endo-BCN-PEG4-PFP ester is a click chemistry reagent containing a PFP ester and a BCN group. PFP esters have similar applications as the NHS esters, but are more stable in aqueous solution. They can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group cais reactive with azide-tagged molecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
endo-BCN-PEG4-PFP ester is a click chemistry reagent containing a PFP ester and a BCN group. PFP esters have similar applications as the NHS esters, but are more stable in aqueous solution. They can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group cais reactive with azide-tagged molecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
025010P1 Alkaline Peptone Water
Product Info
Document
Product Info
Introduction
Usages: For selective cultivation of bacteria especially Vibrio.
Principle: Peptone provide carbon and nitrogen sources; sodium chloride to maintain osmotic equilibrium; higher pH can suppress the growth of coliform and other bacteria, it is conducive to the growth of Vibrio cholerae.
How to use： 1.Suspend 18g in 1L of distilled water, stirring heated to boiling to completely dissolve, autoclave at 121℃ for 15 minutes. 2.Diluted and treated samples.
Quality control：
Item
The name and number of strain
Growth
Colony Color
1
Vibrio cholerae VbO
Good
Turbid broth
2
Escherichia coli ATCC25922
Poor
—
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Ammonia, Nitrogen, YAN
Assay Format:
Spectrophotometer, Microplate, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Decrease
Linear Range:
0.2 to 7 µg of ammonia per assay
Limit of Detection:
0.07 mg/L
Reaction Time (min):
~ 5 min
Application examples:
Grape juice, wine, fruit juices, soft drinks, dairy products (e.g. milk), dietetic food, soy sauce, eggs and egg products, cheese, meat, processed meat, seafood, bakery products (and baking agents), fertilisers, pharmaceuticals, tobacco, cosmetics, water, Kjeldahl analysis, paper (and cardboard), water and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Methods based on this principle have been accepted by MEBAK
Ammonia Assay Kit, for the rapid measurement and analysis of ammonia in all samples, including grape juice and wine (and other foods/beverages).
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
HiPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 minutes (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from FFPE tissue
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Products
RNA, miRNA
Purification method
Mini spin column
Purification technology
Silica technology, DNase
Process method
Manual (centrifugation or vacuum)
Sample type
FFPE slice, FFPE embedded tissue
Sample amount
Tissue: <6 mgFFPE: <6 slices
Yield
1-20μg
Elution volume
≥15μl
Time per run
≤30 minutes (after digestion)
Liquid carrying volume per column
800μl
Principle
This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis withproteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, RNA wasfinally eluted with low-salt buffer.
Advantages
Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Fast – several samples can be extracted in 60 minutes by column method
Safe – no phenol chloroform extraction required
Sensitive – RNA can be recovered at the level of PG
Kit Contents
Contents
IVD4144
Purification Times
50 Preps
HiPure RNA Micro Columns
50
2ml Collection Tubes
50
Proteinase K
24 mg
Protease Dissolve Buffer
1.8 ml
DNase I
600 μl
DNase Booster Buffer
1.5 ml
Buffer DPS
60 ml
Buffer FRL
15 ml
Buffer RLC
15 ml
Buffer RWC*
10 ml
Buffer RW2*
20 ml
RNase Free Water
10 ml
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature(15–25°C) does not affect their performance. The remaining kit components can be stored at roomtemperature (15–25°C) and are stable for at least 18 months under these conditions.
Experiment Data
Document
HiPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 minutes (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.
