Overview

• Detection kits for ZIKV
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis

The Zika Virus (ZIKV) is an emerging mosquito-borne virus that was first identified in Uganda in 1947 in Rhesus monkeys through monitoring of sylvatic yellow fever. During large outbreaks in French Polynesia and Brazil, national health authorities reported potential neurological and auto-immune complications of ZIKV disease. Agencies investigating the Zika outbreaks are finding an increasing body of evidence about the link between ZIKV and microcephaly. Infection with ZIKV may be suspected based on symptoms and recent history (e.g. residence or travel to an area where ZIKV is known to be present). Zika virus diagnosis can only be confirmed by laboratory testing for the presence of ZIKV RNA in the blood or other body fluids, such as urine or saliva.

ZIKV TaqMan RT-PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

ZIKV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

Details

Supporting Data

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Storage Conditions

All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM62250 (100 preps)	Cat. TM62210 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
ZIKV Primer & Probe Mix	280 μL	280 μL
ZIKV Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Description 

The DM4160 FluoroBand™ XL 25 kb Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM4160 is composed of 14 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (3 kb and 1 kb) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank. 

Features

• Sharp bands
• Quick reference— enhanced bands 
• Ready-to-use— premixed with loading dye for direct loading
• Stable— room temperature storage over 6 months
• Directly observed by UV or blue light— premixed with high sensitive DNA fluorescent dye

Source 

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range 

250 ~ 25,000 bp 

Concentration 

51.6 µg/ 500 µl 

Recommended loading volume 

5 µl/ well

Storage

Protected from light 
Room temperature for 6 months
4°C for 12 months 
-20°C for 24 months 

The DM4160 FluoroBand™ XL 25 kb Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM4160 is composed of 14 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (3 kb and 1 kb) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.

Introduction

This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.

Details

Specifications

Features	Specifications
Main Functions	IVD5412 precast kit for kingfisher Flex
Applications	RT-PCR，PCR，NGS
Products	Viral DNA / RNA, body cell DNA / RNA
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technolog
Process method	Manual or automatic
Sample type
Sample amount	200μl
Adaptive instrument	Nucleic acid extractor, pipetting workstation

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.

Advantages

Kit Contents

Cat.No	Reagent	IVD5412-F-96
PK/Carrier RNA		1x 24 mg/Bottle
Protease Dissolve Buffer Blue		1.8 ml/Bottle
Tip		1
Sample Plate (DW Plate)	500µl Buffer MLB/Well	1
Wash 1 Plate (DW Plate)	500µl Buffer MW1/Well	1
Wash 2 Plate (DW Plate)	500µl Buffer CW/Well	1
Beads Plate (DW Plate)	500µl CW & 20µl MPN/Well	1
Elute Plate (KF Plate)	90µl NFW/Well	1

Storage and Stability

This kit is shipped and stored at room temperature and is valid for 12 months.

This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.