
Description
- Appearance: White/Pink with lens
- • Length of strip: 86mm
- • Width of strip: 6mm
- • Length of device: 140mm
- • Width of device: 15mm
500 Lateral Flow Cassettes (top and bottom)
Cassette Lettering: CT
Fits strips that are 86 mm x 6 mm
Perfect for development and product launch
Large quantities available
These kits provide a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from mammalian blood samples. These kits are supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. These kits are able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.
Leukocyte RNA Purification Kit (Spin Column)
This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. Allowable blood input ranges from 10 μL to 2 mL or 3 x 106 Leukocytes
Leukocyte RNA Purification Plus Kit (Plus)
Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. Complete 10 purifications in as little as 40 minutes.
Leukocyte RNA Purification 96-Well Kit (High Throughput)
Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Norgen’s kit allows for the isolation of total leukocyte RNA, including all small RNA species. The purified RNA is of the highest quality and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension, and expression array assays. Allowable blood input ranges from 10 μL to 1 mL or 1.5 x 106 Leukocytes.
Figure 1 / 6
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Kit Specifications | |
Maximum Binding Capacity Per Well | 50 μg |
Maximum Loading Volume Per Well | 500 μL |
Size of RNA Purified | All sizes, including small RNA < 200 nt |
Maximum Blood Input* | 1 mL or 1.5 x 106 Leukocytes |
Standard Blood Input | 150 μL |
Minimum Blood Input | 10 μL |
Average Yield: 500 μL human blood | 1.5 μg |
Time to Complete 10 Purification | 40 minutes |
*Additional RBC Lysis Solution is required for input Volumes >150 µL
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.
Component | Cat. 21200 (50 preps) | Cat. 21250 (50 preps) | Cat. 37800 (2 x 96 preps) |
---|---|---|---|
RBC Lysis Buffer | 2 x 100 mL | 2 x 1 L | 2 x 90 mL |
Buffer RL | 30 mL | 30 mL | – |
Binding Solution | – | – | 2 x 40 mL |
Wash Solution A | 38 mL | 38 mL | – |
Wash Solution | – | – | 2 x 30 mL |
Elution Solution A | 6 mL | 6 mL | – |
Elution Solution | – | – | 2 x 20 mL |
Enzyme Incubation Buffer | – | 6 mL | 1 |
DNase I | – | 1 Vial | – |
Mini Spin Columns | 50 | – | – |
Lysate Homogenization Column | – | 50 | – |
Single Cell RNA Column | – | 50 | – |
RBC Lysis 96-Well Plate | – | – | 2 |
96-Well Filter Plate | – | – | 2 |
Adhesive Tape | – | – | 4 |
Collection Tubes | 50 | 100 | – |
96-Well Collection Plate | – | – | 2 |
Elution Tubes (1.7 mL) | 50 | 50 | – |
96-Well Elution Plate | – | – | 2 |
Product Insert | 1 | 1 | 1 |
Name of Product
HIT – Heparin-Induced Thrombocytopenia Test (5)
Catalog Number
MQHIT Z
Short Info
Lateral Flow Assay designed for the detection of IgG antibodies against PF4/polyanion-complexes in human citrated plasma or serum.
This product is only available in Germany!
Method/Platform
lateral flow, immunoassay
Range/Assay Sensivity
Test Principle
IgG antibodies are resposible for the heparin induced thrombocytopenia.
Immobilized anti-human IgG on the membrane of the test unit binds patients IgG-antibodies which are previously captured by the PF4/polyanion-complex which is detected by intensely colored gold nanoparticles.
The presence of PF4/polyanion-complex becomes visible at a colored test line. The surplus of gold particles continues to migrate through the membrane and is captured at the control line by specific antibodies.
Lateral Flow Assay designed for the detection of IgG antibodies against PF4/polyanion-complexes in human citrated plasma or serum.
Herpes Simplex Virus 2 (HSV-2) is a member of the herpes virus family, Herpesviridae. HSV-2 has a relatively large double-stranded DNA genome. HSVs are primarily transmitted by sexual intercourse, direct contact with lesions or perinatally. Most HSV positive cases are characterised by lesions on the skins and mucous membranes of the mouth and genitals. HSV infection can be either primary or a recurrence of a previous infection. More than 90% of the primary HSV infections are asymptomatic. Primary infection with HSV-1 can lead to gingivostomatitis, eczema herpeticum, keratoconjunctivitis and encephalitis. The primary symptoms of a secondary infection are skin lesions in the nose, mouth and genital regions. The infection is contagious, mainly during an epidemic.
HSV-2 TaqMan PCR Kit, 100 reactions
HSV-2 TaqMan PCR Probe/Primer Set and Controls, 100 reactions
Figure 1 / 3
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component | Cat. TM32450 (100 preps) | Cat. TM32410 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
HSV-2 Primer & Probe Mix | 280 μL | 280 μL |
HSV-2 Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |
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