For use with the Total Dietary Fiber Assay Kit. Contains barley β-glucan, high amylose maize starch, wheat starch, casein, pectin and larch galactan. Wheat arabinoxylan is available on request.
Detail
K-TDFC
SKU: 700004345
Contains 6 Controls: Use with K-TDFR, K-INTDF or K-RINTDF
Content:
Contains 6 Controls: Use with K-TDFR, K-INTDF or K-RINTDF
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: Ambient, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Dietary Fiber
For use with the Total Dietary Fiber Assay Kit. Contains barley β-glucan, high amylose maize starch, wheat starch, casein, pectin and larch galactan. Wheat arabinoxylan is available on request.
Our SNPsig® kits use our own proprietary genotyping method to enable the identification of SARS-CoV-2 variants of concern. These products can be used on any real-time PCR machine using familiar protocols, whilst resulting in exceptional genotyping data.
Positive control templates for wild-type and variants are supplied in every kit to make data interpretation simple.
Our SNPsig® technology provides an alternative to sequencing as well as S gene target failure (SGTF) that enables scientists to analyse and monitor these specific genomic mutations. Our kits can provide a pivotal role in screening for SARS-CoV-2 variants for the purpose of genomic surveillance and studies.
Document
For the detection of the SARS-CoV-2 variants with the VUI-21APR-01 and VUI-21APR-03 (India)
Rapid detection of specific detection profiles
High priming efficiency
Sensitive to < 100 copies of target
Positive copy number standard curve for quantification
Accurate controls to confirm findings
96 reactions, includes master mix
This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 3g of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic, mitochondrial, and chloroplast) for reliable PCR and southern blot in less than 1 hour.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 3g plant and fungal tissue
Applications
PCR, SSR, AFLP, RAPD and southern blot, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Various plant samples (including conventional, polysaccharides and polyphenols)
This product is based on silica Column purification. Plant material is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged.DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
Advantages
Broad spectrum – suitable for extracting DNA from various plant samples
Fast – several samples can be extracted in 30 minutes by silica technology
High purity – high quality DNA, completely remove inhibitors
High yield – silica technology can achieve the highest yield
Kit Contents
Contents
D316302
D316303
Purification Times
10 Preps
50 Preps
RNase A
20 mg
90 mg
Protease Dissolve Buffer
1.8 ml
10 ml
Buffer PAL
180 ml
900 ml
Buffer GWP
150 ml
800 ml
Buffer GW2
25 ml
200 ml
Buffer AE
30 ml
120 ml
HiPure DNA Maxi Columns II
10
50
50 ml Collection Tubes
20
100
Storage and Stability
RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 3g of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic, mitochondrial, and chloroplast) for reliable PCR and southern blot in less than 1 hour.
Room Temperature DNA Amplification Kit For Isothermal Nucleic Acid
Product Info
Document
Product Info
Product Description
Room Temperature DNA Amplification Kit for Isothermal Nucleic Acid
Product Detail
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
Parameters
Details
Product Name
DNA Isothermal Amplification Kit NFO
Manufacturer
Amp-future
Storage Temperature
-20°C
Kit Components
Enzymes, Buffers ,Reagents
Packaging
48 Tests/box
Detection Limit
500-1000copies/µL
Shipping
ICE
Test Time
5-20mins
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.