CRM008 Chromogenic Vibro Agar

Overview

• CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
• Input volumes up to 200 µL
• High yield and high quality DNA ready for any application
• Fast and convenient spin column protocol

Norgen’s Blood Genomic DNA Isolation Mini Kit Dx is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood for subsequent in vitro diagnostic use. Both fresh and frozen anticoagulated blood may be used with this procedure. Purification is based on spin column chromatography as the separation matrix. Norgen’s column binds DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions.

This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of DNA followed by signal detection or amplification. Any diagnostic results generated using the DNA isolated with Norgen’s Blood Genomic DNA Isolation Mini Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.

To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.

Norgen’s Blood Genomic DNA Isolation Mini Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques including experience with whole blood samples and DNA isolation.

Norgen’s Blood Genomic DNA Isolation Mini Kit Dx does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.

Details

Supporting Data

Figure 1 / 3

Previous

Next

Click for expanded view

Kit Specifications
Minimum Blood Input	20 µL
Maximum Blood Input	200 µL
Column Binding Capacity	> 50 µg
Average Yield (200 µL of blood)	4-12 µg*
Time to Complete 10 Purifications	30 minutes

*Yield will vary depending on the type of blood processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers. The kit contains a ready-to-use Proteinase K solution, which is dissolved in a specially prepared storage buffer. The Proteinase K is stable for up to 2 years after delivery when stored at room temperature. To prolong the lifetime of Proteinase K, storage at 2–8°C is recommended.

Component	Cat. Dx46300 (50 preps)
Lysis Solution	20 mL
Wash Solution I	18 mL
Wash Solution II	18 mL
Elution Buffer	12 mL
Proteinase K	1.2 mL
Spin Columns	50
Collection Tubes	50
Elution Tubes	50
Product Insert	1

Overview

• Fractionate leukocytes from whole blood in minutes with provided RBC Lysis Buffer
• Isolate total RNA, including microRNA, without phenol
• Rapid and convenient spin-column format and 96-well plate for high throughput applications
• Purified RNA is ready for any downstream application including RT-PCR, qRT-PCR, NGS, arrays and more. Find out more information on Norgen’s NGS services
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits provide a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from mammalian blood samples. These kits are supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. These kits are able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.

Leukocyte RNA Purification Kit (Spin Column)

This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. Allowable blood input ranges from 10 μL to 2 mL or 3 x 10⁶ Leukocytes

Leukocyte RNA Purification Plus Kit (Plus)

Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. Complete 10 purifications in as little as 40 minutes.

Leukocyte RNA Purification 96-Well Kit (High Throughput)

Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Norgen’s kit allows for the isolation of total leukocyte RNA, including all small RNA species. The purified RNA is of the highest quality and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension, and expression array assays. Allowable blood input ranges from 10 μL to 1 mL or 1.5 x 10⁶ Leukocytes.

Details

Supporting Data

Figure 1 / 6

Previous

Next

Click for expanded view

Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of RNA Purified	All sizes, including small RNA < 200 nt
Maximum Blood Input*	1 mL or 1.5 x 106 Leukocytes
Standard Blood Input	150 μL
Minimum Blood Input	10 μL
Average Yield: 500 μL human blood	1.5 μg
Time to Complete 10 Purification	40 minutes

*Additional RBC Lysis Solution is required for input Volumes >150 µL

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  The RBC Lysis Buffer should be stored at 4°C upon arrival.  These reagents should remain stable for at least 1 year in their unopened containers.

Component	Cat. 21200 (50 preps)	Cat. 21250 (50 preps)	Cat. 37800 (2 x 96 preps)
RBC Lysis Buffer	2 x 100 mL	2 x 1 L	2 x 90 mL
Buffer RL	30 mL	30 mL	–
Binding Solution	–	–	2 x 40 mL
Wash Solution A	38 mL	38 mL	–
Wash Solution	–	–	2 x 30 mL
Elution Solution A	6 mL	6 mL	–
Elution Solution	–	–	2 x 20 mL
Enzyme Incubation Buffer	–	6 mL	1
DNase I	–	1 Vial	–
Mini Spin Columns	50	–	–
Lysate Homogenization Column	–	50	–
Single Cell RNA Column	–	50	–
RBC Lysis 96-Well Plate	–	–	2
96-Well Filter Plate	–	–	2
Adhesive Tape	–	–	4
Collection Tubes	50	100	–
96-Well Collection Plate	–	–	2
Elution Tubes (1.7 mL)	50	50	–
96-Well Elution Plate	–	–	2
Product Insert	1	1	1