DNA amplification in a flexible liquid format. Contains all the enzymes and reagents necessary for the amplification of DNA, the user need only supply primers, dNTPs and template. See manual for more information. Click to order oligonucleotides.
DNA amplification in a flexible liquid format. Contains all the enzymes and reagents necessary for the amplification of DNA, the user need only supply primers, dNTPs and template. See manual for more information. Click to order oligonucleotides.
Perfect for:
• End-point gel electrophoresis DNA detection
• down-stream applications (e.g. sub-cloning)
• facilitating use of different RPA reaction volumes, or variation of component ratios.
Product code: TALQBAS01
For simplified collection and shipment of leaf samples for our premium DNA Extraction and Genotyping Lab Services.
About
3CR Bio’s Leaf Sample Collection Kit – Your Gateway to High-Quality DNA Extraction and Genotyping Lab Services
The correct sampling and storage of leaf samples for DNA extraction have a significant impact on the final quality and quantity of the DNA extracted. Our simple, lightweight kits provide everything you need to sample and ship your leaf tissue from anywhere in the world, so it reaches us in top condition.
Use the 3CR Bioscience Leaf Sample Collection Kit to sample and prepare leaf tissue optimally when shipping to us for DNA extraction and Genotyping. Full instructions available in the Leaf Sample Collection User Guide.
Kit Contents
or
The Plant Kit is used in combination with our Lab Services.
Visit the Lab Services page for more information about our DNA Extraction, Endpoint Genotyping, and GBTS services.
Here you can also:
Seamless, reliable, and very competitive on price. We look forward to working with you.
For simplified collection and shipment of leaf samples for our premium DNA Extraction and Genotyping Lab Services.
The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA and miRNA from 0.2-0.6ml serum and plasma |
| Applications | |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | |
| Sample amount | |
| Yield | |
| Elution volume | |
| Time per run | |
| Liquid carrying volume per column | |
| Binding yield of column |
HiPure RNA technology simplifies total RNA isolation. The sample material is denatured in Lysis Buffer. The protein is then precipitated by Protein Precipitation Solution and pelleted by centrifugation. After the removal of protein the binding conditions for nucleic acids are adjusted by adding isopropanol. Total nucleic acids are bound to the column. Optionally, DNA can be removed by an on-column DNase digest. The remaining nucleic acids are washed and eluted with minimal amounts of RNase-Free water.
Kit Contents
| Contents | R431402 | R431403 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure RNA Mini Columns | 100 | 2 x 250 |
| 2ml Collection Tubes | 100 | 2 x 250 |
| Buffer CFL | 6 ml | 30 ml |
| Buffer CFP | 1.8 ml | 10 ml |
| Buffer RWC | 20 ml | 80 ml |
| Buffer RW2* | 20 ml | 2 x 50 ml |
| RNase Free Water | 10 ml | 60 ml |
Storage and Stability
The kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Usages:
Yeast extract is made from yeast, used in microbiological culture media as a source of nitrogen and B vitamins.
Technical specification:
Total nitrogen(TN)—————————≥10.0%
Amino nitrogen(AN) ————————≥4.0%
Moisture—————————————≤5.0%
Ash———————————————≤10.0%
Clˉ——————————————–＜2.0%
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.
Specifications: 500g/bottle; 10kg/bag
500g/bottle; 10kg/bag
