NGS Library Quantification Kit (for Small RNA-Seq)
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Detail
Overview
Able to quantify NGS Library (Illumina) of a wide spectrum of concentrations, including sub-nanomolar concentrations
DNA is accurately quantified by using a standard curve constructed from the provided DNA Standards
Specially designed DNA standards for Small RNA-Seq library; also compatible to NGS library of other molecular weights
Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) offers a PCR-based detection procedure to quantify NGS libraries (specifically Small RNA-Seq) of a wide spectrum of concentrations. The kit consists of a specially designed primer mix compatible with the Illumina system, that is used in conjunction with the provided 2X Real-Time PCR Master Mix to amplify a library of unknown concentration. The unknown library is accurately quantified by using a standard curve constructed from the provided DNA Standard (range from 20 pM to 2 fM) on a Real-Time PCR System. The kit is specially optimized to quantify Small RNA-Seq libraries with DNA standards that have similar size to a Small RNA-Seq library. However, it could also be used with other types of NGS libraries.
Storage Conditions Upon receipt, store Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.
* Quantified Library Standards are provided as 20 pM, 2 pM, 200 fM, 20 fM and 2 fM
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TGW16 Micro High Speed Centrifuge
Product Info
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Product Info
TGW16 Technical Parameter：
Max. Speed
16000rpm
Max. RCF
19040×g
Max. Capacity
10×5ml
Time Range
0~99min59s
RPM/RCF Convert
Yes
Noise (dB)
≤ 55
Acc/Dec
10 Kinds
Speed Accuracy
±20r/min
Voltage(V/Hz)
AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)
355×270×205mm
Net Weight(Kg)
16KG
Certificates
CE,ISO & Calibration report are available
Matched Rotors for TGW16
Order No
Rotor
Max speed (rpm)
Max Volume(ml)
Max RCF (g)
W16-1
Angle rotor
16000
40×0.2ml
19040
W16-2
Angle Rotor
16000
24×0.5ml
18480
W16-3
Angle Rotor
16000
12×1.5/2ml
17940
W16-4
Angle Rotor
16000
10x5ml
17880
W16-5
Angle Rotor
14000
20×1.5/2ml
15580
W16-6
Angle Rotor
14000
4x8PCR
12070
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1. Brushless motor, no pollution, free-maintenane. 2. Microprocessor control, LCD display which indicates the speed, time, RCF in operation, 10 kinds of brake setting , operate simply. 3. Electric lid lock, super speed, imbalance protection. 4. The centrifuge body is made of high quality steel, stainless steel chamber, safe and reliable. 5. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction. 6. 3 tiers protection steel cover and get the ideal centrifuation result.
Simultaneous isolation of both host DNA and microbial DNA (universal protocol)
Fully compatible with Norgen’s Stool Nucleic Acid Collection and Transport Tubes
Eliminates PCR inhibitors including humic acids
High quality DNA for sensitive downstream applications including PCR, qPCR, Sequencing and microarray
96-Well and Magnetic Bead System formats also available
These kits provide a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. Purifies the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA can be used with a number of downstream applications.
Stool DNA Isolation Kit (Spin Column)
Universal method to detect microorganism and host cell DNA simultaneously in stool samples. Eliminates PCR inhibitors including all traces of humic acid using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.
Stool DNA Isolation 96-Well Kit (High Throughput)
Fast and easy high throughput processing using centrifugation. Eliminates PCR inhibitors including all traces of humic acid using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.
Stool DNA Isolation Kit (Magnetic Bead System)
Fast and easy processing using a magnetic bead system. Robust lysis system (chemical lysis combined with a mechanical homogenization).
Stool DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
This kit provides fast and easy processing using a magnetic bead system, and a robust lysis system (chemical lysis combined with a mechanical homogenization). High throughput and compatible with an automation robotic system.
Free Download
Extracting Biological Insights from Stool
Tips and tricks for isolating high yield and quality DNA, RNA, miRNA and EV’s from fecal samplesDownload for Free
200 mg (fresh/frozen stool) or 400 μL (preserved stool)
Type of Stool Processed
Frozen, fresh or preserved stool
Format
Spin Column
Maximum Column Binding Capacity
50 μg
Maximum Column Loading Volume
650 μL
Elution Volume
50 μL
Time to Complete 10 Purifications
30 minutes
Applications
PCR, qPCR, Southern Blot Analysis, Sequencing, Microarray Analysis.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
1000 µL filtered pipette tips for the Opentrons Flex, provided sterile in racks or blister packs (refills). Tips are clear, non-conducting polypropylene tips, free of human DNA, PCR inhibitors, RNase/DNase, pyrogens, and endotoxins. Opentrons Flex Tips and Racks are autoclavable.
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1000 µL filtered pipette tips for the Opentrons Flex, provided sterile in racks or blister packs (refills). Tips are clear, non-conducting polypropylene tips, free of human DNA, PCR inhibitors, RNase/DNase, pyrogens, and endotoxins. Opentrons Flex Tips and Racks are autoclavable.
