This product provides fast and easy method for purification of high purity DNA from bone samples. The obtained DNA can be directly used for PCR and STR detection down stream application.
Detail
Introduction
This product provides fast and easy method for purification of high purity DNA from bone samples. The obtained DNA can be directly used for PCR and STR detection down stream application.
Details
Kit Contents
Contents
D312402
D312403
Purification Times
50
250
HiPure DNA Mini Columns I
50
250
2ml Collection Tubes
50
250
Buffer BGL
40 ml
180 ml
Buffer GXP
30 ml
150 ml
Buffer GW1*
13 ml
53 ml
Buffer GW2*
20 ml
2 x 50 ml
DTT Powder
235 mg
2 x 235 mg
Proteinase K
48 mg
240 mg
Protease Dissolve Buffer
5 ml
15 ml
Elution Buffer
15 ml
60 ml
Storage and Stability
This product can be stored at room temperature (15~25°C) for 18 months. Proteinase K/DTT dry powder can be transported and stored at room temperature. For long-term storage (>6 months), it is recommended to store at -20~8°C. Dissolved Proteinase K should be stored at -20~8°C. The dissolved DTT should be stored at -20°C.
Other Products
Thermocycler GEN2 Seals (10 count)
Product Info
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Product Info
Opentrons Thermocycler GEN2 Seals are adhesive-backed Ethylene Propylene Diene Monomer (EPDM) seals designed for use with the fully-automated Thermocycler Module GEN2. They come in a package of 10 seals. These seals need to be applied to the hood of the Thermocycler and can be reused up to 20 times. Opentrons Thermocycler GEN2 Seals need to be sterilized before each use.
Opentrons Thermocycler GEN2 Seals are adhesive-backed Ethylene Propylene Diene Monomer (EPDM) seals designed for use with the fully-automated Thermocycler Module GEN2. They come in a package of 10 seals. These seals need to be applied to the hood of the Thermocycler and can be reused up to 20 times. Opentrons Thermocycler GEN2 Seals need to be sterilized before each use.
Note, if you have a Thermocycler Module GEN1, please use the Thermocycler GEN1 Seals.
DBCO-NHCO-PEG13-NHS ester is a long chain PEG linker containing NHS ester that is able to react specifically and efficiently with primary amines to form astable amide bond. The hydrophilic PEG13 arm improves water solubility and can also provide a long and flexible connection that minimizes steric hindrance during ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
DBCO-NHCO-PEG13-NHS ester is a long chain PEG linker containing NHS ester that is able to react specifically and efficiently with primary amines to form astable amide bond. The hydrophilic PEG13 arm improves water solubility and can also provide a long and flexible connection that minimizes steric hindrance during ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
[CK1000] Champion™ E. coli Transformation Kit, 200 Rxn
Product Info
Document
Product Info
Description
Champion™ E. coli Transformation Kit provides an easy method for rapid preparation of chemically competent cells with high transformation efficiency from fresh culture, overnight culture, or even directly from bacterial colonies on the plate. The competent cell preparation method eliminates the requirement of time-wasting wash step. In addition, preparation of competent cells from overnight culture or directly from bacterial colonies provides flexibility to cloning experiment. The resultant competent cells can be immediately used or stored at -70°C for one year. This kit includes a specialized SMO-Broth™ medium and a unique Champion™ CC Buffer for culturing and preparing competent cells efficiently. Following the simple and quick competent cell preparation protocol from fresh culture, the transformation efficiency is typically ranged from 108–109 cfu/μg transformants/μg of pUC19 plasmid DNA, but varies depending on the E. coli strains. The resultant competent cells can be further transformed using time-saving transformation protocol, eliminating the requirement of heat-shock and recovery steps.
Features
Flexible– fresh culture, overnight culture, 4°C stored liquid culture or even colonies on agar plate can be used for transformation.
Fast and Easy– only few steps for preparation; suitable for time-saving transformation
High efficiency– up to 109 cfu/μg
Personalization– suitable for most E. coli strains
Kit Contents
Component
Volume
Champion™ CC Buffer
20 ml
SMO-Broth™
100 ml x 2
pUC19 Control Plasmid (10-4 μg/μl)
5 µl
Instruction Manual
1
Champion™ Competent Cell Preparation Card
1
Storage
4°C for 12 months
Document
Champion™ E. coli Transformation Kit provides an easy method for rapid preparation of chemically competent cells with high transformation efficiency from fresh culture, overnight culture, or even directly from bacterial colonies on the plate. The competent cell preparation method eliminates the requirement of time-wasting wash step. In addition, preparation of competent cells from overnight culture or directly from bacterial colonies provides flexibility to cloning experiment. The resultant competent cells can be immediately used or stored at -70°C for one year. This kit includes a specialized SMO-Broth™ medium and a unique Champion™ CC Buffer for culturing and preparing competent cells efficiently. Following the simple and quick competent cell preparation protocol from fresh culture, the transformation efficiency is typically ranged from 108–109 cfu/μg transformants/μg of pUC19 plasmid DNA, but varies depending on the E. coli strains. The resultant competent cells can be further transformed using time-saving transformation protocol, eliminating the requirement of heat-shock and recovery steps.
