D-Glucose Assay Kit (GOPOD Format)

Overview

• Sample collection is non-invasive and painless
• Fast and easy high throughput processing using 96-well plates
• Isolate high quality genomic DNA
• Effective removal of PCR inhibitors
• Compatible with preserved saliva samples collected using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples

This kit features a rapid, high-throughput 96-well format for isolating high yield and high quality DNA from saliva.  The purified saliva DNA is compatible with any number of downstream applications including PCR, qPCR, Southern Blot analysis, sequencing, microarray analysis and more. This kit is also compatible with Norgen’s Saliva DNA Collection and Preservation Devices (Cat. RU49000).  It is fully compatible to both vacuum and centrifugation protocols.

​Background

Human genomic DNA found in saliva can be used in various applications in diagnostics including the detection of biomarkers to diagnose a disease, follow the disease’s progress or monitor the effects of a particular treatment. Isolation of DNA from saliva for diagnostics has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive.

Details

Supporting Data

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Kit Specifications
Binding Capactity Per Well	50 μg
Maximum Loading Volume Per Well	600 μL
Size of DNA Purified	All sizes
Time to Complete 96 Purifications	40 minutes

Storage Conditions and Product Stability
The kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at room temperature or 4°C. All other solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.

Component	Cat. RU35200 (192 preps)
Lysis Buffer F	100 mL
Proteinase K in Storage Buffer	4 mL
Binding Buffer B	40 mL
Wash Solution A	2 x 38 mL
Elution Buffer B	30 mL
96-Well Plate	2
96-Well Collection Plate	2
Adhesive Tape	4
96-Well Elution Plate	2
Product Insert	1

The Opentrons Flex Gripper GEN1 attaches to the gantry and is capable of moving labware across the deck and on or off modules such as the Magnetic Block or Thermocycler. It can also access the additional slots to the side of the main deck to replenish tips or labware. The gripper can be used with any pipette configuration.

Included with the gripper is a calibration pin that enables automatic calibration to ensure positional accuracy. Currently, the gripper is optimized for use with the following labware items:

– NEST 2 mL 96 Deep Well Plate, V bottom
– Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt
– NEST 96 Well Plate Flat
– All Opentrons Flex tip racks

The Opentrons Flex Gripper GEN1 attaches to the gantry and is capable of moving labware across the deck and on or off modules such as the Magnetic Block or Thermocycler. It can also access the additional slots to the side of the main deck to replenish tips or labware. The gripper can be used with any pipette configuration.

Included with the gripper is a calibration pin that enables automatic calibration to ensure positional accuracy. Currently, the gripper is optimized for use with the following labware items:

– NEST 2 mL 96 Deep Well Plate, V bottom
– Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt
– NEST 96 Well Plate Flat
– All Opentrons Flex tip racks

Overview

• No need for staining and destaining of denaturing gels since Norgen’s loading buffer contains ethidium bromide
• Highly stable
• Nine bands from 200 bases to 4000 bases
• Convenient lyophilized format provides better product stability

The Norgen 1 kb RNA Ladder is a set of RNA transcripts derived from recombinant DNA templates. This ladder is suitable for precise sizing of a wide range of RNA molecules using native or denaturing agarose gels, and can be visualized under UV.

Contents: 
• 2 vials of lyophilized RNA ladder (25 loads each)
• 1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution
• 2x loading buffer

Details

Supporting Data

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Specifications:
• Nine discrete bands, ranging from 200 to 4,000 bases
• Higher intensity band at 1,000 bases for easy reference

Contents:
• 2 vials of lyophilized RNA ladder (25 loads each)
• 1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution
• 2x loading buffer

Instructions:
To reconstitute the lyophilized RNA ladder, add 250 µL of 1x loading buffer to each 25 loads vial and vortex gently.
Heat at 80°C for 10 minutes. Incubate on ice for 1 min. Load 10 µL on a 1-2% gel. For optimal results, use Norgen 2x loading buffer with each RNA sample. No need for staining and destaining of denaturing gels since Norgen’s loading buffer contains ethidium bromide.

Store at -20°C.

Documentation