
Description
ELISA kits:
Ampicillin ELISA Kit
Bacitracin ELISA Kit
Benzyl ELISA Kit
Gentamycin ELISA Kit
Kanamycin ELISA Kit
Neomycin ELISA Kit
Penicillin ELISA Kit
Streptomycin ELISA Kit
Highly sensitive, rapid, robust ELISA Kits for screening for Preservative Residue in vaccines
ELISA kits:
Ampicillin ELISA Kit
Bacitracin ELISA Kit
Benzyl ELISA Kit
Gentamycin ELISA Kit
Kanamycin ELISA Kit
Neomycin ELISA Kit
Penicillin ELISA Kit
Streptomycin ELISA Kit
This product supplies a simple and rapid extraction of total RNA from tissue and culture cells samples. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction processis only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure LQ Kit buffers can be used for both manual extraction process and automatic nucleic acid extraction machines.
Specifications
Features | Specifications |
Main Functions | Isolation total RNA from tissue and cells and plant |
Applications | RT-PCR, cDNA synthesis, second generation sequencing |
Purification method | Polydisperse magnetic beads |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Adaptive instrument | Nucleic acid extractor, pipetting workstation |
Sample type | Animal tissue, human tissue, cultured cells, lymphocytes, ordinary plant tissue |
Sample amount | Cells: 1 x 107Animal tissue: ≤20mgPlant tissue: ≤100mgYeast cell: ≤5 x 106 |
Yield | 5-100μg |
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.
Advantages
Kit Contents
Contents | R662201 | R662202 | R662203 |
Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
MagPure RNA Particles | 1.7 ml | 4.0 ml | 18 ml |
Proteinase K | 24 mg | 50 mg | 240 mg |
Protease Dissolve Buffer | 1.8 ml | 5 ml | 15 ml |
DNase I | 600 μl | 2 x 600 μl | 10 x 600 μl |
DNase Buffer | 30 ml | 40 ml | 200 ml |
RTL Lysis Buffer | 40 ml | 80 ml | 400 ml |
Buffer MCB* | 18 ml | 30 ml | 150 ml |
Buffer MW1* | 44 ml | 66 ml | 2 x 220 ml |
Buffer RW2* | 20 ml | 50 ml | 2 x 100 ml |
RNase Free Water | 10 ml | 30 ml | 120 ml |
Storage and Stability
MagPure RNA Particles and Proteinase K should be stored at 2–8°C upon arrival. DNase I shouldbe stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles and Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for atleast 18 months under these conditions.
This product supplies a simple and rapid extraction of total RNA from tissue and culture cells samples. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction processis only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure LQ Kit buffers can be used for both manual extraction process and automatic nucleic acid extraction machines.
These kits are designed for the rapid preparation of plasmid DNA from Escherichia coli.
Plasmid MiniPrep Kit
This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20 μg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format.
Plasmid MiniPrep Kit (Magnetic Bead System and High Throughput Magnetic Bead System)
Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is designed for the rapid preparation of plasmid DNA from small batch cultures of Escherichia coli. Norgen’s magnetic beads bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. The purified plasmids are fully digestible with all restriction enzymes tested, and are completely compatible with real-time PCR and NGS.
Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is also available in a 96-well (HT) format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Plasmid MaxiPrep Kit
This kit is designed for the rapid spin column preparation of plasmid DNA from up to 100 mL of Escherichia coli cultures. The kit allows for the isolation of plasmid DNA with final endotoxin levels of 0.1 EU/µg of DNA or less. The kit is able to purify plasmids up to 13,000 bp in size, and typical yields from a 100 mL culture for a high copy number plasmid are between 0.4 and 1.0 mg. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with manual or automated sequencing to achieve 95-100% accuracy.
Figure 1 / 5
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Kit Specifications | |
Size of Plasmids Purified | Up to 13,000 bp |
Average Yield from 1.5 mL of Culture | Up to 20 μg |
Time to Complete 10 Purifications | 30 minutes (Cat. 60300) 45 minutes (Cat. 63000) |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year from the date of shipment. The RNase should be stored at -20°C upon arrival. The Resuspension Solution A should be stored at 4°C upon addition of RNase enzyme.
Component | Cat. 13300 (50 preps) | Cat. 46400 (250 preps) | Cat. 46500 (4 preps) | Cat. 46600 (20 preps) | Cat. 60300 (50 preps) | Cat. 63000 (192 preps) |
---|---|---|---|---|---|---|
Resuspension Solution AZ | 12 mL | 60 mL | 20 mL | 100 mL | 12 mL | 2 x 20 mL |
Lysis Buffer N | 40 mL | 80 mL | 40 mL | 2 x 80 mL | 40 mL | 2 x 40 mL |
Buffer TN | 20 mL | 130 mL | 55 mL | 2 x 130 mL | 20 mL | 1 x 55 mL 1 x 20 mL |
Wash Solution E | 12 mL | 2 x 18 mL | – | – | – | – |
Elution Buffer K | 8 mL | 30 mL | – | – | 8 mL | 2 x 8 mL |
Wash Solution J | – | – | 25 mL | 3 x 25 mL | – | – |
Elution Buffer J | – | – | 24 mL | 120 mL | – | – |
RNase A | 1 vial | 1 vial | 1 vial | 1 vial | 1 vial | 1 vial |
Magnetic Bead Suspension | – | – | – | – | 1 x 1.1 mL | 4 x 1.1 mL |
Spin Columns | 50 | 250 | – | – | – | – |
Collection Tubes | 50 | 250 | – | – | – | – |
DNA Maxi Spin Columns with Collection Tubes (Clear ring in column) | – | – | 4 | 20 | – | – |
Maxi Spin Filter Columns with Collection Tubes (Grey ring in column) | – | – | 4 | 20 | – | – |
96-Well Plate | – | – | – | – | – | 2 |
Elution Tubes (1.7 mL) | 50 | 250 | – | – | 50 | – |
Elution Tubes (50 mL) | – | – | 4 | 20 | ||
96-Well Elution Plate | – | – | – | – | – | 2 |
Adhesive Tape | – | – | – | – | – | 2 |
Product Insert | 1 | 1 | 1 | 1 | 1 | 1 |
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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