96S Super Magnet Plate

Overview

• Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more
• Simple and quick workflow: library could be prepared in less than 5 hours
• Component of Norgen’s metagenomics workflow
• A single NGS run can be prepared with up to 384 unique dual-index libraries

Sample type purification kit guide 

The 16S V3-V5 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V3-V4 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.

The 16S V3-V5 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V3-V4 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.

Details

Supporting Data

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Minimum amount of starting material:	2.5 µL of DNA (5 ng/µL)
Time to complete library preparation:	4 hours

Storage Conditions and Product Stability
Norgen’s 16S V3-V5Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.

Step	Component	Cat. 70500 (24 preps)	Cat. 70510, 70520, 70530, 70540 (96 preps)
Amplicon PCR (PCR 1)	MGX Master Mix	330 µL	1,320 µL
	16S V3-V5 Primer Mix	70 µL	280 µL
Index PCR (PCR 2)	Indexing Master Mix	660 µL	2 x 1,320 µL
	N7xx Index Primer	50 µL	50 µL
	S5xx Index Primer	70 µL	70 µL
PCR Clean-Up	Resuspension Buffer	2 x 1,250 µL	2 x 5,000 µL
	Nuclease-free water	1,250 µL	1 x 6,000 µL

Description

Specifications

Clone	IHC654
Source	Mouse Monoclonal
Positive Control	Prostate, Prostate Carcinoma
Dilution Range	1:200

Prostate-Specific Antigen (PSA) is a serine protease of the kallikrein family, that is produced by the prostate epithelium and epithelial lining of the periurethral glands. Although considered prostate-specific, PSA has also been detected in breast tissue, breast tumors, endometrium, adrenal neoplasms, and renal cell carcinomas. Anti-PSA can be used for differentiating high-grade prostate adenocarcinoma from high-grade urothelial carcinoma, as well as for determining the prostatic origin of carcinomas in non-prostate tissues. Anti-PSA recognizes primary and metastatic prostatic neoplasms, but not tumors of nonprostatic origin, and can be useful as an aid to confirm prostatic acinar cell origin in primary and metastatic carcinomas.

TDL5E Bench top low speed refrigerated centrifuge machine

Application:

Widely used in the field of biochemistry, biological products, pharmaceutical factory and laboratory.

TDL5E Technical Parameters:

Max. Speed	5000rpm	Max.RCF	4420xg
Max. Volume	4x180ml	Speed Accuracy	±20rpm
Timer	1~99h59min	Noise	≤60dBA
Dimension(LxWxHmm)	570x600x340mm	Net Weight	76Kg
Power Supply	AC220V/110V 50HZ/60HZ	Temperature Range	-20℃~40℃
Temperature Accuracy	±1℃	Certifications	CE, ISO & Calibration report is available.

Matched Rotors for TDL5E:

Order No.	Rotor Type	Max. Speed(rpm)	Volume(ml)	Max.RCF(xg)
5E-1	Swing rotor	4000	4x180ml	2580
5E-2	Microplate rotor	4000	2x3x96well	2020
5E-3	Swing rotor	5000	4x1x50ml	4420
5E-4	Swing rotor	5000	4x1x100ml	4420
5E-5	Swing rotor	4000	4x2x50ml	2820
5E-6	Swing rotor	4000	4x4x15ml	2820
5E-7	Swing rotor	4000	4x6x10/7ml	2580
5E-8	Swing rotor	4000	4x6x5ml	2170
5E-9	Swing rotor	4000	4x4x10/7ml	2580
5E-10	Swing rotor	4000	4x4x5ml	2170
5E-11	Fixed rotor	5000	6x15ml	2540
5E-12	Fixed rotor	5000	12x15ml	3080
5E-13	Fixed rotor	5000	24x15ml	3500
5E-14	Fixed rotor	5000	4x50ml	2520
5E-15	Fixed rotor	5000	6x50ml	2850
5E-16	Fixed rotor	5000	4x100ml	2630
5E-17	Fixed rotor	5000	6x100ml	3130

TDL5E is a table top low speed refrigerated centrifuge widely used in the field of biochemistry, biological products, pharmaceutical factory and laboratory.