The Raffinose/D-Galactose test kit allows for the specific and rapid measurement of raffinose and D-galactose in plant materials and food products.
Detail
K-RAFGA
SKU: 700004331
120 assays per kit
Content:
120 assays per kit
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
D-Galactose, Raffinose
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
4 to 83 µg of D-galactose per assay (i.e. approx. 12 to 250 µg of raffinose per assay)
Limit of Detection:
21 mg/L
Reaction Time (min):
~ 60 min
Application examples:
Cereal flours, soybean flour, by-products of sucrose manufacture and other materials.
Method recognition:
Used and accepted in food analysis
The Raffinose/D-Galactose test kit allows for the specific and rapid measurement of raffinose and D-galactose in plant materials and food products.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Very rapid reaction due to inclusion of galactose mutarotase (patented technology)
Very competitive price (cost per test)
All reagents stable for > 2 years after preparation
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Other Products
t-Boc-N-Amido-PEG4-propargyl
Product Info
Document
Product Info
t-Boc-N-Amido-PEG4-propargyl is a propargyl linker with one side protected by Boc group. The propargyl group can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The Boc protected amine can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
t-Boc-N-Amido-PEG4-propargyl is a propargyl linker with one side protected by Boc group. The propargyl group can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The Boc protected amine can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from blood, buffy coat, tissue and other samples
Applications
Second generation sequencing, PCR, real time PCR, etc.
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
High purity – OD 260 / 280 : 1.7- 1.9, OD 260 / 230 : 1.5 – 2.0
Economy – less than 50% of the price of Qiagen and other imported products
High yield – most optimal process, ensuring the recovery up to 90%
Strong processing ability – samples including animal blood, cultured cells, animal tissues, etc.
Kit Contents
Contents
IVD3102
Purification Times
200
MagPure Particles
5 ml
Proteinase K
100 mg
Protease Dissolve Buffer
10 ml
Rnase A
40 mg
Buffer ATL
60 ml
Buffer AL
60 ml
Buffer BD*
20 ml
Buffer BW1*
110 ml
Elution Buffer
30 ml
Storage and Stability
Proteinase K, RNase A, MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
NGS Cell Free DNA Library Prep Kit (illumina and MGI Platforms)
Product Info
Document
Product Info
The NGS Cell Free DNA Library Prep Kit (illumina and MGI Platforms) was developed for the construction of high quality cell-free DNA (cfDNA) libraries using 1 ng to 50 ng of cell-free DNA as input. The kit has a simple work flow and a fast procedure. Multiplexing of the cell free DNA library is possible based on the index type.
NGS Cell Free DNA Library Prep Kit Workflow
The main source of cell-free DNA (cfDNA) is derived from apoptotic hematopoietic cells in blood and found in the plasma. The length of the cfDNA is about 150-200 bp in length. Circulating tumor DNA (ctDNA) derived from malignant tumors is a part of cfDNA. Both cfDNA and ctDNA can be used as a noninvasive biomarker since it offers a better approach in comparison to invasive tissue biopsies.
NGS has been used for cfDNA and ctDNA sequencing in the field of liquid biopsy as it provides a whole genome level of molecular profiling. One of the hurdles for cfDNA sequencing is the difficulty of library preparation from the limited amount of cfDNA obtained from plasma. Our cell-free NGS kit makes it easy to get enough libraries from limited input in just 1.5 hours.
Three index types are available for the NGS Cell Free DNA Library Prep Kit of the illumina platform:
Non-index (Cat.# 30029): Libraries do not have index.
Index(Cat.# 30031): Each of our index primers contains a unique 6-base index sequence that can be used for sample identification. Total 48 library multiplexing is possible. Index information can be downloaded here.
Unique dual index (Cat.# 30033): Cell-free DNA library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a Four-Base Difference Index System. The system have at least 4 bases different from each other in the 8 bases index length. The primers effectively minimize sequencing errors such as mis-assignment, index hopping, index contamination etc. Index information can be downloaded here.
Indexes are available for the MGI platform kits (Cat.# 34031).
Kit advantages:
Fast and simple protocol
Hands-on time is around 10 minutes
The total protocol time is only 1.5 hours
Easy procedure based on:
Ready-to-use master mix for easy setup of reactions
Less reaction components to simplify bench work
Less magnetic beads used for cleanup procedures: This can reduce more than 50% of the beads consumption
Guaranteed high library conversion efficiency
Low input cell-free DNA: From 1 ng to 50 ng
Comparison of library conversion efficiency with different samples under the same condition.
Comparison of library yield with different samples under the same condition.
Document
The NGS Cell Free DNA Library Prep Kit (illumina and MGI Platforms) was developed for the construction of high quality cell-free DNA (cfDNA) libraries using 1 ng to 50 ng of cell-free DNA as input. The kit has a simple work flow and a fast procedure. Multiplexing of the cell free DNA library is possible based on the index type.
