t-Boc-N-amido-PEG4-Amide-Tri-(propargyl-PEG10-ethoxymethyl)-methane is a branched crosslinker molecule with three terminal propargyl groups and a t-Boc protected amide group. The propargyl groups can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The protected amine can be deprotected under acidic conditions. Reagent grade, for research use only.
Detail
t-Boc-N-amido-PEG4-Amide-Tri-(propargyl-PEG10-ethoxymethyl)-methane is a branched crosslinker molecule with three terminal propargyl groups and a t-Boc protected amide group. The propargyl groups can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The protected amine can be deprotected under acidic conditions. Reagent grade, for research use only.
Other Products
TD5A Tabletop Low Speed Centrifuge
Product Info
Document
Product Info
TD5A Low Speed Centrifuge
Features:
1. Microprocessor control, digital display, touch panel, parameters in running can be edited and change to display other running parameter and RCF.
2. Brushless frequency motor with simpler construction, more reliable performance, longer life and quietly running.
3. Automatic lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.
4. Adapters are available by experiment requirements.
5. 3 tiers protection steel cover, safe and reliable.
6. TUV CE EMC certificate.
TD5A Technical Parameter:
Max. Speed
5000rpm
Max. RCF
4730×g
Max. Capacity
4×300ml
Time Range
0~99min
RPM/RCF Convert
Yes
Noise (dB)
≤ 58
Temperature
Normal
Acc/Dec
10 Kinds
Speed Accuracy
±20r/min
Temperature Accuracy
/
Voltage(V/Hz)
AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)
620×450×355mm
Net Weight(Kg)
52KG
Certificates
CE,ISO & Calibration report are available
Matched Rotor for TD5A
Order NO.
Rotor Type
Max speed(r/min)
Volume (ml)
Max RCF(*g)
5A-1
Swing rotor (round cup)
4000
4×300ml
3180
5A-2
Swing rotor (square cup)
4000
4×300ml
3150
5A-3
Swing rotor
4000
4×24×7ml
3150
4000
4×24×5ml
2750
4000
4×18×10ml
3140
5A-4
Microplate rotor
4000
2×4×96 well
2910
5A-5
Microplate rotor
4000
2×4×48 well
2300
5A-6
Swing rotor
5000
4×1×50ml
4730
5000
4×1×100ml
4730
5A-7
Swing rotor
4000
4×2×50ml
3200
4000
4×2×100ml
3020
4000
4×4×15ml
3200
4000
4×6×15ml
3200
4000
4×8×15ml
3200
4000
4×12×5ml vacuum tube
2480
4000
4×12×7ml vacuum tube
2760
4000
4×12×10ml vacuum tube
2790
4000
4×8×10/7/5ml vacuum tube
2790
4000
4×6×10/7/5ml vacuum tube
2790
4000
4×4×10/7/5ml vacuum tube
2790
5A-8
Fixed Rotor
5000
6×15ml
2540
5A-9
Fixed Rotor
5000
12×15ml
3080
5A-10
Fixed Rotor
5000
24×15ml
3500
5A-11
Fixed Rotor
5000
30×15ml
3830
5A-12
Fixed Rotor
5000
4×50ml
2520
5A-13
Fixed Rotor
5000
6×50ml
2850
5A-14
Fixed Rotor
5000
12×50ml
3860
5A-15
Fixed Rotor
4000
24×50ml
2970
5A-16
Fixed Rotor
5000
4×100ml
2630
5A-17
Fixed Rotor
5000
6×100ml
3130
5A-18
Fixed Rotor
4000
12×100ml
2970
Document
TD5A centrifuge Max Capacity 4x300ml swing out rotor, max speed 5000rpm, can hold 96 vacuum tubes
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Formaldehyde
Assay Format:
Spectrophotometer, Microplate, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
0.1 to 14 μg of formaldehyde per assay
Limit of Detection:
0.033 mg of formaldehyde per test or 0.016 mg/mL of formaldehyde in a sample treated as per the standard procedure
Limit of Quantification:
0.11 mg of formaldehyde per test or 0.054 mg/mL of formaldehyde in a sample treated as per the standard procedure
Reproducibility (%):
~ 3%
Reaction Time (min):
~ 15 min
Application examples:
Environmental samples, food and beverage samples.
The Formaldehyde Assay Kit provides a simple robust method for the measurement of formaldehyde.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
HiPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 minutes (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from FFPE tissue
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Products
RNA, miRNA
Purification method
Mini spin column
Purification technology
Silica technology, DNase
Process method
Manual (centrifugation or vacuum)
Sample type
FFPE slice, FFPE embedded tissue
Sample amount
Tissue: <6 mgFFPE: <6 slices
Yield
1-20μg
Elution volume
≥15μl
Time per run
≤30 minutes (after digestion)
Liquid carrying volume per column
800μl
Principle
This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis withproteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, RNA wasfinally eluted with low-salt buffer.
Advantages
Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Fast – several samples can be extracted in 60 minutes by column method
Safe – no phenol chloroform extraction required
Sensitive – RNA can be recovered at the level of PG
Kit Contents
Contents
IVD4144
Purification Times
50 Preps
HiPure RNA Micro Columns
50
2ml Collection Tubes
50
Proteinase K
24 mg
Protease Dissolve Buffer
1.8 ml
DNase I
600 μl
DNase Booster Buffer
1.5 ml
Buffer DPS
60 ml
Buffer FRL
15 ml
Buffer RLC
15 ml
Buffer RWC*
10 ml
Buffer RW2*
20 ml
RNase Free Water
10 ml
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature(15–25°C) does not affect their performance. The remaining kit components can be stored at roomtemperature (15–25°C) and are stable for at least 18 months under these conditions.
Experiment Data
Document
HiPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 minutes (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.