The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits are designed for the rapid removal of endotoxins from previously purified proteins or peptides, with protein recoveries of > 95% being achieved. Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses. Due to the high toxicity of endotoxins in vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications. These kits efficiently reduce endotoxin levels to ≤ 0.01 EU/mg of protein, using spin column chromatography based on Norgen’s proprietary resin as the separation matrix. These kits are highly efficient in removing many different salts commonly used in the laboratory including, but not limited to, MgCl2, NaCl, KCl, CaCl2, LiCl and CsCl. The purified protein samples can be used in a number of downstream applications including sequencing, cloning, and in vitro and in vivo introduction into cells and organisms for various purposes. The simultaneous removal of salts while concentrating a dilute protein solution makes the kit a convenient method for preparing proteins before running many downstream applications such as SDS-PAGE, isoelectric focusing, X-ray crystallography, NMR spectroscopy, mass spectroscopy and other applications.
Mini Kit
The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit is designed for the rapid removal of endotoxins from up to 200 μg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.
Maxi Kit
The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit is designed for the rapid removal of endotoxins from up to 4 mg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.
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| Kit Specifications | |
| Maximum Protein Input | 4 mg |
| Minimum Protein Input | 0.25 mg |
| Maximum Column Volume Input | 18 mL |
| Molecular Weight of Recovered Proteins | No Molecular Weight cut-off |
| Final Endotoxin Levels | ≤ 0.01 EU/μg protein |
| Protein Recovery | 90-95% |
| % Detergent Removal | 90-95% |
| Detergents that can be Removed | Including Triton® X-100, CHAPS, NP-40 and Tween 20 |
| Elution Volume | 2-4 mL |
| Time to Complete 10 Purifications | 30-40 minutes |
Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 22800 (25 preps) | Cat. 22200 (4 preps) |
|---|---|---|
| Binding Buffer J | 8 mL | 8 mL |
| Binding Buffer N | 4 mL | 20 mL |
| Wash Solution M | 50 mL | 130 mL |
| Wash Solution CIP | 20 mL | 60 mL |
| Wash Solution N | 30 mL | 130 mL |
| Wash Solution NIP | 20 mL | 60 mL |
| Elution Buffer G | 6 mL | 20 mL |
| Endotoxin Removal Solution | 1.5 mL | 1.5 mL |
| Protein Neutralizer EF | 2 mL | 2 mL |
| Maxi Spin Columns (assembled with Collection Tubes) | – | 4 |
| Mini Spin Columns | 25 | – |
| Collection Tubes | 25 | – |
| Maxi Spin Columns (assembled with Collection Tubes) | 4 | |
| Elution Tubes (1.7 mL) | 25 | – |
| Elution Tubes (50 mL) | 4 | – |
| Product Insert | 1 | 1 |
Propargyl-PEG4-methylamine is a PEG linker that can be react with azide compounds in copper catalyzed azide-alkyne Click Chemistry reactions. The methylamine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The PEG spacer increases hydrophilicity of the linker in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG4-methylamine is a PEG linker that can be react with azide compounds in copper catalyzed azide-alkyne Click Chemistry reactions. The methylamine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The PEG spacer increases hydrophilicity of the linker in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The RNA Clean Beads use paramagnetic bead technology for high-throughput purification of RNA or cDNA from in vitro applications such as transcription, antisense RNA (aRNA) amplification and RNA and cDNA probe synthesis. The resulting purified product can be used in the following applications: PCR and RT-PCR, probes for microarray or macroarray, RNase protection assays, transfection for RNAi experiments and cDNA synthesis and labeling.
Specifications
| Features | Specifications |
| Main Functions | Recovery of RNA from RNA reaction solution by magnetic bead method (Replace Beckmen RNAClean XP) |
| Applications | Advanced applications, such as sequencing, genechip, fluorescence quantification, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | RNA products, restriction endonuclease systems, or other enzymatic reaction solutions |
| Sample amount | 20-100µl |
| Recovery | 90% |
| Elution volume | ≥20μl |
| Operation time | ≤50 minutes |
RNA Clean utilizes an optimized buffer to selectively bind RNA or cDNA to paramagnetic beads. Excess oligonucleotides, nucleotides, salts, and enzymes can be removed using a simple washing procedure.
Advantages
Kit Contents
| Contents | BRP-5 | BRP-50 | BRP-500 |
| RNA Clean Beads | 5 ml | 50 ml | 500 ml |
Storage and Stability
Store at 4℃ upon arrival, for up to 12 months. For best results shake the reagent well until all of the beads are completely in suspension and aliquot RNAClean Beads into RNase free containers. Do not pour remaining reagent back into the storage container. Mix RNAClean Beads well before use. The reagent should appear homogenous and consistent in color.
DO NOT FREEZE.
The RNA Clean Beads use paramagnetic bead technology for high-throughput purification of RNA or cDNA from in vitro applications such as transcription, antisense RNA (aRNA) amplification and RNA and cDNA probe synthesis. The resulting purified product can be used in the following applications: PCR and RT-PCR, probes for microarray or macroarray, RNase protection assays, transfection for RNAi experiments and cDNA synthesis and labeling.