Screening of saxitoxin in shellfish samples as low as 50ppb in sample Format: 25 tests (12 tests, 12 controls) Filters, syringes, extract collection tubes, buffers Run Time: 30 Minutes
Detail
Screening of saxitoxin in shellfish samples as low as 50ppb in sample
Saxitoxins (STXs) are naturally occurring alkaloids produced by some marine dinoflagellates and by strains of various species of freshwater cyanobacteria. Saxitoxin is one of the prevalent paralytic shellfish toxins (PSTs). It belongs to a family of potent neurotoxins with a molecular weight around 300 Da. Saxitoxin and its derivatives are alkaloids composed of a tetrahydropurine ring system with a highly polar guanidinium group. Due to their significant toxicity, saxitoxins are closely monitored in marine environments where they can accumulate in the food chain during harmful algal blooms (HABs). An action level of 800ppb, or 80ug per 100grams of shellfish, has been established.
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IST-102 ClearASeal WeldTM Heat Sealing Film
Product Info
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Product Info
Overview
Optically clear heat sealing film which is non-peelable and non-pierceable. The seal is suitable for qPCR, optical applications and storage.
Heat sealing offers a 100% effective method for plate sealing for a complete seal integrity, as well as being quick and cost effective
Our ClearASeal Weld is an optically clear polymer film forming a permanent seal to polypropylene plates
The optical clarity of this seal enables its use for sealing plates required for imaging use, including fluorescent detection methods such as qPCR and colorimetric assays
The ClearASeal Weld forms a complete seal to a plate, enabling both low and very high temperature uses, including low temperature storage and high temperature incubations
This seal is suitable for PCR/qPCR, even without the use of a pressurized heated lid, and is 100% effective when used in water bath thermal cyclers
The permanent nature of this 100% effective seal renders it suitable for the storage and disposal of hazardous material
ClearASeal Weld demonstrates a good solvent resistance and can be utilized for long term compound storage
This seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
Also available in multiple roll formats compatible with specified automated heat sealers, such as our Wasp or Chameleon XT
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Optically clear heat sealing film which is non-peelable and non-pierceable. The seal is suitable for qPCR, optical applications and storage.
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REQUIRED COMPONENTS
qPCR machine or Thermocycler + Fluorescent plate reader
PCR plate or equivalent and appropriate optically clear seal
This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from 1-1.5ml whole blood, 0.5-1ml bone marrow, buffy coat
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells
Sample amount
1-1.5ml whole blood, 0.5-1ml bone marrow
Yield
1-30μg
Principle
This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples. This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested by lysis buffer and protease, and RNA/DNA is released into the lysis buffer. Add binding solution and magnetic particles to adsorb RNA/DNA, while proteins are not adsorbed and removed. The particles adsorbed with DNA/RNA are washed with washing buffer to remove proteins and other impurities, then washed with ethanol to remove salt, and finally digested with DNase to remove DNA. RNA is recovered by adding binding solution, and finally the RNA is eluted with low salt buffer. The eluted RNA can be directly used for experiments such as RT-PCR,NGS and virus detection.
Advantages
High purity – OD 260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
Economy – less than 50% of the price of top brands
Automatic – extraction without manual participation, saving time and effort
High yield – nucleic acid recovery up to 95%
General – samples include whole blood, serum, plasma, lymphocyte suspension, bone marrow, cultured cells, etc.
Kit Contents
Contents
R661101
R661102
R661103
Purification Times
48 Preps
96 Preps
480 preps
10 x RBC Lysis Buffer
50 ml
2 x 50 ml
4 x 100 ml
Proteinase K
12 mg
24 mg
120 mg
Protease Dissolve Buffer
1.8 ml
1.8 ml
10 ml
DNase I
600 μl
2 x 600 μl
10 x 600 µl
DNase Buffer
20 ml
30 ml
150 ml
MagPure Particles N
1.2 ml
2.5 ml
11 ml
Buffer RTL
30 ml
60 ml
300 ml
Buffer ALB2
40 ml
60 ml
300 ml
Buffer MW1*
22 ml
44 ml
220 ml
Buffer MW2*
20 ml
50 ml
2 x 100 ml
RNase Free Water
10 ml
20 ml
60 ml
Storage and Stability
DNase I should be shipped with ice pack or dry ice and stored at -20°C upon arrival. MagPure Particles N and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage(up to 8 weeks) at room temperature (15–25°C) does not affect their performance.The remaining kit components can be store at room temperature and are stable for up to 18 months under these conditions.
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This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples.
