DNA amplification in a flexible liquid format. Contains all the enzymes and reagents necessary for the amplification of DNA, the user need only supply primers, dNTPs and template. See manual for more information. Click to order oligonucleotides.
DNA amplification in a flexible liquid format. Contains all the enzymes and reagents necessary for the amplification of DNA, the user need only supply primers, dNTPs and template. See manual for more information. Click to order oligonucleotides.
Perfect for:
• End-point gel electrophoresis DNA detection
• down-stream applications (e.g. sub-cloning)
• facilitating use of different RPA reaction volumes, or variation of component ratios.
Product code: TALQBAS01
Sample type purification kit guide
The 16S V1-V3 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V3 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V1-V3 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V3 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
Figure 1 / 3
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| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V1-V3 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Step | Component | Cat. 70200 (24 preps) | Cat. 70210, 70220, 70230, 70240 (96 preps) |
|---|---|---|---|
| Amplicon PCR (PCR 1) | MGX Master Mix | 330 µL | 1,320 µL |
| 16S V1-V3 Primer Mix | 70 µL | 280 µL | |
| Index PCR (PCR 2) | Indexing Master Mix | 660 µL | 2 x 1,320 µL |
| N7xx Index Primer | 50 µL | 50 µL | |
| S5xx Index Primer | 70 µL | 70 µL | |
| PCR Clean-Up | Resuspension Buffer | 2 x 1,250 µL | 2 x 5,000 µL |
| Nuclease-free water | 1,250 µL | 1 x 6,000 µL |
TGL18 Tabletop 21000rpm High Speed large capacity Swing out rotor 4x800ml universal Refrigerated Centrifuge Adapters for 5/10/15/50ml tubes. And holding Angle Rotors like 24×1.5/2ml, 6x50ml, 12x15ml…in high speed separation smooth.
TGL18 Universal Refrigerated Centrifuge Application:
Holding lots of Swing Out Rotors & Angle Rotors for options. Mainly used in animal and plant molecular biology, cell biology, food safety, life science, agriculture and forest science, animal husbandry science, biological products, pharmaceutical products and other fields of ideal equipment for the separation and precipitation of samples preparation.
TGL18 Universal Refrigerated Centrifuge Features:
1. With silent-block and shock absorbers that guarantees smooth and quiet operations & fast cooling.
2. The centrifuge body is made of high-quality steel, safe and reliable.
3. Microprocessor digital control of all functions:speed, time,
temperature, acceleration/deceleration, RCF, 10 program memory, operation fault display.
4. Electronic safety lock alarm inside, just press down the top lid until it will lock in sealed and open up after separation automatically.
5. Easy open the centrifuge lid via pneumatic springs; the operation area of the centrifuge is at the front end.
6. RPM/RCF adjustable along with the speed setting calculating automatically.
7. 10 selective acceleration and deceleration rates for options
8. The self-diagnostic system provides a protection for imbalance over speed/voltage and electronic lock fault.
TGL18 Universal Refrigerated Centrifuge Technical Parameters:
| Max Speed | 21,000r/min | Max RCF | 30,910xg |
| Max Volume | 4x800ml | Power supply | AC220V,50HZ,7A |
| Timer | 1~9h/59min | Noise | ≤58dBA |
| Dimension | 700×715×420mm | Net Weight | 125KG |
| Speed Accuracy | ±20r/min | Package(W*D*H) | Plywood box: 880x800x580mm |
TGL18 Universal Refrigerated Centrifuge Matched Rotors:
| Order No. | Rotor type | Max.Speed (rpm) | Max.Volume (ml) | Max.RCF (xg) |
| No30671 | Swing out rotor | 4,000 | 4x800ml | 3,450 |
| Adapter for 4x23x5ml | 3,450 | |||
| Adapter for4x19x10nl | 3,450 | |||
| Adapter for4x14x15ml | 3,450 | |||
| Adapter for4x5x50ml | 3,450 | |||
| No30801 | Angle rotor | 21,000 | 12×1.5/2ml | 30,910 |
| No30802 | Angle rotor | 15,000 | 40×0.5ml | 22,920 |
| No30803 | Angle rotor | 17,000 | 24×1.5/2ml | 26,460 |
| No30804 | Angle rotor | 13,500 | 30×1.5/2ml | 19,340 |
| No30844 | Angle rotor | 13,000 | 48×1.5/2ml | 17,930 |
| No30805 | Angle rotor | 16,000 | 16x5ml | 22,020 |
| No30806 | Angle rotor | 16,000 | 12x7ml | 21,380 |
| No30807 | Angle rotor | 10,000 | 12x15ml | 11,840 |
| No30808 | Angle rotor | 15,000 | 12x10ml | 22,680 |
| No30809 | Angle rotor | 15,000 | 8x20ml | 22,680 |
| No30811 | Angle rotor | 13,000 | 6x50ml | 18,840 |
| No30814 | Angle rotor | 12,000 | 6x100ml | 16,390 |
The Corners of our Company____________________________________
Metal housing with 3-tiers protection. It can hold both swing out rotors and angle rotors in low speed and high speed spining to switch smoothly. And its Quiet noise, Less vibration & Fast cooling guarantee a perfect separation.
Propargyl-PEG3-alcohol consists of a hydroxyl group and a propargyl group. The propargyl group reacts with azide compounds in copper catalyzed azide-alkyne Click Chemistry to form a triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG3-alcohol consists of a hydroxyl group and a propargyl group. The propargyl group reacts with azide compounds in copper catalyzed azide-alkyne Click Chemistry to form a triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
