Propargyl-PEG13-t-butyl ester

Attogene’s Organophosphate detection kit is in designed specifically to detect Organophosphate in liquid samples.

Description

Organophosphate compounds (OP) account for the largest class of rural and urban poisons in the world that are used to kill pests but can also be toxic to humans.  OPs cause toxicity by means of blocking the acetylcholinesterase enzyme (AchE).  The AChE-directed OPs react with a serine residue that is located at the catalytic site found within the AChE gorge. The OP targeted enzyme is no longer able to hydrolyze ACh, resulting in the buildup of ACh in the nerve synapse. This effect causes excessive excitation of the nerves, producing uncoordinated movements, tremors, paralysis and death.  Both synthetic and natural(Guanitoxin) organophosphates are dangerous to humans — exposure can lead to visual, coordination, muscular, and neurological deficiencies, and in some cases even to death.  In turn, exposure to OP is a significant public health concern which would significantly benefit from an improved detection platform.

Attogene’s Organophosphate detection kit is in designed specifically to detect Organophosphate in liquid samples.  For solid samples a simple sample preparation method is performed.  The ability to detect Organophosphate is performed is simple and sensitive.  The reaction uses a chromophore that can be detected by eye. In the presence of Organophosphate, the rate of chromophore production is reduced in a concentration dependent fashion.  The higher the concentration of Organophosphate the less color is produced.

Attogene’s Organophosphate detection kit is in designed specifically to detect Organophosphate in liquid samples.

The ChIP-Seq Library Prep Kit (illumina and MGI Platform) was developed for the construction of high quality ChIP-Seq libraries using 5 ng to 400 ng of ChIP DNA as input. The kit is compatible with ChIP DNA fragments generated from both enzymatic methods and physical methods (sonication, nebulization etc.).

ChIP-Seq Library Prep Kit Workflow

ChIP-Seq is the combination of chromatin immunoprecipitation (ChIP) with next generation sequencing. It is a powerful tool for the analysis of global transcription factors and other proteins in diseases and biological pathways, and characterization of histone modifications in a genome-wide level at single-base resolution. ChIP-Seq delivers whole genome level of functional profiling of global transcription factors, and provides better understanding of epigenetic modifications.

Three index types are available for the ChIP-Seq Library Prep Kit of the illumina platform:

Non-index (Cat.# 30032): Libraries do not have index.

Index (Cat.# 30034): Each index primer contains a unique 6-base index sequence can be used for identification. 48 samples can be pooled together. Index information can be downloaded here.

Unique dual index (Cat.# 30036): The ChIP-Seq library multiplexing for 96 samples is possible. Our unique 4-Base Difference Index System have 8 bases index length and at least 4 bases are different from each other for better library identification. Our unique dual indexing primers remove sequencing errors such as index hopping, index contamination, mis-assignment, and other errors. Index information can be downloaded here.

Indexes are available for the MGI platform kits (Cat.# 34034).

Kit advantages:

• Super fast protocol
  • Library prep can be done in 1.5 hours
  • The hands-on time is only around 10 minutes
• Easy procedure
  • Ready-to-use master mix simplified the procedure
  • Less reaction components make it easy to setup reactions
• Reduced more than half of the beads cost
• Input ChIP DNA: From 5 ng to 400 ng

Comparison of library conversion efficiency under the same condition. Input DNA amounts are 5 ng and 30 ng. BioDynami ChIP-Seq Library Prep Kit (Cat.# 30034) was used.

Comparison of aligned reads, aligned rate and duplication rate. Input DNA amounts are 5 ng and 30 ng. BioDynami ChIP-Seq Library Prep Kit (Cat.# 30034) was used.

Data comparison: Input DNA amounts are 5 ng and 30 ng. BioDynami ChIP-Seq Library Prep Kit (Cat.# 30034) was used. Sequencing peak regions are shown.

The ChIP-Seq Library Prep Kit (illumina and MGI Platform) was developed for the construction of high quality ChIP-Seq libraries using 5 ng to 400 ng of ChIP DNA as input. The kit is compatible with ChIP DNA fragments generated from both enzymatic methods and physical methods (sonication, nebulization etc.).

Introduction

029161 Catalase Detection Reagent

Usage:For Catalase Detection.

Specification:2ml*10vials

2ml*10vials