The Raffinose/Sucrose/D-Glucose test kit is for the measurement and analysis of D-glucose, sucrose and raffinose, stachyose and verbascose in seeds and seed meals. Based on the measurement of D-glucose on enzymic hydrolysis of raffinose, stachyose and verbascose to D-glucose, D-fructose and D-galactose.
Detail
K-RAFGL
SKU: 700004332
120 assays of each per kit
Content:
120 assays of each per kit
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
D-Glucose, Raffinose, Sucrose
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
510
Signal Response:
Increase
Limit of Detection:
100 mg/L
Reaction Time (min):
~ 20 min
Application examples:
Analysis of grain legumes and other materials containing raffinose, stachyose and verbascose.
Method recognition:
Used and accepted in food analysis
The Raffinose/Sucrose/D-Glucose test kit is for the measurement and analysis of D-glucose, sucrose and raffinose, stachyose and verbascose in seeds and seed meals. Based on the measurement of D-glucose on enzymic hydrolysis of raffinose, stachyose and verbascose to D-glucose, D-fructose and D-galactose.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection of numerous significant pathogens. The genesigPLEX range provides the most efficient method of detecting DNA and RNA by combining multiple assays into a single tube. This principle of multiplexing represents state of the art PCR testing and is a key feature of the high-throughput testing conducted by laboratories around the world. These assays include process controls to verify the quality of the nucleic acid extraction and eliminate false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the handbook.
Packaged, optimised and ready to use. Expect Better Data.
Document
Precise reproducible results Time saving through completing multiple tests in one go Cost saving through a reduced price compared with buying four kits separately Exceptional value for money Supplied lyophilised – no cold chain shipping
Well-accepted microRNA sequence used for normalization in gene expression studies
Best suited for RNA purification from samples with low RNA abundance including liquid biopsies (plasma/serum/urine etc.) and low cell or tissue inputs
Compatible to expression analysis using RT-qPCR – both RNA and matching forward PCR primer provided.
Fully compatible to Norgen’s microScript cDNA Synthesis system
Fully compatible to Next Generation Sequencing (Small RNA-Seq) library preparation workflow
The amount of RNA that can be extracted from different biological or clinical samples varies greatly. For example, while a few micrograms of RNA could be easily purified from tissues and cells in excess amounts (such as from a few milligrams of tissue), many liquid biopsy samples may yield very low amounts of RNA. In fact, samples such as urine or plasma may yield 1 – 100 ng or less RNA per 100 µL of sample. Such a range of RNA quantity is often below the detection limit of most commonly used techniques for measuring RNA including nano-spectrophotometry and fluorescent nucleic acid stains. As a result, without properly determined RNA concentration, it becomes very difficult to normalize the starting quantity of RNA used in gene expression studies.
Norgen’s microRNA (cel-miR-39) Spike-In Kit offers a quantified synthetic RNA (cel-miR-39) for spike-in during RNA extraction procedures and subsequent normalization in RT-qPCR assays. The amount of cel-miR-39 RNA recovered after RNA extraction is directly correlated with the amount of total RNA recovered. After reverse transcription (such as with Norgen’s microScript Reverse Transcription system) of the sample RNA (with spike-in), the level of cel-miR-39 can be determined by subjecting the cDNA generated to quantitative PCR (qPCR) using fluorescent nucleic acid stains such as SYBR Green. A cel-miR-39 specific primer is included in the kit. The level of expression of any target transcripts in different RNA samples can now be normalized to the cel-miR-39 transcript level using standard method such as ∆∆Ct relative quantification.
In addition, the cel-miR-39 RNA is compatible to library preparation methods (including ligation-based protocols) in Next Generation Sequencing (Small RNA-Seq) workflows. The cel-miR-39 RNA could be used for normalization as well as for tracking library construction efficiency.
Storage Conditions Upon receipt, store Norgen’s microRNA (cel-miR-39) Spike-In Kit at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.
