t-Boc-N-amido-PEG4-Amide-Tri-(propargyl-PEG10-ethoxymethyl)-methane

Overview

• Ensure optimal results for sensitive applications like NGS
• Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
• Concentration of small amounts of RNA into 8 μL
• Ideal for concentrating RNA purified from exosomes, plasma, serum, urine, and other bodily fluids and any RNA samples initially purified in large volumes
• Efficient RNA cleanup from enzymatic reactions – labeling, DNase treatment and in vitro transcription
• Cleanup of RNA isolated using different methods, including phenol/chloroform extractions
• Fast and easy processing using rapid spin-column format
• Suitable for all sizes of RNA, from large rRNA down to microRNA (miRNA)
• No phenol or chloroform extractions
• Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary separation matrix.

Norgen’s EXTRAClean RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup and concentration of up to 10 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. The minimum recommended elution volume is 8 μL, which enables the concentration of small amounts of all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other reaction components such as proteins, RNases and nucleotides, without the use of phenol or chloroform. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays and next generation sequencing.

Details