Tetra(3-methoxy-N-(PEG2-prop-2-ynyl)propanamide) Methane

Introduction

Intended Use

For the selective isolation and culture of Listeria monocytogenes.

Principle and Interpretation

Peptone provides the carbon and nitrogen sources necessary for growth; yeast extract powder and starch provide carbon and nitrogen sources, vitamins and growth factors; sodium chloride can maintain a balanced osmotic pressure; glucose provides a carbon source; Listeria hydrolyzes esculin and reacts with iron ions to form black 6,7-dihydroxycoumarin; mannitol is a fermentable sugar, and phenol red is a pH indicator; lithium chloride and other antibiotics can inhibit Gram-negative bacteria and most Gram-positive bacteria; agar is the coagulant of the culture medium.

Formulation

Ingredients	/liter
Peptone	23 g
Starch	1 g
NaCl	5 g
Columbia agar	13 g
Mannitol	10 g
Ferric ammonium citrate	0.5 g
Esculin (aesculin)	0.8 g
Dextrose (glucose)	0.5 g
Lithium chloride	15.0 g
Phenol red	0.08 g
pH7.2±0.2 at 25°C

Preparation

Weigh 68.9g of dry powder of this product, add 1L of distilled water or deionized water, stir, heat and boil until completely dissolved, divide into Erlenmeyer bottles, sterilize at 121℃ for 15min, cool to room temperature and set aside. Heat to dissolve agar before use, cool to 50℃, add 1 tube of supporting reagent (SR0140) per 100mL of basal culture medium, shake well and pour into sterilized culture dish.

Quality Control

The following quality control strains were inoculated and cultured at 35-37℃ for 24h. The results are as follows:

Quality control strains	Growth
Listeria monocytogenes ATCC19115	Gray-green colonies with a black depression in the center and black surrounding
Enterococcus faecalis ATCC29212	–
Escherichia coli ATCC25922	–

Storage and Shelf Life

2-30℃，Keep container tightly closed, avoid direct sunlight.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Intended Use For the selective isolation and culture of Listeria monocytogenes. Principle and Interpretation Peptone provides the carbon and nitrogen sources necessary for growth; yeast ex……

Overview

• Detection kits for HCV
• Available in TaqMan format for analysis

Hepatitis C virus infection is a major cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma (HCC) worldwide. Neither a vaccine nor an effective treatment is available for HCV. Pegylated interferon-α (PEG-IFN- α), combined with ribavirin, is the current standard therapy. Its efficiency ranges between 20-80%, depending on the HCV genotype. Unfortunately, many HCV infected patients do not respond to or do not tolerate the IFN-based therapy. Therefore, the number of patients progressing to HCC, as a result of HCV infection, is expected to increase over the next 20-30 years. The natural history of the disease reveals the elusive nature of the virus in a number of features. First, the infection is often detected incidentally at the time of blood donation as the acute infection is clinically asymptomatic in most patients. Second, HCV successfully escapes multi-specific host immune responses in the majority of patients which establishes persistent infection. Third, a significant number of persistently infected individuals remain unaware of the infection for decades, until liver fibrosis, cirrhosis and/or hepatocellular carcinoma develop.

HCV TaqMan RT-PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

HCV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

Figure 1 / 3

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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Component	Cat. TM37650 (100 preps)	Cat. TM37610 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
HCV Primer & Probe Mix	280 μL	280 μL
HCV Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1