Product Description

High Sensitivity Amp-Future DNA Isothermal Amplification Kit NFO, -20°C Storage, 48 Tests/Box, ICE Shipping

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).

Technical Parameters:

Parameters	Details
Product Name	DNA Isothermal Amplification Kit NFO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Isothermal nucleic acid Applications

Suitable for DNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

TDL5E Bench top low speed refrigerated centrifuge machine

Application:

Widely used in the field of biochemistry, biological products, pharmaceutical factory and laboratory.

TDL5E Technical Parameters:

Max. Speed	5000rpm	Max.RCF	4420xg
Max. Volume	4x180ml	Speed Accuracy	±20rpm
Timer	1~99h59min	Noise	≤60dBA
Dimension(LxWxHmm)	570x600x340mm	Net Weight	76Kg
Power Supply	AC220V/110V 50HZ/60HZ	Temperature Range	-20℃~40℃
Temperature Accuracy	±1℃	Certifications	CE, ISO & Calibration report is available.

Matched Rotors for TDL5E:

Order No.	Rotor Type	Max. Speed(rpm)	Volume(ml)	Max.RCF(xg)
5E-1	Swing rotor	4000	4x180ml	2580
5E-2	Microplate rotor	4000	2x3x96well	2020
5E-3	Swing rotor	5000	4x1x50ml	4420
5E-4	Swing rotor	5000	4x1x100ml	4420
5E-5	Swing rotor	4000	4x2x50ml	2820
5E-6	Swing rotor	4000	4x4x15ml	2820
5E-7	Swing rotor	4000	4x6x10/7ml	2580
5E-8	Swing rotor	4000	4x6x5ml	2170
5E-9	Swing rotor	4000	4x4x10/7ml	2580
5E-10	Swing rotor	4000	4x4x5ml	2170
5E-11	Fixed rotor	5000	6x15ml	2540
5E-12	Fixed rotor	5000	12x15ml	3080
5E-13	Fixed rotor	5000	24x15ml	3500
5E-14	Fixed rotor	5000	4x50ml	2520
5E-15	Fixed rotor	5000	6x50ml	2850
5E-16	Fixed rotor	5000	4x100ml	2630
5E-17	Fixed rotor	5000	6x100ml	3130

TDL5E is a table top low speed refrigerated centrifuge widely used in the field of biochemistry, biological products, pharmaceutical factory and laboratory.

Introduction

This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.

Details

Specifications

Features	Specifications
Main Functions	Extract viral RNA/DNA from 200μl plasma/serum samples
Applications	RT-PCR，PCR，NGS
Products	Viral total nucleic acid, body cell total nucleic acid, negative bacterial DNA
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Whole blood, plasma, serum, soaking solution and tissue homogenate supernatant
Sample amount	200μl
Yield	2-10μg
Elution volume	≥30μl
Time per run	≤30 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

Advantages

• Fast – several samples can be extracted in 20 minutes by column method
• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Safe – no phenol chloroform extraction required
• Sensitive – DNA/RNA can be recovered at the level of PG
• High yield – carrier RNA contained in the product maximize the recovery of trace nucleic acid

Kit Contents

Contents	IVD4173
Purification Times	100 Preps
HiPure Viral Mini Column	100
2ml Collection Tubes	200
PK/Carrier RNA	50 mg
Protease Dissolve Buffer	5 ml
Buffer AL	30 ml
Buffer MW1	44 ml
Buffer MW2	50 ml
RNase Free Water	15 ml

Storage and Stability

This kit is shipped and stored at room temperature and is valid for 12 months.

Experiment Data

This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.