DNA amplification in a flexible liquid format. Contains all the enzymes and reagents necessary for the amplification of DNA, the user need only supply primers, dNTPs and template. See manual for more information. Click to order oligonucleotides.
DNA amplification in a flexible liquid format. Contains all the enzymes and reagents necessary for the amplification of DNA, the user need only supply primers, dNTPs and template. See manual for more information. Click to order oligonucleotides.
Perfect for:
• End-point gel electrophoresis DNA detection
• down-stream applications (e.g. sub-cloning)
• facilitating use of different RPA reaction volumes, or variation of component ratios.
Product code: TALQBAS01
Features:
1.Microprocessor control, digital display, touch panel, parameters in running can be edited and change to display other running parameter and RCF.
2.Brushless frequency motor with simpler construction, more reliable performance, longer life and quietly running.
3.Automatic lid lock, LCD display, It can store 100 programs. super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.
4.Adapters are available by experiment requirements.
5.3 tiers protection steel cover, safe and reliable.
6.IEC CE EMC certificate.
TD5 Technical Parameter：
| Max. Speed | 5000rpm |
| Max. RCF | 4730×g |
| Max. Capacity | 4×250ml |
| Time Range | 0~99min |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 58 |
| Temperature | Normal |
| Acc/Dec | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Temperature Accuracy | / |
| Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
| Size (W x D x Hmm) | 570×400×320mm |
| Net /Gross weight(Kg) | 42KG /47KG |
| Certificates | CE,ISO & Calibration report are available |
Matched Rotors for TD5
| Order No | Rotor | Max | Volume(ml) | Max RCF(×g) |
| Speed(r/min) | ||||
| D5-1 | Swing rotor(round | 4000 | 4×250ml | 2880 |
| cup) | ||||
| D5-2 | Swing buckets | 4000 | 4×16×7ml | 2860 |
| D5-3 | 4000 | 4×16×5ml | 2470 | |
| D5-4 | 4000 | 4×13×10ml | 2830 | |
| D5-5 | 5000 | 4×1×50ml | 4730 | |
| Swing rotor | ||||
| D5-6 | 4×1×100ml | 4730 | ||
| D5-7 | 4×2×100ml | 3020 | ||
| D5-8 | 4×2×50ml | 3020 | ||
| D5-9 | 4×4×15ml | 3020 | ||
| D5-10 | 4×6×15ml | 3020 | ||
| D5-11 | 4×4×10/7 | 2770 | ||
| D5-12 | 4×4×5 vacuum tube | 2360 | ||
| ####### | adapters | |||
| D5-13 | 4×6×10/7 | 2770 | ||
| D5-14 | 4×6×5 vacuum tube | 2360 | ||
| adapters | ||||
| D5-15 | 4×8×10/7 | 2770 | ||
| D5-16 | 4×8×5 vacuum tube | 2360 | ||
| adapters | ||||
| D5-17 | 4×12×5 vacuum tube | 2480 | ||
| D5-18 | 4×12×7 vacuum tube | 2760 | ||
| D5-19 | 4×10×10 vacuum tube | 2880 | ||
| D5-20 | Microplate rotor | 4000 | 2×3×96 well | 2310 |
| D5-21 | Fixed rotor | 5000 | 6×15ml | 2540 |
| D5-22 | 5000 | 12×15ml | 3080 | |
| D5-23 | 5000 | 24×15ml | 3500 | |
| D5-24 | 5000 | 30×15ml | 3830 | |
| D5-25 | 5000 | 4×50ml | 2520 | |
| D5-26 | Fixed rotor | 5000 | 6×50ml | 2850 |
| D5-27 | 4000 | 12×50ml | 2470 | |
| D5-28 | 5000 | 4×100ml | 2630 | |
| D5-29 | 5000 | 6×100ml | 3130 |
Adapter for 250 round cup and square cup
| VolumeCup | 5ml | 7ml | 10ml | 15ml | 50ml |
| 250ml（Square） | 9pcs/cup | 9 | 9 | 4 | 1 |
| 250ml（Round） | 7pcs/cup | 7 | 7 | 4 | 1 |
Rotor with adapter
The NGS Low Input DNA Library Prep Kit (illumina and MGI Platforms) was developed for construction of high quality NGS libraries with low input DNA amount from 1 ng to 400 ng. The kit allows scientist to study samples with limited DNA such as tumor samples, patient samples, and other specially collected samples (FACS sorting etc.). The kit has high library conversion efficiency with as little as 1 ng DNA input. The fast and simple 1.5-hour protocol makes libraries with even coverage and low GC-bias based our unique chemistry for DNA end-polishing and ligation.
The kit needs double strand DNA fragments (blunt and/or sticky) as input DNA for NGS library preparation, and is compatible with DNA fragments generated from both enzymatic approach (for example, BioDynami DNA fragmentation enzymes, Cat.# 40061 and Cat.# 40062) and mechanical approaches such as sonication and nebulization etc.
NGS Low Input DNA Library Prep Kit Workflow
Three index types are available for the NGS Low Input DNA Library Prep Kit of illumina platform:
Non-index (Cat.# 30022): Libraries do not have index.
Index (Cat.# 30024): Each of our index primers contains a unique barcode sequence with 6 bases that can be used to identify the low input DNA libraries. Library multiplexing up to 48 samples is possible. Index information can be downloaded here.
Unique dual index (Cat.# 30025): Library multiplexing up to 96 low input DNA libraries is possible with unique dual indexes with our Four-Base Difference Index System. The system allows us to make indexes for the libraries that have at least 4 bases different from each other in the 8-base barcode length. Our unique dual index primers can reduce sequencing errors such as de-multiplexing errors, amplification errors, mis-assignment of reads, index cross-contamination, and also index hopping. The kit includes 96 pre-mixed unique pairs of index primers. Index information can be downloaded here.
Indexes are available for the MGI platform kits (Cat.# 34024).
Kit advantages:
Comparison of library conversion efficiency under the same NGS library preparation condition. Input DNA amounts are 1 ng and 10 ng, respectively. DNA was mechanical sheared with Covaris before library prep. BioDynami kit: NGS Low Input DNA Library Prep Kit (Cat. #30022).
Comparison of library yield under the same NGS library prep condition. Input DNA amounts are 1 ng, 10 ng, and 100 ng. DNA was mechanical sheared with Covaris before library prep. BioDynami kit (Cat. #30022). PCR cycle numbers were indicated.
The NGS Low Input DNA Library Prep Kit (illumina and MGI Platforms) was developed for construction of high quality NGS libraries with low input DNA amount from 1 ng to 400 ng. The kit allows scientist to study samples with limited DNA such as tumor samples, patient samples, and other specially collected samples (FACS sorting etc.). The kit has high library conversion efficiency with as little as 1 ng DNA input. The fast and simple 1.5-hour protocol makes libraries with even coverage and low GC-bias based our unique chemistry for DNA end-polishing and ligation.
Tri(propargyl-NHCO-ethyloxyethyl)amine is a crosslinker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Tri(propargyl-NHCO-ethyloxyethyl)amine is a crosslinker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
