Introduction
Place of Origin: Guangdong, China
Warranty: 2 years
Customized support: OEM
Brand Name: HKM
Model Number: CRM008
Reagent grade: Biochemical Reagent
Form: Powder
Color: Light yellow
Type: Vibro Agar
1000mL
Place of Origin: Guangdong, China
Warranty: 2 years
Customized support: OEM
Brand Name: HKM
Model Number: CRM008
Reagent grade: Biochemical Reagent
Form: Powder
Color: Light yellow
Type: Vibro Agar
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
The series of nucleic acid columns produced by Magen Biotech are based on carefully selected imported glass fiber membranes (GF/B, GF/D, GF/F). Columns production processes such as polypropylene injection molding materials, injection molding process, and downstream membrane packing and compression rings are strictly controlled. This is to ensure that the column has extremely high adsorption capacity and long-term stability. Compared with conventional products on the market, Magen’s columns are with varieties, and binding rate will not change when stored at room temperature for 4 years.
Specifications
| Features | Specifications |
| Recommended application | gDNA maxi yield preparation, total RNA maxi preparation |
| Preservation conditions | Room temperature |
| Stability | Up to 4 years |
| Filter membrane | High quality glass fiber filter GF/B, 4 layers |
| Membrane aperture | 1.0μm |
| Maximum binding yield of plasmid | 500 μg |
| Maximum yield of alcohol mediated binding | 5 mg |
| gDNA or RNA yield (>30%ethanol) | Up to 5mg |
| Single liquid carrying capacity of column | 20 ml |
| Minimum elution volume | 800 μl |
| Withstand centrifugal force | 3,000~5,000 x g |
| Centrifuge | Low speed centrifuge for 50mlcentrifuge tubes, >3000 x g, swing-out Rotor |
Adsorption Mechanism
Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silica gel membrane, while other pollutants, mainly proteins, are removed by membrane washing.
Ordering information
| CAT.No. | Product Name | Package |
| C13122 | HiPure gDNA Maxi Column (4 x GF/B)with 50ml Collection Tubes | 100/Bag |
| Item No. | Product Name | Membrane type/number of layers | Collection tubes | Plasmid DNA binding capacity (Physical adsorption) | gDNA/RNA binding capacity (Alcohol-mediated adsorption) | Minimum Elution volume | Liquid volume capacity |
| C13010 | HiPure DNA Nano Column | 2 layers GF/F | 2ml without cap | 5μg | 20μg | 10μl | 700μl |
| C13011 | HiPure DNA Micro Column | 3 layers GF/F | 2ml without cap | 10μg | 50μg | 15μl | 700μl |
| C13100 | HiPure DNA Mini Column I | 2 layers GF/B | 2ml without cap | 15μg | 100μg | 30μl | 700μl |
| C13110 | HiPure DNA Mini Column II | 4 layers GF/B | 2ml without cap | 35μg | 200μg | 50μl | 800μl |
| C13111 | HiPure RNA Mini Column | 3 layers GF/B | 2ml without cap | 30μg | 200μg | 30μl | 800μl |
| C13112 | HiPure Viral Mini Column | 3 layers GF/F | 2ml without cap | 30μg | 200μg | 30μl | 800μl |
| C13113 | HiPure CFDNA Mini Column | 3 layers GF/F,1 layer GF/B | 2ml without cap | 30μg | 200μg | 30μl | 800μl |
| C13120 | HiPure DNA Midi Column | 4 layers GF/B | 15ml Centrifuge tube | 125μg | 1mg | 500μl | 4ml |
| C13121 | HiPure DNA Midi Column III | 8 layers GF/B | 15ml Centrifuge tube | 250μg | 1mg | 500μl | 4ml |
| C13122 | HiPure DNA Maxi Column | 4 layers GF/B | 50ml Centrifuge tube | 500μg | 5mg | 1000μl | 20ml |
| C13123 | HiPure DNA Maxi Column III | 8 layers GF/B | 50ml Centrifuge tube | 1mg | 5mg | 1000μl | 20ml |
| C13124 | HiPure DNA Maxi Column C | 8 layers GF/B | 50ml high speed Centrifuge tube | 1mg | 5mg | 700μl | 12ml |
| C13130 | HiPure DNA Plate | 2 layers GF/F | 1.6ml Plate | 30μg | 100μg | 80μl | 900μl |
| C13131 | HiPure gDNA Plate | 2 layers GF/B | 1.6ml Plate | 30μg | 100μg | 80μl | 900μl |
Note: GF/B pore size is for 1.0μM glass fiber membrane; GF/F pore size is for 0.7μm glass fiber membrane.
Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.
TD5A Low Speed Centrifuge
Features:
1. Microprocessor control, digital display, touch panel, parameters in running can be edited and change to display other running parameter and RCF.
2. Brushless frequency motor with simpler construction, more reliable performance, longer life and quietly running.
3. Automatic lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.
4. Adapters are available by experiment requirements.
5. 3 tiers protection steel cover, safe and reliable.
6. TUV CE EMC certificate.
TD5A Technical Parameter：
Matched Rotor for TD5A
| Order NO. | Rotor Type | Max speed(r/min) | Volume (ml) | Max RCF(*g) |
| 5A-1 | Swing rotor (round cup) | 4000 | 4×300ml | 3180 |
| 5A-2 | Swing rotor (square cup) | 4000 | 4×300ml | 3150 |
| 5A-3 | Swing rotor | 4000 | 4×24×7ml | 3150 |
| 4000 | 4×24×5ml | 2750 | ||
| 4000 | 4×18×10ml | 3140 | ||
| 5A-4 | Microplate rotor | 4000 | 2×4×96 well | 2910 |
| 5A-5 | Microplate rotor | 4000 | 2×4×48 well | 2300 |
| 5A-6 | Swing rotor | 5000 | 4×1×50ml | 4730 |
| 5000 | 4×1×100ml | 4730 | ||
| 5A-7 | Swing rotor | 4000 | 4×2×50ml | 3200 |
| 4000 | 4×2×100ml | 3020 | ||
| 4000 | 4×4×15ml | 3200 | ||
| 4000 | 4×6×15ml | 3200 | ||
| 4000 | 4×8×15ml | 3200 | ||
| 4000 | 4×12×5ml vacuum tube | 2480 | ||
| 4000 | 4×12×7ml vacuum tube | 2760 | ||
| 4000 | 4×12×10ml vacuum tube | 2790 | ||
| 4000 | 4×8×10/7/5ml vacuum tube | 2790 | ||
| 4000 | 4×6×10/7/5ml vacuum tube | 2790 | ||
| 4000 | 4×4×10/7/5ml vacuum tube | 2790 | ||
| 5A-8 | Fixed Rotor | 5000 | 6×15ml | 2540 |
| 5A-9 | Fixed Rotor | 5000 | 12×15ml | 3080 |
| 5A-10 | Fixed Rotor | 5000 | 24×15ml | 3500 |
| 5A-11 | Fixed Rotor | 5000 | 30×15ml | 3830 |
| 5A-12 | Fixed Rotor | 5000 | 4×50ml | 2520 |
| 5A-13 | Fixed Rotor | 5000 | 6×50ml | 2850 |
| 5A-14 | Fixed Rotor | 5000 | 12×50ml | 3860 |
| 5A-15 | Fixed Rotor | 4000 | 24×50ml | 2970 |
| 5A-16 | Fixed Rotor | 5000 | 4×100ml | 2630 |
| 5A-17 | Fixed Rotor | 5000 | 6×100ml | 3130 |
| 5A-18 | Fixed Rotor | 4000 | 12×100ml | 2970 |
TD5A centrifuge Max Capacity 4x300ml swing out rotor, max speed 5000rpm, can hold 96 vacuum tubes
