GeneAb™ KBA.62 (Melanoma Associated Antigen)

N-(Propargyl-PEG4)-N-bis(PEG4-acid) is a branched crosslinking reagent with a propargyl group and two terminal carboxylic acids. The propargyl group can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acids can react with primary amino groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

N-(Propargyl-PEG4)-N-bis(PEG4-acid) is a branched crosslinking reagent with a propargyl group and two terminal carboxylic acids. The propargyl group can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acids can react with primary amino groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.

The series of nucleic acid columns produced by Magen Biotech are based on carefully selected imported glass fiber membranes (GF/B, GF/D, GF/F). Columns production processes such as polypropylene injection molding materials, injection molding process, and downstream membrane packing and compression rings are strictly controlled. This is to ensure that the column has extremely high adsorption capacity and long-term stability. Compared with conventional products on the market, Magen’s columns are with varieties, and binding rate will not change when stored at room temperature for 4 years.

Details

Specifications

Features	Specifications
Recommended application	gDNA and RNA Isolation
Preservation conditions	Room temperature
Stability	Up to 4 years
Filter membrane	High quality glass fiber filter GF/B, 2 layers
Membrane aperture	1.0μm
Maximum binding yield of plasmid	30 μg
Maximum yield of alcohol mediated Binding	100 μg
Single liquid carrying capacity of column	900 μl
Minimum elution volume	80 μl
Withstand centrifugal force	4,000-5,000 x g
Centrifuge	Low speed centrifuge, Swing out Rotor, can placed a height of 6.5cm square,  (height of HiPure DNA Plate & 1.6ml Collection Plate: height, 6.2cm)

Adsorption Mechanism

Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silica gel membrane, while other pollutants, mainly proteins, are removed by membrane washing.

Ordering information

CAT.No.	Product Name	Package
C13131	HiPure gDNA Plate (2 x GF/B)with 1.6ml Collection Plate	10/Bag

Purchase Guide

Item No.	Product Name	Membrane type/number of layers	Collection tubes	Plasmid DNA binding capacity (Physical adsorption)	gDNA/RNA binding capacity (Alcohol-mediated adsorption)	Minimum Elution volume	Liquid volume capacity
C13010	HiPure DNA Nano Column	2 layers GF/F	2ml without cap	5μg	20μg	10μl	700μl
C13011	HiPure DNA Micro Column	3 layers GF/F	2ml without cap	10μg	50μg	15μl	700μl
C13100	HiPure DNA Mini Column I	2 layers GF/B	2ml without cap	15μg	100μg	30μl	700μl
C13110	HiPure DNA Mini Column II	4 layers GF/B	2ml without cap	35μg	200μg	50μl	800μl
C13111	HiPure RNA Mini Column	3 layers GF/B	2ml without cap	30μg	200μg	30μl	800μl
C13112	HiPure Viral Mini Column	3 layers GF/F	2ml without cap	30μg	200μg	30μl	800μl
C13113	HiPure CFDNA Mini Column	3 layers GF/F,1 layer GF/B	2ml without cap	30μg	200μg	30μl	800μl
C13120	HiPure DNA Midi Column	4 layers GF/B	15ml Centrifuge tube	125μg	1mg	500μl	4ml
C13121	HiPure DNA Midi Column III	8 layers GF/B	15ml Centrifuge tube	250μg	1mg	500μl	4ml
C13122	HiPure DNA Maxi Column	4 layers GF/B	50ml Centrifuge tube	500μg	5mg	1000μl	20ml
C13123	HiPure DNA Maxi Column III	8 layers GF/B	50ml Centrifuge tube	1mg	5mg	1000μl	20ml
C13124	HiPure DNA Maxi Column C	8 layers GF/B	50ml high speed Centrifuge tube	1mg	5mg	700μl	12ml
C13130	HiPure DNA Plate	2 layers GF/F	1.6ml Plate	30μg	100μg	80μl	900μl
C13131	HiPure gDNA Plate	2 layers GF/B	1.6ml Plate	30μg	100μg	80μl	900μl

Note: GF/B pore size is for 1.0μM glass fiber membrane; GF/F pore size is for 0.7μm glass fiber membrane.

