
Description
Specifications:
Clone | IHC512 |
Source | Mouse Monoclonal |
Positive Control | Hairy Cell Leukemia |
Dilution Range | 1:200 |
Annexin A1 (ANXA1) is a membrane protein that plays a role in innate and adaptive immunity by controlling the biosynthesis of inflammation, prostaglandins, and leukotriene mediators. This target is overexpressed in 97% of all samples from patients with with hairy cell leukemia, and is absent in other B-cell lymphomas. High ANXA1 expression is frequently associated with advanced stage esophageal and esophagogastric junction adenocarcinoma, and is also linked to advanced and metastatic disease states.
Clone | IHC512 |
Source | Mouse Monoclonal |
Positive Control | Hairy Cell Leukemia |
Dilution Range | 1:200 |
endo-BCN-PEG6-t-butyl ester is a click chemistry linker. The hydrophilic PEG spacer increases solubility in aqueous media. The BCN group can react with azide-tagged molecules. The protected carboxyl group (COOH) prevents self coupling or polymerization under standard acid/amine or acid/hydroxyl coupling conditions. It can be converted to free acid under acidc condition. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
endo-BCN-PEG6-t-butyl ester is a click chemistry linker. The hydrophilic PEG spacer increases solubility in aqueous media. The BCN group can react with azide-tagged molecules. The protected carboxyl group (COOH) prevents self coupling or polymerization under standard acid/amine or acid/hydroxyl coupling conditions. It can be converted to free acid under acidc condition. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Usages:
For antimicrobial susceptibility testing.
Principle:
Beef extract powder and casein hydrolyzate provide nitrogen, vitamins and amino acids; soluble starch absorption of toxic metabolites.
Formulation(per liter):
Beef extract 2g
Soluble starch 1.5g
Acid hydrolysis of casein 17.5g
Final pH7.3 ± 0.2
How to use:
1.Suspend 21g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 autoclave for 15min.
2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
500g
The HiPure Plasmid EF Mini Kit combine the power of HiPure technology with Magen’s innovative endotoxin removal technology to deliver high-quality plasmid DNA with low endotoxin levels foruse in eukaryotic transfection, and in vitro experiments. The HiPure plasmid endo-free system uses a specially formulated buffer that prevents endotoxin molecules from binding to the surface of the HiPure matrix. Endotoxin contamination lowers transfection efficiencies for endotoxin sensitive celllines. For gene therapy, endotoxin contamination should be of major concernsince endotoxins have the potential to cause fever, endotoxin shock syndrome, and interfere with in vitro transfection into immune cells.
Specifications
Features | Specifications |
Main Functions | Isolation up to 80µg endotoxin-free plasmid DNA from 5-15ml bacterial culture. Recommend for low copy vector, Thoroughly remove RNA |
Applications | Cell transfection, animal injection, etc. |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Low copy plasmid vector |
Sample amount | 5-15ml LB |
Yield | 10-70μg |
Elution volume | ≥75μl |
Time per run | ≤40 minutes |
Liquid carrying volume per column | 800μl |
Binding yield of column | 70μg |
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method,then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
Kit Contents
Contents | P115402 | P115403 |
Purification Times | 50 Preps | 250 Preps |
RNase A | 5 mg | 20 mg |
Buffer P1 | 30 ml | 140 ml |
Buffer P2 | 30 ml | 140 ml |
Buffer LEN3 | 15 ml | 70 ml |
Buffer LN4 | 50 ml | 250 ml |
Buffer LN5 | 30 ml | 140 ml |
Buffer PW1 | 30 ml | 140 ml |
Buffer PW2 | 12 ml | 50 ml |
Elution Buffer | 15 ml | 30 ml |
HiPure DNA Mini Columns III | 50 | 250 |
2 ml Collection Tubes | 50 | 250 |
Storage and Stability
The kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A,Buffer P1 is stable for 6 months when stored at 2–8°C.
For any technical problems or customized products, please contact us.
F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here
The HiPure Plasmid EF Mini Kit combine the power of HiPure technology with Magen’s innovative endotoxin removal technology to deliver high-quality plasmid DNA with low endotoxin levels foruse in eukaryotic transfection, and in vitro experiments. The HiPure plasmid endo-free system uses a specially formulated buffer that prevents endotoxin molecules from binding to the surface of the HiPure matrix. Endotoxin contamination lowers transfection efficiencies for endotoxin sensitive celllines. For gene therapy, endotoxin contamination should be of major concernsince endotoxins have the potential to cause fever, endotoxin shock syndrome, and interfere with in vitro transfection into immune cells.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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