Antibiotic ELISA Kit Menu

Overview

• Purify sequencing extension products from dye terminators, primers and other contaminants
• Also purify DNA from different enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations.
• High recovery
• Fast and efficient spin column format
• Also available in 96 well format for high throughput

This kit provides a rapid spin column procedure for the purification and clean-up of sequencing and various other enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations. The kit is used to remove reaction contaminants including dye terminators, salts, enzymes, excess primers and primer dimers. Contaminants are undesirable as they can interfere with many downstream applications including sequencing, RFLP, restriction enzyme digestions and ligation. Purification is based on spin-column chromatography without the use of phenol, chloroform or alcohol precipitation. The kit provides a high quality product with up to 90% recovery.

The kit is also available in a 96-well format for high-throughput sequencing reaction clean-up.  Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.

Details

Supporting Data

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Kit Specifications – 96-well
Binding Capacity Per Well	15 μg
Maximum Loading Volume Per Well	500 μL
Average DNA Recovery	Up to 90%
Time to Complete 96 Purifications	20 minutes
Minimum Elution Volume	50 μL

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 34500 (50 preps)	Cat. 34400 (2 x 96-well plate)
Binding Buffer C	30 mL	3 x 30 mL
Wash Solution A	12 mL	2 x 20 mL
Elution Buffer B	8 mL	2 x 15 mL
Spin Columns	50	–
Collection Tubes	50	–
Elution Tubes (1.7 mL)	50	–
96-Well Plate	–	2
Adhesive Tape	–	4
96-Well Collection Plate	–	2
96-Well Elution Plate	–	2
Product Insert	1	1

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request

Permagen’s new low elution bar magnet plate for PCR applications features an individual bar magnet for each row of wells making it the perfect solution for hand pipetting. With its SBS footprint and integrated cushion base, scaling up to the robot is a snap. Available in kit form as well, which includes our X39602 top plate for 0.2 mL tubes & strips  

Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads

SKU #

A012424 (magnet plate only)

A012424KT (Includes top plate kit for 0.2 mL strips and tubes)

Features

• SBS/ SLAS footprint
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• Beads are pulled to tube walls for easy removal
• Integrated cushion base allowing for accidental contact between tips and well bottoms
• Made in USA 
• For Research use only

Compatibility

• Full – Skirt PCR plates (recommended)
• Semi- Skirted PCR plates (manual applications)
• Non- Skirted PCR plates (manual applications)
• 0.2 mL PCR Strips and tubes  (With kit)
• Any magnetic beads
• Any common liquid handlers i.e. Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

Minimum – 5 µL 

Maximum – 140 mL

Permagen’s new low elution bar magnet plate for PCR applications features an individual bar magnet for each row of wells making it the perfect solution for hand pipetting. With its SBS footprint and integrated cushion base, scaling up to the robot is a snap. Available in kit form as well, which includes our X39602 top plate for 0.2 mL tubes & strips