12 x 8 strips (96 tests) This ELISA kit is for the quantitative detection of IgG antibodies against parasites of Anisakidae family in human serum. Serology is an aid for diagnosis and cannot be used as the sole method of diagnosis.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Detail
Name of Product
Anisakidae – IgG ELISA
Catalog Number
AF 9800
Short Info
This ELISA kit is for the quantitative detection of IgG antibodies against parasites of Anisakidae family in human serum. Serology is an aid for diagnosis and cannot be used as the sole method of diagnosis.
This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.
Method/Platform
ELISA in microplate format
Range/Assay Sensivity
97%
Test Principle
Specific antibodies in the sample bind to Anisakidae excreted/secreted larval antigens sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A alkaline phosphatase conjugate.
Plasmid DNA is ready for various downstream applications including restriction digestion, bacterial transformation, sequencing and more
Available in 4 formats: MiniPrep, MiniPrep (Magnetic Bead System), 96-Well MiniPrep (Magnetic Bead System), and MaxiPrep
These kits are designed for the rapid preparation of plasmid DNA from Escherichia coli.
Plasmid MiniPrep Kit
This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20 μg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format.
Plasmid MiniPrep Kit (Magnetic Bead System and High Throughput Magnetic Bead System)
Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is designed for the rapid preparation of plasmid DNA from small batch cultures of Escherichia coli. Norgen’s magnetic beads bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. The purified plasmids are fully digestible with all restriction enzymes tested, and are completely compatible with real-time PCR and NGS.
Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is also available in a 96-well (HT) format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Plasmid MaxiPrep Kit
This kit is designed for the rapid spin column preparation of plasmid DNA from up to 100 mL of Escherichia coli cultures. The kit allows for the isolation of plasmid DNA with final endotoxin levels of 0.1 EU/µg of DNA or less. The kit is able to purify plasmids up to 13,000 bp in size, and typical yields from a 100 mL culture for a high copy number plasmid are between 0.4 and 1.0 mg. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with manual or automated sequencing to achieve 95-100% accuracy.
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the of shipment. The RNase should be stored at -20°C upon arrival. The Resuspension Solution AZ should be stored at 4°C upon addition of RNase enzyme.
Component
Cat. 13300 (50 preps)
Cat. 46400 (250 preps)
Cat. 46500 (4 preps)
Cat. 46600 (20 preps)
Cat. 60300 (50 preps)
Cat. 63000 (192 preps)
Resuspension Solution AZ
12 mL
60 mL
20 mL
100 mL
12 mL
2 x 20 mL
Lysis Buffer N
40 mL
80 mL
40 mL
2 x 80 mL
40 mL
2 x 40 mL
Buffer TN
20 mL
130 mL
55 mL
2 x 130 mL
20 mL
1 x 55 mL 1 x 20 mL
Wash Solution E
12 mL
2 x 18 mL
–
–
–
–
Elution Buffer K
8 mL
30 mL
–
–
8 mL
2 x 8 mL
Wash Solution J
–
–
25 mL
3 x 25 mL
–
–
Elution Buffer J
–
–
24 mL
120 mL
–
–
RNase A
1 vial
1 vial
1 vial
1 vial
1 vial
1 vial
Magnetic Bead Suspension
–
–
–
–
1 x 1.1 mL
4 x 1.1 mL
Spin Columns
50
250
–
–
–
–
Collection Tubes
50
250
–
–
–
–
DNA Maxi Spin Columns with Collection Tubes (Clear ring in column)
–
–
4
20
–
–
Maxi Spin Filter Columns with Collection Tubes (Grey ring in column)
Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.
Specifications: 6wells
12wells, 24wells., 48wells, 96wells
PRODUCT FEATURES
The product is made of medical grade USP CLASS VI polymer polystyrene
The product is made under a 100,00- class dust-free manufacturing site
Two kinds of product line up are providing.
For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.
For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.
Gamma radiation sterilization
Non-Pyrogenic, DNase/Rnase free.
Document
Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.
This product supplies a simple and rapid extraction of total RNA from plant and fungal samples. The kit is based on super paramagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction process takes only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure RNA Kits buffers can be used for both manual extraction process and automatic nucleic acid extraction machines. This Kits is suitable for extracting RNA from ≤5×106 cultured cells, 20mg tissue and <50mg plant samples.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from 50mg plant using magnetic particles
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Plant and fungus samples
Sample amount
≤50mg
Yield
2-100μg
Time per run
≤60 minutes
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.
Advantages
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Safe – no phenol chloroform extraction required
Fast – several samples can be extracted in 60 minutes by column method
Universal – two lysates suitable for most plant or fungal tissue samples
Kit Contents
Contents
R664101
R664102
R664103
Purification Times
48 Preps
96 Preps
480 Preps
MagPure RNA Particles
1.7 ml
3.5 ml
18 ml
DNase I
600 μl
2 x 600 μl
10 x 600 μl
DNase Buffer
30 ml
40 ml
200 ml
Buffer PRC1
40 ml
70 ml
350 ml
Buffer RL
40 ml
70 ml
350 ml
Buffer MCB*
18 ml
30 ml
150 ml
Buffer MW1*
22 ml
44 ml
220 ml
Buffer MW2*
20 ml
50 ml
2 x 100 ml
RNase Free Water
10 ml
15 ml
120 ml
Storage and Stability
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.
Document
This product supplies a simple and rapid extraction of total RNA from plant and fungal samples. The kit is based on super paramagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction process takes only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure RNA Kits buffers can be used for both manual extraction process and automatic nucleic acid extraction machines. This Kits is suitable for extracting RNA from ≤5×106 cultured cells, 20mg tissue and
