The Opentrons Flex HEPA/UV Module enables you to run sensitive contamination-prone applications. It removes 99.99% of 0.3 μm DNA-containing particulates and biological contaminants like bacteria, fungi, and other microorganisms from the air, creating a clean work environment inside the Opentrons Flex.
The Opentrons Flex HEPA/UV Module enables you to run sensitive contamination-prone applications. It removes 99.99% of 0.3 μm DNA-containing particulates and biological contaminants like bacteria, fungi, and other microorganisms from the air, creating a clean work environment inside the Opentrons Flex.
HiPure Plant DNA Mini Kit supplies a simple and rapid extraction of genomic DNA from different plant samples. The kit is based on silica gel column and CTAB lysis purification technology. The whole extraction process is only 30-50 minutes. Purified DNA can be used directly for PCR, SSR, AFLP, RAPD and Southern Blot, etc.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 150 mg plant and fungal tissue |
| Applications | PCR, SSR, AFLP, RAPD and southern blot, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Fresh / frozen plant samples, dried plant / seed samples |
| Sample amount | Fresh / frozen plant samples: 50-150 mgDried plant / seed samples: 15-40 mg |
| Elution volume | ≥40μl |
| Time per run | 30-50 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
This product is based on silica column purification. The sample is lysed with CATB Buffer. DNA is released into the lysate. Cell debris, precipitated proteins and polysaccharides are removed by chloroform extraction. After adjust the binding condition, transfer to an adsorption column. DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Kit Contents
| Contents | D318702 | D318703 |
| Purification Times | 50 Preps | 250 Preps |
| Buffer PAL | 60 ml | 200 ml |
| Buffer GWP | 60 ml | 200 ml |
| Buffer DW1 | 30 ml | 150 ml |
| Buffer GW2* | 20 ml | 50 ml |
| Buffer AE | 20 ml | 60 ml |
| HiPure DNA Mini Columns II | 50 | 250 |
| 2 ml Collection Tubes | 50 | 250 |
Storage and Stability
This product can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
| Name | CAT NO | Fresh / frozen tissue amount | Dried tissue amount | Column type | Elution volume | Yield (muscle) |
| HiPure HP Plant DNA Kit | D3187 | 150mg | 40mg | 1.5ml column | 50 – 100μl | 3-70μg |
| HiPure HP Plant DNA Maxi Kit | D3163 | 5g | 1g | 50ml column | 0.7 – 3ml | 75-1250μg |
| HiPure SF Plant DNA Kit | D3164 | 100mg | 20mg | 1.5ml column | 50 – 100μl | 3-50μg |
| HiPure SF Plant DNA 96 Kit | D3167 | 50mg | 15mg | 96 well plate | 75 – 150μl | 3-30μg |
HiPure Plant DNA Mini Kit supplies a simple and rapid extraction of genomic DNA from different plant samples. The kit is based on silica gel column and CTAB lysis purification technology. The whole extraction process is only 30-50 minutes. Purified DNA can be used directly for PCR, SSR, AFLP, RAPD and Southern Blot, etc.
TCO-PEG6-DBCO is a PEG6 linker featuring a trans-cyclooctene and a DBCO group. DBCO is a click chemistry handle which easily reacts with azides, while the TCO function readily reacts with tetrazine groups in target compounds.
TCO-PEG6-DBCO is a PEG6 linker featuring a trans-cyclooctene and a DBCO group. DBCO is a click chemistry handle which easily reacts with azides, while the TCO function readily reacts with tetrazine groups in target compounds.
Description
The ExcelDye™ 6× DNA Loading Dye (Tri-Color) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains three dyes (Xylene cyanol FF, Bromophenol blue, Orange G) for tracking the DNA migration. The Xylene cyanol FF, Bromophenol blue and Orange G migrate at approximately 800 bp, 150 bp and 30 bp on a standard 2% TAE agarose gel respectively (4,000 bp, 500 bp and 50 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Composition
Storage
4°C for 12 months
-20°C for 36 months
The ExcelDye™ 6× DNA Loading Dye (Tri-Color) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains three dyes (Xylene cyanol FF, Bromophenol blue, Orange G) for tracking the DNA migration. The Xylene cyanol FF, Bromophenol blue and Orange G migrate at approximately 800 bp, 150 bp and 30 bp on a standard 2% TAE agarose gel respectively (4,000 bp, 500 bp and 50 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
