Bis-propargyl-PEG3 is a crosslinker with alkyne groups at both ends of the molecule. The alkyne groups react with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Bis-propargyl-PEG3 is a crosslinker with alkyne groups at both ends of the molecule. The alkyne groups react with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Attogene’s Carbon Dioxide Enzymatic Assay Kit is a simple, direct method for measuring Carbon Dioxide levels in the environment. The assay uses a coupled enzyme assay to detect CO2 (as HCO3-) as follows. In the first step, the bicarbonate condenses with phosphoenol pyruvate to form oxalate (and phosphoric acid); this reaction is catalyzed by the enzyme Phosphoenolpyruvate Decarboxylase, PEPC. The oxalate is then enzymatically reduced by the enzyme Malate Dehydrogenase (using an NADH cofactor) to form malate and NAD+.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Phytase
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
360
Signal Response:
Increase
Linear Range:
0.1 to 10 μg of phosphate per assay
Limit of Detection:
1.5 U/L
Reproducibility (%):
< 7%
Reaction Time (min):
~ 30 min
Application examples:
Animal feeds, phytase activity in cereal, fungal and bacterial phytases.
Method recognition:
Novel method
The Phytase Assay Kit is a simple, quantitative method which can be used to measure phytase activity. Analysis is based on the hydrolysis of phytic acid by phytase and quantitative measurement of the phosphate released. Results are measured using a standard UV-VIS spectrophotometer and do not require the creation of a standard curve. The Phytase method can be used to measure phytase activity in cereal, fungal and bacterial phytases, and can also be used for the analysis of phytase in animal feed samples.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Glycerol
Assay Format:
Spectrophotometer, Microplate, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
1.0 to 35 µg of glycerol per assay
Limit of Detection:
0.37 mg/L
Reaction Time (min):
~ 7 min
Application examples:
Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices, soft drinks, toothpaste, honey, tobacco, paper (and cardboard), cosmetics, pharmaceuticals, soap and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Novel method
The Glycerol GK test kit is a simple, reliable and accurate method for the measurement and analysis of glycerol in beverages, foodstuffs and other material. Based on use of ADP-glucokinase and increase in absorbance on conversion of NAD+ to NADH.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Novel tablet format for increased stability
Very competitive price (cost per test)
All reagents stable for > 2 years as supplied
Very rapid reaction
Positive reaction (assay proceeds with an increase in absorbance)
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats
Document
The Glycerol GK test kit is a simple, reliable and accurate method for the measurement and analysis of glycerol in beverages, foodstuffs and other material. Based on use of ADP-glucokinase and increase in absorbance on conversion of NAD+ to NADH.