Magen’s HiPure columns are prepared by high quality glass fiber filter membrane as raw materials through membrane cutting, membrane release, ring release, ring pressing, gland, weighing and other processes. HiPure nucleic acid adsorption columns have the characteristics of long-term stability and high binding capacity. Experiments show that the highest binding capacity and binding efficiency of HiPure nucleic acid adsorption columns are basically unchanged when stored at room temperature for 4 years.

Character

Yingtai   pilot-type   freeze   dryer   completely   simulates   the   technical   requirements   of  the   industrial   freeze   dryer,with   a   compact   and   reasonable design,a   large   redundancy   range,and   conforms   to   GMP  and   FDA   standards.The   ice   condensation   capacity   is   4~18kg,and  the  WRL  controller and   liquid   temperature   control   shelf   are   adopted,so   that   the    process   of   pre-freezing   and   freeze-drying   can    be   precisely   controlled   on   the main   machine,and   the    process   results    are   quite   comparable   with   the    production   machine.In    addition   to    supporting   the   eutectic    point   test system,pressure    rise    and     pressure    comparison     method    to    determine     the    freeze-drying    end     point    and     freeze-drying    curve    recording software,hallan   pilot-type   freeze    dryer   can   also    be   equipped   with    engineering   WRL   control   software   to   directly    Graphical   computer   control the   freeze-drying   process.It    can   be    configured   with    H202 sterilization    and   high-pressure    steam   sterilization    orintegrated   glove    box   system, which can be used for clean room operation through the wall.The cold trap and freeze-drying chamber are all designed with 316L stainless steel  to  ensure  the  cleanliness  of  the  chamber  and  good   ultimate  vacuum  and  vacuum   leakage  rate  to  the  greatest  extent.

Refrigeration Series

●  Dual  compressors,Secop  special   compressors,air  cooling (water  cooling  system  is  optional)

●   Refrigeration   method:Cascade   refrigeration    method,high   cooling efficiency,good   reliability,using   CFC-Free   environmentally   friendly refrigerant.

Silicone oil conducts heat,with uniform temperature and accuracy up to  0.1°C.Silicone oil has a wide temperature range,up to-100°C~+600°C.

● Shelf refrigeration system and cold trap refrigeration system are completely independent,without any switching,refrigeration temperature is lower and more stable.

Vacuum   series

●      Vacuum      pump:German       Pfeiffer      anti-corrosion,anti-oil      return       function vacuum   pump.(Vacuum    pump   can    be   selected    according   to    user   needs)

●       Pumping        rate:9m³/h~62m³/h,multiple        specifications       are        optional,the ultimate  vacuum  can  reach  2×10-3mbar,and  the  oil  mist  filter  is  standard.

●   The   vacuum    pipelines    are   all    high-vacuum   stainless    steel   vacuum    tubes, which  can  effectively  prevent  vacuum  leakage  and  corrosion.

●   The    double   anti-oil    return    device    completely   eliminates    the    damage   and

equipment   and   samples.

●    Automatic    backfill    air    device,the    system     has    a    built-in     High    efficiency particulate  air(HEPA)filter  to  ensure  the  complete  cleanliness  of  backfill  air.

●   The   vacuum   stabilization   system    can   stabilize   the   vacuum    degree   of   the freeze-drying    process    through     the    micro-pass    vacuum    control    valve    to ensure  that  the  samples  will   not  be  affected  by  excessive  fluctuations   in  the vacuum    degree.

●   Condensed    liquid   collection   and   discharge   system,automatically   completes the   collection   and   discharge   of   condensed   liquid   after   freeze-drying.

