R6611 MagPure Blood RNA Kit

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This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples.

Detail

Introduction

This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation total RNA from 1-1.5ml whole blood, 0.5-1ml bone marrow, buffy coat
ApplicationsRT-PCR, cDNA synthesis, second generation sequencing
Purification methodPolydisperse magnetic beads
Purification technologyMagnetic beads technology
Process methodManual or automatic
Sample typeanticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells
Sample amount1-1.5ml whole blood, 0.5-1ml bone marrow
Yield1-30μg


Principle

This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples. This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested by lysis buffer and protease, and RNA/DNA is released into the lysis buffer. Add binding solution and magnetic particles to adsorb RNA/DNA, while proteins are not adsorbed and removed. The particles adsorbed with DNA/RNA are washed with washing buffer to remove proteins and other impurities, then washed with ethanol to remove salt, and finally digested with DNase to remove DNA. RNA is recovered by adding binding solution, and finally the RNA is eluted with low salt buffer. The eluted RNA can be directly used for experiments such as RT-PCR,NGS and virus detection.

Advantages

  • High purity – OD 260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
  • Economy – less than 50% of the price of top brands
  • Automatic – extraction without manual participation, saving time and effort
  • High yield – nucleic acid recovery up to 95%
  • General – samples include whole blood, serum, plasma, lymphocyte suspension, bone marrow, cultured cells, etc.

Kit Contents

ContentsR661101R661102R661103
Purification Times48 Preps96 Preps480 preps
10 x RBC Lysis Buffer50 ml2 x 50 ml4 x 100 ml
Proteinase K12 mg24 mg120 mg
Protease Dissolve Buffer1.8 ml1.8 ml10 ml
DNase I600 μl2 x 600 μl10 x 600 µl
DNase Buffer20 ml30 ml150 ml
MagPure Particles N1.2 ml2.5 ml11 ml
Buffer RTL30 ml60 ml300 ml
Buffer ALB240 ml60 ml300 ml
Buffer MW1*22 ml44 ml220 ml
Buffer MW2*20 ml50 ml2 x 100 ml
RNase Free Water10 ml20 ml60 ml

Storage and Stability

DNase I should be shipped with ice pack or dry ice and stored at -20°C upon arrival. MagPure Particles N and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage(up to 8 weeks) at room temperature (15–25°C) does not affect their performance.The remaining kit components can be store at room temperature and are stable for up to 18 months under these conditions.

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