Biotin-PEG8-DBCO

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request

Overview

• Convenient ready-to-use solution
• High sensitivity and yield
• Robust amplification

Norgen’s 2X PCR Master Mix is a ready-to-use solution that contains components required for PCR amplification including Taq DNA polymerase, dNTPs, reaction buffer, MgCl₂, KCl and a PCR enhancer/stabilizer. The user needs only to add template, the primer set and water to the master mix in order to set up the PCR reaction. This convenient 2X PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of DNA templates with high yields of PCR products.

Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with Norgen’s 2X PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1.

Details

Supporting Data

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Reagents Supplied – Cat # 28007

•  2X PCR Master Mix (1 Vial, 100 Reactions) – Sufficient reagent for 100 x 20 µL reactions

Storage Conditions and Product Stability
Norgen’s 2X Master Mix should be stored at -20ºC. For everyday use an aliquot can be stored at 4ºC for up to three months. The Master Mix is stable for multiple freeze-thaw cycles (see Figure 2). When stored at the proper temperature this reagent is stable for at least 1 year.

t-Boc-N-Amido-PEG12-propargyl can participate in copper catalyzed Click Chemistry reactions with biomolecules that contain azide. The t-Boc protected amine can be deprotected under mild acidic conditions. The PEG spacer helps improve the water-solubility of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

t-Boc-N-Amido-PEG12-propargyl can participate in copper catalyzed Click Chemistry reactions with biomolecules that contain azide. The t-Boc protected amine can be deprotected under mild acidic conditions. The PEG spacer helps improve the water-solubility of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.