The Available Carbohydrates/Dietary Fiber test kit is an integrated procedure for the measurement and analysis of available carbohydrates and dietary fiber in cereal products, fruit and vegetables and food products.
Detail
K-ACHDF
SKU: 700004259
100 assays of each component
Content:
100 assays of each component
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Available Carbohydrates, Dietary Fiber
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Limit of Detection:
0.5 g/100 g
Reaction Time (min):
~ 78 min
Application examples:
Food ingredients, food products and other materials.
The Available Carbohydrates/Dietary Fiber test kit is an integrated procedure for the measurement and analysis of available carbohydrates and dietary fiber in cereal products, fruit and vegetables and food products.
Gel images of different ranges of library size selection. Sheared human genomic DNA was used as input.
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Library size selection is an enrichment of a specific range of library sizes for NGS library preparations. The NGS library preparation is related to the quality of the sequencing data. Precise NGS library size selection can increase sequencing efficiency, improve data quality, and reduce costs.
There are two types of sequencing technologies: short-read sequencing and long-read sequencing. Short-read sequencing uses DNA libraries that contain small insert DNA fragments of similar sizes, usually several hundred base pairs. The sequencing efficiency can be improved if the DNA size selection is in the right range. Cat.# 20104S and 20104L are the best kits for NGS library size selection of illumina paired-end 100 (PE100) sequencing with 100-200 bp library inserts; Cat.# 20105S and 20105L are the best kits for NGS library size selection of illumina paired-end 150 (PE150) sequencing with 150-300 bp library inserts; and Cat.# 20106S and 20106L are the best kits for NGS library size selection of illumina paired-end 300 (PE300) sequencing with 300-600 bp library inserts.
Long-read sequencing uses a large DNA fragment as input and makes very long reads. Usually, library size selection is preferred to remove smaller fragments. Cat.# 20110S and 20110L are the best kits for long-read sequencing size selection with DNA sizes >5 kb, and Cat.# 20111S and 20111L are the best kits for long-read sequencing size selection with DNA sizes >10 kb.
The magnetic beads, or SPRI (Solid Phase Reversible Immobilization) beads, is well used for the purification of DNA due to their reversible DNA binding. The NGS library can be size-selected by the magnetic beads or SPRI beads. The properties of the magnetic beads can be changed for a specific range of DNA binding. The contaminants and other unwanted components in the libraries can also be removed during size selection.
Specific ranges of NGS libraries can be selected using magnetic beads with different buffer compositions. The first DNA-beads binding step, also called the right-side clean-up, removes large NGS library fragments. The large NGS library fragments that bind to the beads are discarded with the beads pellet. The desired NGS library fragments in the supernatant are transferred to a new well, and new beads are added to the supernatant for the second beads-DNA binding, also called the left-side clean-up. After the rinsing step, the NGS library fragments with the dual selection are eluted in water or an appropriate buffer. The magnetic beads method has great advantages over time-consuming column purification and tedious gel-based purification.
NGS library size selection with dual clean-ups.
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Library size selection for long-read sequencing only requires a single clean-up. In this case, only the large library fragments are bound to the beads, while other small library fragments are discarded with the supernatant. The selected larger library fragments are eluted in water or an appropriate buffer after the rinsing step.
NGS library size selection with single clean-up for >5 kb and >10 kb libraries.
Solid Phase Reversible Immobilization magnetic beads are often used for DNA purification because they are simple, fast, and effective. The beads are paramagnetic particles coated with carboxyl groups that reversibly bind to nucleic acid. However, Standard SPRI beads can only purify DNA/RNA fragments that are 100 base pairs or longer. DNA/RNA fragments shorter than 100 base pairs are not effectively recovered by SPRI beads. We have developed Magnetic Beads(microRNA & Oligo Purification) to solve the problem.
With our proprietary beads technology, the beads overcomes the hurdle of the short DNA/RNA recovery problem. The magnetic beads is ideal for microRNA purification, oligo purification, short DNA/RNA purification, and removing impurities and unwanted components such as dNTPs, detergents, salts, proteins, and other contaminants effectively. The magnetic bead reagents are RNase-free and can be used for both DNA and RNA applications.
Our magnetic beads are optimized for microRNA purification, oligo purification, and DNA/RNA purification. The fragments can be as short as 20 bases, such as microRNA, tRNA, dsDNA fragments 20 bp or longer, ssDNA fragments 20 nt or longer, RNA fragments 20 nt or longer, DNA/RNA hybrid fragments 20 bp or longer, and oligos and chimeric oligos 20 nt or longer. Purified short DNA and RNA fragments are ideal for applications requiring high-quality fragments, as the fragments are free of impurities and contaminants.
Comparison of short DNA fragments recovery. BioDynami 20 bp DNA ladder (Cat. # 10002) was used as DNA input. Ampure XP beads, BioDynami beads (DNA & RNA purification) and BioDynami beads (microRNA & Oligo purification) were tested. Only BioDynami beads (microRNA & Oligo purification) successfully recovered DNA fragments between 20-100 bp.
Recovery of DNA and RNA oligos with BioDynami magnetic Beads (microRNA & Oligo Purification). 20 nt of DNA oligos and RNA oligos were used as input. Input and recovered oligos were quantified with BioDynami ssDNA Quantification kit (Cat. # 40043) and BioDynami RNA Quantification kit (Cat. # 40044).
Features
Effective purification of short DNA and RNA samples
microRNA
tRNA
dsDNA fragments 20 bp or longer
ssDNA fragments 20 nt or longer
RNA fragments 20 nt or longer
DNA/RNA hybrid fragments 20 bp or longer
Oligo and chimeric oligo 20 nt or longer
Removal of impurities and unwanted reaction components
Compatibility: Works with both manual and automated procedures
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Document
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
150 reactions
