Human Immunodeficiency Virus Type 1

Overview

• Removes primer dimers
• Simultaneously clean-up and normalize PCR products 
• Fast (less than 20 minutes), high-throughput and easy processing using centrifugation
• Sufficient elution volume (100 µL) for repeat or future assays 
• Non-magnetic bead purification 

Norgen’s NGS Normalization 96-Well Kit allows for high-throughput NGS library PCR amplicon clean-up and normalization of NGS library PCR product concentration. The clean-up removes all PCR by-products including primers, dimers, enzymes and unincorporated nucleotides during the library prep PCR step. Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. The amount of Norgen’s matrix in each 96 well is optimized to have a limited binding capacity, thus each well can elute an equal concentration of PCR product. The process involves first adding 3 volumes of Buffer SK to the PCR product, and the mixture is then loaded onto the 96-Well Normalization Plate. The bound PCR amplicon is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified PCR amplicon is eluted with Elution Buffer B. The purified and normalized library PCR amplicon can then be used in NGS workflows and other sequencing applications.

Details

Supporting Data

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Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 61900 (192 preps)
Buffer SK	30 mL
Wash Solution A	2 x 38 mL
Elution Buffer B	30 mL
96-Well Normalization Plate	2
96-Well Collection Plate	2
96-Well Elution Plate	2
Adhesive Tape	1
Product Insert	1

Permagen’s  50 mL Centrifuge Tube Magnetic rack is designed for magnetic bead separations from up to six, 50 mL Centrifuge tubes

Accommodates any common 50 mL Centrifuge Tubes

Beads will be pulled to back wall allowing easy aspiration and tip tracking down the front wall of the tubes without disturbing bead pellet

Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads

SKU #

MSR6X50

Features

• Aluminum alloy design for years of trouble-free use
• Fast magnetic bead separations
• Held to tighter tolerances for more consistent results
• Pulls beads to side of wells for easy aspiration without disturbing bead pellet 
• Rubber feet to help prevent slippage
• Stable design
• Four magnets per tube 

Compatibility

• Up to six 50 mL Centrifuge Tubes
• Any Magnetic Beads

Volumes

Maximum Volume – 50 mL

Minimum Volume – 10 mL

Permagen’s 50 mL Centrifuge Tube Magnetic rack is designed for magnetic bead separations from up to six, 50 mL Centrifuge tubes

1 Layer Cell Factory Double Narrow Mouth

Huayi Cell Factory is a robust and easy-to-use cell culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.

To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.

Specifications: 1 layer 2 layers, 5 layers, 10 layers and 40 layers.

PRODUCT FEATURES

• The product is made of medical grade USP CLASS VI polymer polystyrene
• The product is made under a 100,00- class dust-free manufacturing site
• Surface TC treatment ensures efficient cell attachment
• The product assembled with ultrasonic welded technology
• Versatile port design facilitates both pouring and aseptic filling techniques
• Gamma radiation sterilization
• the cell culture surface area

of one 10-layer Cell Factory unit is equivalent to the area

of 36 T-175 flasks

Huayi Cell Factory is a robust and easy-to-use cell culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.

To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.

Specifications: 1 layer 2 layers, 5 layers, 10 layers and 40 layers.