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Disulfide Biotin alkyne is an azide-activated cleavable biotin probe that allows for efficient recovery of avidin-bound protein complexes in affinity-based assays. This reagent contains a biotin moiety linked to an alkyne group through a spacer arm containing a cleavable disulfide linker. Under reducing conditions (50 mM dithiothreitol, 10 mM 2-mercaptoethanol or 1% sodium borohydride), the disulfide bonds are cleaved, releasing the biotin tag and any avidin conjugate bound to it.

Disulfide Biotin alkyne is an azide-activated cleavable biotin probe that allows for efficient recovery of avidin-bound protein complexes in affinity-based assays. This reagent contains a biotin moiety linked to an alkyne group through a spacer arm containing a cleavable disulfide linker. Under reducing conditions (50 mM dithiothreitol, 10 mM 2-mercaptoethanol or 1% sodium borohydride), the disulfide bonds are cleaved, releasing the biotin tag and any avidin conjugate bound to it.

Introduction

Intended Use

For enriching bile-tolerant Gram-negative bacteria in the microbiological examination of pharmaceutical products.

Principle and Interpretation

Pancreatic digest of gelatin provide protein, vitamins and amino acids; glucose carbon source; disodium hydrogen phosphate and potassium dihydrogen phosphate as a buffer; ox bile and brilliant green as selective antibacterial agent, inhibiting the growth of non-Enterobacteriaceae.

Formulation

Ingredients	/liter
Pancreatic digest of gelatin	10g
Glucose monohydrate	5g
Dehydrated ox bile	20g
Potassium dihydrogen phosphate	2g
Disodium hydrogen phosphate dihydrate	8g
Brilliant green	15mg
pH7.2±0.2 at 25°C

Preparation

Suspend 45g in 1L of distilled water,stir until completely dissolved and dispense 100 mL into test tubes , heat at 100 °C for 30 min in a waterbath or flowing steam.

Quality Control

The following quality control strains were inoculated and cultured at 30-35℃ for 24h-48h. The results are as follows:

Quality control strains	Inoculum (CFU)	Growth
Escherichia coli ATCC8739	< 100	Good growth
Pseudomonas aeruginosa ATCC9027
Staphylococcus aureus ATCC6538	> 100	Inhibited

Storage and Shelf Life

2-30℃，Keep container tightly closed, avoid direct sunlight.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for

30 minutes.

Intended Use For enriching bile-tolerant Gram-negative bacteria in the microbiological examination of pharmaceutical products. Principle and Interpretation Pancreatic digest of gelatin pro……