Control    System Characteristic

●               Control:Automatic/Manual

●   Can   store    at   least    100    independent   freeze-drying    programs,each   program    contains   32    program   segments    (Can   be

customized   according    to    user    needs),each   program   segment    corresponds    to   a    processing    stage    (pre-freezing,main

drying,post-drying).and    Include     the    following     segment    values:Cycles,shelf     temperature,vacuum    and     safety    pressure

●  All  the  equipment  can   be   recorded   in   real   time   and   the   corresponding   freeze-drying   curves   and   data  tables   can   be generated  after  the   end.The  records  of  all   data  and  curves  comply  with   the  corresponding  regulations  and  standards.

●    It    has    hierarchical    management    authority,hierarchical    management    allocation    authority    and    usage    process    tracking authority

● Remote    System

O   Fully   support   2G/4G/GSM/Wi-Fivarious   connection    modes

o  Users can remotely view all data on the device and operate it

o  Technicians  can  remotely  perform  maintenance  and  fault  detection  on  equipment,and  can  remotely  guide  and  train  users

o  Can  remotely  upgrade  and  change  the  system  of  the  equipment

Parameter

Mode	MP2	MP5	MP8	MP12
Freeze-dried area	0.24m	0.48m²	0.96m²	1.45m²
Ice condenser capacity	4kg	9kg	12kg	18kg
Shelf temperature	70℃~+80℃			65℃~+70℃
Ice condenser temperature	70 /-90℃	70/-90℃	-70℃/-90℃	-70℃/-85℃
Cold trap volume	9L	15L	18L	30L
Chamber type	single	double
Max number of shelves	2+1	5+1	8+1	12+1
Shelf size	275*400 mm	275*400 mm	365*465 mm	365*465 mm
Shelf spacing	100mm(adjustable)	100mm(adjustable)	70mm(adjustable)	70mm(adjustable)
Sample quantity (4R Cillin bottle)	558	1395	2630	4230
Shelf heating and cooling method	Thermal conducting silicone oil medium(temperature resistance:-100℃~+600℃)
Chamber and shelf surface	Atomization and electrolytic surface treatment,no dead corner residue
Pipeline diameter		中 8 9 m m
External valve port	6/	12/	18/
Equipment power	3.8kw	5.5kw	6.5kw	8kw
Equipment size	700*588*1600 mm	1030*700*1630  mm	1030*700*1850 mm	1500*850*1900 mm
Capping mode	Hydraulic system gland device/no gland			Hydraulic pressure
Suitable clean room	suitable
Vacuum sensor type	PiraniVacuum sensor/capacitive vacuum sensor
Remote control and alarm	Support 2G/4G/GSM/Wi-Fi
Terminal judgment function	Pressure contrast method         Pressure test/pressure contrast method
Freeze dryer front door cover	Stainless steel/Plexiglass			Stainless steel
Inert gas filling	(Optional)Sufficient amount and time for the program to run itself
User function customization	Can be customized and modified according to user needs
Sterilization method	HzOz/steam sterilization
Note:”/”optional.Pilot models can be customized according to user needs

Yingtai pilot-type freeze dryer completely simulates the technical requirements of the industrial freeze dryer,with a compact and reasonable design,a large redundancy range,and conforms to GMP and FDA standards.The ice condensation capacity is 4~18kg,and the WRL controller and liquid temperature control shelf are adopted,so that the process of pre-freezing and freeze-drying can be precisely controlled on the main machine,and the process results are quite comparable with the production machine.In addition to supporting the eutectic point test system,pressure rise and pressure comparison method to determine the freeze-drying end point and freeze-drying curve recording software,hallan pilot-type freeze dryer can also be equipped with engineering WRL control software to directly Graphical computer control the freeze-drying process.It can be configured with H202 sterilization and high-pressure steam sterilization orintegrated glove box system, which can be used for clean room operation through the wall.The cold trap and freeze-drying chamber are all designed with 316L stainless steel to ensure the cleanliness of the chamber and good ultimate vacuum and vacuum leakage rate to the greatest extent.