Permagen’s  15 mL/ 50 mL Centrifuge Tube Magnetic Combo rack is designed for magnetic bead separations from up to three each, 15 mL or 50 mL Centrifuge tubes

Accommodates any common 15 mL Centrifuge Tubes and or 50 mL Centrifuge Tubes

Beads will be pulled to back wall allowing easy aspiration and tip tracking down the front wall of the tubes without disturbing bead pellet

Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads

SKU #

MSR1550

Features

• Aluminum alloy design for years of trouble-free use
• Fast magnetic bead separations
• Held to tighter tolerances for more consistent results
• Pulls beads to side of wells for easy aspiration without disturbing bead pellet 
• Rubber feet to help prevent slippage
• Stable design
• Four magnets per tube 

Compatibility

• Up to three each of 15 mL and or 50 mL Centrifuge Tubes
• Any Magnetic Beads

Volumes

Maximum Volume – 50 mL (50 mL Tube)

Minimum Volume – 7 mL (15 mL Tube)

Permagen’s 15 mL/ 50 mL Centrifuge Tube Magnetic Combo rack is designed for magnetic bead separations from up to three each, 15 mL or 50 mL Centrifuge tubes

K-MBG4

SKU: 700004319

100 assays (manual) / 400 assays (auto-analyser)

Content:	(Malt β-glucanase) 100 assays (manual) / 400 (auto-analyser) Or (Lichenase) 100 / 200 assays (manual) / 330 (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	β-Glucanase/Lichenase
Assay Format:	Spectrophotometer, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	400
Signal Response:	Increase
Limit of Detection:	(Malt β-glucanase) 4.3 x 10-4 U/mL Or (Lichenase) 9.1 x 10-5 U/mL
Reproducibility (%):	~ 3%
Total Assay Time:	(Malt β-glucanase) ~ 20 min Or (Lichenase) ~ 10 min
Application examples:	Crude malt extracts, industrial enzyme preparations.
Method recognition:	Novel method

The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(3¹-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.

Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).

Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.

Discover more assay kits for enzyme activity measurement.

Data calculators are located in the Documents tab.

Advantages

• Very cost effective 
• All reagents stable for > 2 years 
• Specific for endo-1,3:1,4-β-glucanase/lichenase 
• Simple, convenient, rapid assay 
• Well suited to automation 
• Malt flour standard and lichenase standard included

The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.

Introduction

The HiPure Mini system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Plasmid Mini Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.

Details

Specifications

Features	Specifications
Main Functions	Isolation up to 70μg plasmid DNA from 5-15ml bacterial culture
Applications	Enzyme digestion, sequencing, PCR, cloning, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Conventional plasmid, plasmid less than 30KB
Sample amount	High copy plasmid: 1-5ml culture mediumLow copy plasmid : 5-10ml culture medium
Yield	20-80µg
Elution volume	≥75μl
Time per run	Complete 1-24 samples in 30 minutes
Liquid carrying volume per column	800µl
Binding yield of column	70µg

Principle

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
• Fast – it takes only 2 minutes to obtain supernatant by optimized solution (10 minutes for other brands)
• High yield – up to 70µg plasmid can be binded in one column

Kit Contents

Contents	P100202	P100203
Purification Times	50 Preps	250 Preps
RNase A	5 mg	20 mg
Buffer P1	30 ml	140 ml
Buffer P2	30 ml	140 ml
Buffer P3	40 ml	200 ml
Buffer PW1	30 ml	140 ml
Buffer PW2*	12 ml	50 ml
Elution Buffer	15 ml	30 ml
HiPure DNA Mini Columns III	50	250 ml
2 ml Collection Tubes	50	250 ml

Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.

Purchase Guide

Name	CAT NO	Features	Sampleamount	Elutionvolume	Yield
HiPure Plasmid Mini Kit	P1001	Classic, Regular application	1-5ml LB	30-50μl	5–35µg/5ml
HiPure Plasmid Mini Kit II	P1002	Classic, Regular application	5-15ml LB	75-100μl	20-80µg/15ml
HiPure Plasmid Plus 96 Kit	P1006	Classic, Regular application	1-1.5ml LB	70-150μl	1-15μg/1ml
HiPure Fastfilter Plasmid Midi Kit	P1013	Fast	30-50ml LB	0.3-0.8ml	50–250µg/50ml
HiPure Fastfilter Plasmid Maxi Kit	P1014	Fast	100-200ml LB	0.5-1.5ml	0.4–1mg/200ml
Low endotoxin/Endotoxin-free Plasmid
HiPure Plasmid EF Maxi Kit	P1156	Classic method, General for all kinds of vectors	100-200ml LB	≥0.7ml	200–1500µg
HiPure Plasmid EF Mini Kit	P1154	Recommend for low copy vector, Thoroughly remove RNA	5-15ml LB	≥60μl	10–80µg
HiPure Plasmid EF 96 Kit	P1157	Recommend for low copy vector, Thoroughly remove RNA	1-5ml LBx96	≥75μl	30µg
HiPure Plasmid EF Midi Kit	P1231	Recommend for High copy vector, Low elution volume, High concentration	25-50ml LB	≥100μl	10-500μg
HiPure Plasmid EF Mega Kit	P1116	Recommend for High copy vector(>3μg/ml)	500ml LB	≥2ml	1-10mg

For any technical problems or customized products, please contact us.

F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here

The HiPure Mini system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Plasmid Mini Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.