
Description
Specifications:
Clone | IHC512 |
Source | Mouse Monoclonal |
Positive Control | Hairy Cell Leukemia |
Dilution Range | 1:200 |
Annexin A1 (ANXA1) is a membrane protein that plays a role in innate and adaptive immunity by controlling the biosynthesis of inflammation, prostaglandins, and leukotriene mediators. This target is overexpressed in 97% of all samples from patients with with hairy cell leukemia, and is absent in other B-cell lymphomas. High ANXA1 expression is frequently associated with advanced stage esophageal and esophagogastric junction adenocarcinoma, and is also linked to advanced and metastatic disease states.
Clone | IHC512 |
Source | Mouse Monoclonal |
Positive Control | Hairy Cell Leukemia |
Dilution Range | 1:200 |
DBCO-Sulfo-NHS ester is a water-soluble sulfocated reagent containing DBCO moiety. It can be used for simple and efficient labeling of antibodies, proteins and any other primary amine-bearing macromolecules with DBCO moiety in 100% aqueous buffers. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-Sulfo-NHS ester is a water-soluble sulfocated reagent containing DBCO moiety. It can be used for simple and efficient labeling of antibodies, proteins and any other primary amine-bearing macromolecules with DBCO moiety in 100% aqueous buffers. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples such as blood, serum, plasma, swab soaking solution, fluid accumulation and homogenate solution. This kit is designed to remove host cells background nucleic acid and enrich pathogen nucleic acid (including viral/bacterial/fungal DNA/RNA) from the sample. Purified DNA/RNA is ready for downstream applications such as PCR, virus detection, tNGS and other related experiments.
Specifications
Features | Specifications |
Main Functions | Extract Pathogen RNA/DNA from 0.5-1.5ml whole blood, plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution for tNGS application, remove host background nucleic acid. |
Applications | Real Time PCR, biochip analysis, NGS |
Products | Pathogen DNA / RNA |
Purification method | Polydisperse magnetic beads |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Sample type | whole blood, plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution |
Sample amount | 0.5 – 1.5 ml |
Adaptive instrument | Nucleic acid extractor, pipetting workstation |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Kit Contents
Contents | R667200C | R667202C |
Purification Times | 24 Preps | 96 Preps |
2ml Bead Tube (0.4g) | 24 | 96 |
DNase I (Powder) | 10 mg | 15 mg |
DNase Buffer | 5 ml | 20 ml |
Protease Dissolve Buffer | 3 ml | 8 ml |
Lysis Buffer LBX1 | 40 ml | 180 ml |
Buffer TL | 5 ml | 20 ml |
Proteinase K | 24 mg | 120 mg |
MagBind Particles N9 | 1.2 ml | 5 ml |
Buffer MLB | 30 ml | 120 ml |
Buffer MW1* | 13 ml | 110 ml |
Buffer MW2* | 10 ml | 50 ml |
Buffer AVE | 10 ml | 20 ml |
Storage and Stability
Proteinase K, DNase I powder and MagPure Particles N9 should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples such as blood, serum, plasma, swab soaking solution, fluid accumulation and homogenate solution. This kit is designed to remove host cells background nucleic acid and enrich pathogen nucleic acid (including viral/bacterial/fungal DNA/RNA) from the sample. Purified DNA/RNA is ready for downstream applications such as PCR, virus detection, tNGS and other related experiments.
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from bacterial culture |
Applications | PCR, southern blot and enzyme digestion, etc. |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Sample type | Gram negative or positive bacteria |
Sample amount | Bacterial culture: 0.5-1.5ml |
Elution volume | ≥60μl |
Time per run | ≤60 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Kit Contents
Contents | D513101 | D513102 | D513103 |
Purification Times | 48 Preps | 96 Preps | 480 Preps |
MagPure Particles | 1.2 ml | 2.5 ml | 11 ml |
Lysozyme | 60 mg | 120 mg | 600 mg |
Proteinase K | 24 mg | 50 mg | 220 mg |
Protease Dissolve Buffer | 3 ml | 6 ml | 30 ml |
Buffer SDS | 1.5 ml | 3 ml | 15 ml |
Buffer MLA | 30 ml | 60 ml | 300 ml |
Buffer GW1* | 13 ml | 44 ml | 110 ml |
Buffer TE | 30 ml | 40 ml | 200 ml |
Storage and Stability
MagPure Particles, Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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