer AE	10 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data

The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The Purified DNA/RNA is used for RT-PCR and PCR detection.

Introduction

HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing,restriction digestion, or various labeling reactions. In addition, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.

Details

Specifications

Features	Specifications
Main Functions	Recover DNA fragments between 20bp-20kbp from crude DNA and enzymatic reaction solution
Applications	PCR, sequencing, labeling reactions, ligations and restriction digestion, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Crude DNA, enzymatic reaction solution
Sample amount	<150μl
Recovery	80%
Elution volume	≥15μl
Time per run	≤10 minutes
Liquid carrying volume per column	800µl
Binding yield of column	20µg

Principle

The HiPure system uses a simple bind-wash-elute procedure. Gelslices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Advantages

• High recovery efficiency – up to 80% DNA recovery
• Fast – isolation can be completed in 10 minutes by column gel method
• Low elution volume – elution volume can be as low as 10μl
• Efficient – efficient removal of various inhibitory factors

Kit Contents

Contents	D214102	D214103
Purification Times	100 Preps	250 Preps
Buffer GWP	30 ml	60 ml
Buffer DW2	20 ml	50 ml
Elution Buffer	30 ml	60 ml
HiPure DNA Mini Columns I	50	250
2 ml Collection Tubes	50	250

Storage and Stability

HiPure DNA Clean Up Kit components are guaranteed for at least one year when stored at room temperature. If any precipitates form in the buffers, warm at 37℃ to dissolve.

HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing,restriction digestion, or various labeling reactions. In addition, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.