Tetra(3-methoxy-N-(PEG2-prop-2-ynyl)propanamide) Methane

Overview

• No need to immediately process samples
• Total DNA, including viral DNA, preserved at room temperature over 4 months
• Total RNA, including viral RNA, preserved at room temperature over 2 months
• Inactivate microorganisms including bacteria, fungi, yeast and viruses
• Inactivates SARS-CoV-2 virus – Explore the Application Note
• Ship swab samples at room temperature safely
• Compatible with most DNA and RNA isolation methods
• CE-IVD marked version available

Norgen’s Total Nucleic Acid Preservation Tubes are designed for ambient preservation and transport of total nucleic acids (DNA and RNA) from samples collected using a swab, including nasal, buccal, saliva, fecal, skin, surfaces, etc. The Total Nucleic Acid Preservation Tubes contain Norgen’s Total Nucleic Acid Preservative in a liquid format. The user simply collects the specimen using a sterile nylon flocked swab (not provided), and then transfers the swab into the Total Nucleic Acid Preservative. The preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Total Nucleic Acid Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Preservative allow DNA samples to be stored at room temperature for over 4 months and RNA samples to be stored at room temperature for over 2 months.
Swab samples collected and preserved using Norgen’s Total Nucleic Acid Preservation Tubes are compatible with most commercially available DNA and RNA isolation methods. Samples stored in the tubes have been used successfully with most of Norgen Biotek’s DNA and RNA isolation kits and reagents, including Norgen’s Total RNA Purification Kit (Cat# 17200) and Norgen’s Saliva DNA Isolation Kit (Cat# RU45400). For purification of total nucleic acids please refer to the supplementary protocol provided with Norgen’s Total RNA Purification Kit (Cat#17200).

Details

Supporting Data

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Kit Specifications
Stability of DNA at Room Temperature	4 months
Stability of RNA at Room Temperature	2 months

Storage Conditions and Product Stability

The Total Nucleic Acid Preservation Tubes can be stored at room temperature for up 1 year without any reduction in kit performance. The collect before date is indicated on the tube label and the kit box label.

Kit Components	Cat. 69200
Total Nucleic Acid Preservation Tubes	50
Product Insert	1

OverView

T7 RNA Polymerase catalyzes the formation of RNA from a DNA template in the 5’-3’ direction and is commonly used in in vitro transcription (IVT) applications. The enzyme is T7 promoter specific, requires Mg²⁺ as a cofactor and can use modified nucleotides for the synthesis. The T7 RNA Polymerase requires a double-stranded DNA template and can produce full-length RNA transcripts.

Key Features

• T7 promoter-specific RNA polymerase
• Available in standard glycerol-based formulation as well as lyophilization-friendly formulation without glycerol.

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Suggested Applications

• In vitro transcription of RNA
• Molecular diagnostics (NASBA and other)

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Properties

Quality Control

ArcticZymes is dedicated to the quality of our products. T7 RNA Polymerase is manufactured at our ISO 13485 certified facility in Norway.‍

T7 RNA Polymerase catalyzes the formation of RNA from a DNA template in the 5’-3’ direction and is commonly used in in vitro transcription (IVT) applications. The enzyme is T7 promoter specific, requires Mg2+ as a cofactor and can use modified nucleotides for the synthesis. The T7 RNA Polymerase requires a double-stranded DNA template and can produce full-length RNA transcripts.