Introduction
This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA spin column to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.
Details
Specifications
| Features | Specifications |
| Main Functions | Co-isolation DNA and RNA(not include miRNA) from a single sample (cells, soft tissue, plant sample) |
| Applications | RT-PCR, cDNA synthesis, PCR andsecond-generation sequencing, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Soft tissue samples (viscera, excluding skin andmuscle), cultured cells and common plant tissues |
| Sample amount | Soft Tissue: < 30mg, Cell: <1 x 107, Plant: <100mg |
| Yield | DNA: 1 – 20 μg, RNA: 3 – 100 μg |
Principle
The Kits are designed to purify both genomic DNA and total RNA from the same cellor tissue sample. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column and bind DNA. Ethanol is added to the flow-through and the sample is applied to an RNA column. DNA/RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit. High-quality DNA is eluted in as little as 50µl water using the Kit.
Advantages
- High quality – high purity total RNA / DNA can be directly used in a variety of downstream applications
- Fast – column method can complete the extraction of several samples in 30 minutes
- Safe – no phenol chloroform extraction required
- Simultaneous extraction- simultaneously isolate DNA and RNA from one sample
Kit Contents
| Contents | R511102 | R511103 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure DNA Mini Columns | 50 | 250 |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Collection Tubes | 100 | 2 x 250 |
| Buffer RLC | 50 ml | 200 ml |
| Buffer DW1 | 30 ml | 150 ml |
| Buffer RW1 | 30 ml | 150 ml |
| Buffer RW2* | 20 ml | 2 x 50 ml |
| RNase Free Water | 10 ml | 30 ml |
| Buffer AE | 10 ml | 50 ml |
Storage and Stability
HiPure DNA/RNA Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
