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SD-25/SD-25C Vacuum Centrifugal Concentrator

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Key features:
1. High-definition LCD touch screen, simultaneously displaying operational parameters: speed, temperature, vacuum, and running time.
2. Delayed start: after reaching a fixed speed, Start the vacuum pump to effectively prevent sample mixing.
3. Fully automatic vacuum control system, vacuum setting range0.1~1000mbar, Control accuracy0.1mbar.
4. 1.5/2ml Rotor stacking for simultaneous use; rotor replacement without tools.
5. Programmable with temperature and pressure settings; capable of storing 36 programs
6. Compatible with various rotor heads; transparent glass window for easy observation.
7. Magnetic drive: maintenance-free, non-contact drive, fully sealed.
8. Safety in use: inductive electric self-suction door lock ensures the centrifugal concentrator can only start when the door cover is fully closed and locked.
9. Can be used in conjunction with freeze-drying systems
Model:
SD-25/SD-25C

Brand:
Yingtai

Detail

SD-25/SD-25C Vacuum Centrifugal Concentrator

Ambient temperature type/Refrigerated type

Technical Parameter

Temperature rangeRoom Temperature~+80℃-10℃~+80℃
Temperature Accuracy士1℃
Max.Speed2500rpm
Max.Capacity6x100ml
Timer0~99h59min
Vacuum control range0.1~1000mbar
Main unit withstands vacuum levels0.01mbar
Noise≤55dBA≤65dBA
Voltage0.7kw1.3kw
Power supply220v,50Hz
Dimension(L×W×Hmm)360*448*304mm440*653*420mm

Adapter Rotor

Rotor Type            Rotor VolumeRotor Type    Rotor Volume
Angle Rotor42×1.5/2mlAngle Rotor8×50ml
Angle Rotor108×1.5/2mlAngle Rotor6×100ml
Angle Rotor48×5mlAngle Rotor16*20ml Sample vial
Angle Rotor30×10mlAngle Rotor16*5ml+8*4ml+8*3ml
Angle Rotor24×15mlMicroplate Rotor2×96well

Other Products

D3126 HiPure FFPE DNA Kit

Introduction

HiPure FFPE Nucleic acid Kit supplies a simple and rapid DNA extraction for formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 20 minutes (not including digestion time). DNA can be directly used for downstream applications such as PCR, southern blot and viral DNA detection, etc.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation total DNA from FFPE tissue samples
ApplicationsPCR, southern blot and viral DNA detection, etc.
Purification methodMini spin column
Purification technologySilica technology
Process methodManual (centrifugation or vacuum)
Sample typeFormalin-fixed, paraffin-embedded (FFPE) tissue and sections samples
Sample amount<20mg
Elution volume>15µl
Time per run≤20 minutes
Liquid carrying volume per column4ml
Binding yield of column100μg

Principle

Hipure FFPE Nuclear acid kit adopts silica gel column purification. The sample is deparaffinated by xylene and digested by lysate and protease. After de crosslinked at 90 ℃, DNA/RNA is released into the lysate. Adding ethanol to adjust the binding conditions, the sample is transferred to the column where DNA/RNA is adsorbed on the membrane and protein is removed without adsorption. Protein and other impurities are washed by buffer GW1, and the salt is removed by buffer GW2. Finally, the DNA / RNA is eluted by low salt buffer.

Advantages

  • Safety – deparaffinating without contacting with xylene or other toxic solution
  • Fast – without overnight incubation and digestion, several samples can be extracted within 2hours
  • High efficiency -remove the formaldehyde modification of DNA, greatly enhancing the sensitivity of PCR

Kit Contents

ContentsD312602D312603
Purification Times50 Preps250 Preps
HiPure DNA Mini Columns I50250
2ml Collection Tubes50250
Buffer DPS30 ml150 ml
Buffer ATL15 ml60 ml
Buffer AL15 ml60 ml
Buffer GW1*22 ml88 ml
Buffer GW2*12 ml50 ml
Proteinase K24 mg120 mg
Protease Dissolve Buffer1.8 ml10 ml
Buffer AE10 ml30 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data

Aluminum Block – Flat Bottom (Flex)

The Opentrons Flat Bottom Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block can hold flat bottom plates and other flat labware at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2). This version is only compatible for the Opentrons Flex.

D3139 HiPure Insect DNA 96 Kit

Introduction

HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation total DNA from insect tissue
ApplicationsPCR, southern bolt and virus detection, etc
Purification method96 well DNA plate
Purification technologySilica technology
Process methodManual (centrifugation or vacuum)
Sample typeInsect tissue samples
Sample amount
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column

Principles

This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

  • High purity DNA – can be used in sensitive downstream applications such as multiplex and quantitative pcr
  • Good repeatability – suitable for extracting high-yield DNA from insect tissue samples
  • Safe – without phenol chloroform extraction and alcohol precipitation

Kit Contents

ContentsD313901D313902
Purification Times1 x 964 x 96
HiPure DNA Plate14
2.2 ml Collection Plate14
1.6 ml Collection Plate14
0.5ml Collection Plate14
Seal Film832
Buffer ITL30 ml120 ml
Buffer IL30 ml125 ml
Buffer GW144 ml2 x 110 ml
Buffer GW250 ml3 x 50 ml
Proteinase K50 mg200 mg
Protease Dissolve Buffer6 ml15 ml
Buffer AE20 ml60 ml

Storage and Stability

Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.