DBCO-PEG8-PFP ester

Overview

• Genomic DNA can be isolated from as few as 10 bacterial cells in 1 mL of milk
• Isolate genomic DNA from both Gram-negative and Gram-positive bacteria in milk
• Can process challenging samples such as mastitic milk
• Inhibitor-free DNA is ready for PCR, qPCR, Southern Blot, sequencing & more
• Fast and efficient spin-column format

This kit provides a rapid spin column method for the isolation and purification of genomic DNA from both Gram-negative and Gram-positive bacteria in milk samples.  It can also be used to process challenging milk samples such as Mastitic milk for example.  The kit is highly sensitive and can isolate DNA from a cell density of as little as 10 bacteria contained in 1 mL of milk.  Genomic DNA is isolated in 45 minutes, free of inhibitors and ready for any number of downstream applications including PCR, qPCR and Southern Blot analysis, sequencing and more.​

Details

Supporting Data

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Kit Specifications
Maximum Milk Input	1 mL
Time to Complete 10 Purifications	1 hour
DNA Yield*	500 ng to 8 μg
Bacteria Species Processed	Gram positive and Gram negative
Minimum Detection Limit	10 bacteria in 1 mL of milk

*The range of the DNA yield will vary depending upon a number of factors including bacterial species and type of milk (fat content and %)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Lysozyme should be stored at -20°C upon arrival, and the Resuspension Solution A should be stored at -20°C after addition of the lysozyme. The lyophilized Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year from the date of shipment.

Component	Cat. 21550 (50 preps)
Resuspension Solution A	6 mL
Buffer SK	60 mL
Wash Solution A	18 mL
Elution Buffer B	15 mL
Proteinase K	12 mg
Lysozyme (powder)	120 mg
Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Description

The Primerdesign™ genesig® kit for Dengue subtypes genomes is designed for the in vitro
differentiation of Dengue subtypes (Dengue-1, Dengue-2, Dengue-3, and Dengue-4). The kit is designed to have the broadest detection profile possible whilst remaining specific to the individual Dengue subtypes.

The primers and probe sequences in this kit have 100% homology with a broad range of clinically relevant reference sequences based on a comprehensive bioinformatics analysis. They therefore have a very broad quantification profile.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings 150 reactions

DBCO-PEG24-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG24-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.