Suspend 111 g in 1 L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121°C for 15 minutes. Cool to 50°C and pour into sterile Petri dishes.
Formula (per liter)
Pancreatic digest of casein—————5g Pancreatic digest of animal tissue——5g Beef extract————————————1g D-mannitol————————————-10g Sodium chloride—————————–75g Agar———————————————15g Phenolred——————————–0.025g Final pH———————————–7.4±0.2
Storage
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Other Products
D3147 HiPure Yeast DNA Kit
Product Info
Document
Product Info
Introduction
This product provides a reliable solution for DNA isolation from yeast samples. Total DNA can be purified from yeast (<5x 107) without phenol or chloroform. The whole extraction can be finished within 60 minutes. Purified DNA can be directly used for PCR, Southern blot, ect.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from yeast cultures
Applications
PCR, southern blot and enzyme digestion, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Yeast culture
Sample amount
Bacterial culture: 1-1.5 ml
Elution volume
≥30μl
Time per run
≤60 minutes
Liquid carrying volume per column
800μl
Binding yield of column
100μg
Principle
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris,pH9.0, 0.5mm EDTA).
Advantages
Fast – several samples can be extracted in 40 minutes (after digestion)
High purity – purified DNA can be directly used in various downstream applications
Good repeatability – silica technology can obtain ideal results every time
High recovery – DNA can be recovered at the level of PG
Sufficient components – lysozyme, protease K, RNase A and glass beads
Kit Contents
Contents
D314702
D314703
Purification Times
50 Preps
250 Preps
Hipure DNA Mini Columns I
50
250
2ml Collection Tubes
50
250
Glass Beads (0.4~0.6mm)
20 g
90 g
Buffer SE
30 ml
150 ml
Lyticase
1.8 ml
5 x 1.8 ml
Buffer ATL
30 ml
150 ml
ReagentDX
500 μl
1500 μl
Buffer DL
30 ml
150 ml
Buffer GW1*
13 ml
66 ml
Buffer GW2*
20 ml
2 x 50 ml
Proteinase K
12 mg
60 mg
Protease Dissolve Buffer
1.8 ml
5 ml
Buffer AE
15 ml
30 ml
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. Buffer ATL may precipitate at low temperature. Dissolve it by 37℃ water bath.
Document
This product provides a reliable solution for DNA isolation from yeast samples. Total DNA can be purified from yeast (
Propargyl-PEG14-acid is an alkyne reagent with a carboxylic acid. The carboxylic acid can form amide bonds with amines under the activation of EDC or HATU. The alkyne group can react with azides through Click Chemistry reaction to form triazole linkage. The PEG spacer increases the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG14-acid is an alkyne reagent with a carboxylic acid. The carboxylic acid can form amide bonds with amines under the activation of EDC or HATU. The alkyne group can react with azides through Click Chemistry reaction to form triazole linkage. The PEG spacer increases the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DDL6 Refrigerated centrifuge widely used in the field of blood bank station, pharmaceutical factory and laboratory, can hold 500ml triple, 400ml triple and 250ml triple blood bags.
DDL6 Blood Bags Refrigerated Centrifuge Features:
1. Brushless frequency motor which in great torque, free maintenance, no powder pollution, quick in speed up and down.
2. Digital display which indicates the program, speed, time, RCF and temperature.
3. Micro computer control, there are 10 kinds of program and 10 kinds of acceleration/deceleration for your choice.
4. There are 6 kinds of rotors for your choice.
5. Electric lid lock, compact design, super speed and imbalance protection.
6. The centrifuge body is made of high-quality steel, safe and reliable.
