
Description
Specifications:
Clone | IHC512 |
Source | Mouse Monoclonal |
Positive Control | Hairy Cell Leukemia |
Dilution Range | 1:200 |
Annexin A1 (ANXA1) is a membrane protein that plays a role in innate and adaptive immunity by controlling the biosynthesis of inflammation, prostaglandins, and leukotriene mediators. This target is overexpressed in 97% of all samples from patients with with hairy cell leukemia, and is absent in other B-cell lymphomas. High ANXA1 expression is frequently associated with advanced stage esophageal and esophagogastric junction adenocarcinoma, and is also linked to advanced and metastatic disease states.
Clone | IHC512 |
Source | Mouse Monoclonal |
Positive Control | Hairy Cell Leukemia |
Dilution Range | 1:200 |
E. coli O157:H7 is a rod-shaped, gram negative bacterium. It is an enterohemorrhagic strain of the common E. coli bacterium and infection by the O157:H7 strain is commonly associated with hemorrhagic colitis. E. coli O157:H7 is recognized by its somatic (cell wall) antigen (O157) and its flagella antigen (H7). In addition, E. coli O157:H7 is known to produce Shiga-like toxins, which cause severe symptoms. While most patients can recover from the infection, up to 15% of the patients may develop hemolytic uremic syndrome, a type of kidney failure that could be fatal. Infection of E. coli O157:H7 usually results from consumption of poorly prepared food including undercooked meat (particularly ground beef), untreated water or raw unpasteurized milk.
E.coli O157:H7 TaqMan PCR Kit, 100 reactions
E.coli O157:H7 TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
Component | Cat. TM41350 (100 preps) | Cat. TM41310 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
E. coli O157:H7 Primer & Probe Mix | 280 μL | 280 μL |
E. coli O157:H7 Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |
TGW16 Technical Parameter:
Max. Speed | 16000rpm |
Max. RCF | 19040×g |
Max. Capacity | 10×5ml |
Time Range | 0~99min59s |
RPM/RCF Convert | Yes |
Noise (dB) | ≤ 55 |
Acc/Dec | 10 Kinds |
Speed Accuracy | ±20r/min |
Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
Size (W x D x Hmm) | 355×270×205mm |
Net Weight(Kg) | 16KG |
Certificates | CE,ISO & Calibration report are available |
Matched Rotors for TGW16
Order No | Rotor | Max speed (rpm) | Max Volume(ml) | Max RCF (g) |
W16-1 | Angle rotor | 16000 | 40×0.2ml | 19040 |
W16-2 | Angle Rotor | 16000 | 24×0.5ml | 18480 |
W16-3 | Angle Rotor | 16000 | 12×1.5/2ml | 17940 |
W16-4 | Angle Rotor | 16000 | 10x5ml | 17880 |
W16-5 | Angle Rotor | 14000 | 20×1.5/2ml | 15580 |
W16-6 | Angle Rotor | 14000 | 4x8PCR | 12070 |
1. Brushless motor, no pollution, free-maintenane.
2. Microprocessor control, LCD display which indicates the speed, time, RCF in operation, 10 kinds of brake setting , operate simply.
3. Electric lid lock, super speed, imbalance protection.
4. The centrifuge body is made of high quality steel, stainless steel chamber, safe and reliable.
5. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.
6. 3 tiers protection steel cover and get the ideal centrifuation result.
Product Description
High-Sensitivity RNA Amplification Kit: Fast & Accurate Results
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature:at room temperature and constant temperature(generally 39℃~42℃),reverse transcriptaseuses specific primers and template RNA tosynthesize cDNA strands,and binds the auxiliary protein and single strand with the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology positionand strand exchange begins; accompanied by the recombinase from the complex upon dissociation,the polymerase also bindsto the 3′ end of the primer,initiating chainextension.It is suitable for laboratory-level RNA amplification and RNA amplification for other detection purposes.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
Parameters | Details |
---|---|
Product Name | RNA Isothermal Amplification Kit Basic |
Manufacturer | Amp-future |
Storage Temperature | -20°C |
Kit Components | Enzymes, Buffers ,Reagents |
Packaging | 48 Tests/box |
Detection Limit | 500-1000copies/µL |
Shipping | ICE |
Test Time | 5-20mins |
Isothermal nucleic acid Applications
Suitable for RNA isothermal rapid amplification kit(BASIC type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
RNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
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