The 50 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains ten discrete fragments ranging from 50 bp to 500 bp with a higher intensity reference band at 250 bp. This Ladder is ideal for quick sizing of PCR products
Contents 1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Ladder Properties: • Ten discrete bands, ranging from 50 bp to 500 bp • Higher intensity band at 250 bp for easy reference
Fragment
Size (bp)
Mass (ng)
1
500
79
2
450
67
3
400
59
4
350
55
5
300
49
6
250
76
7
200
33
8
150
22
9
100
31
10
50
29
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Other Products
Urine Exosome Purification Kit
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Product Info
Overview
Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media and saliva in less than 30 minutes.
Versatile sample input ranging from 250 µL to 30 mL
Urine Exosome Purification Mini Kit (250 µL – 1 mL urine)
Urine Exosome Purification Midi Kit (2 mL – 10 mL urine)
Urine Exosome Purification Maxi Kit (11 mL – 30 mL urine)
Exosome purification is based on Norgen’s proprietary resin separating matrix through exosomes’ surface proteins.
No precipitation reagents, overnight incubation, protease or coagulant treatments required
No time-consuming ultracentrifugation, filtration or special syringes required
Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
Purified exosomes are compatible with functional studies.
Purified exosomes are free from any protein-bound cell-free circulating RNA
Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration.
Exosomal RNA can be extracted from the purified exosomes using Norgen’s Exosomal RNA Purification technology or any other RNA extraction method.
The Urine Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different urine sample volumes ranging from 250 µL to 30 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different urine sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin.
These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.
NanoSight® Analysis
Exosomes enriched with Norgen’s Urine Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration.
Exosomal RNA Analysis
Exosomal RNA can be isolated using Norgen’s Exosomal RNA Isolation Kit from exosomes enriched using Norgen’s Urine Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for Biomarker discovery.
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.
Important Note Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.
Staphylococcus Aureus PCR Pathogen Detection Kit For Isothermal Fluorescence Detector
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Product Description
Product Description:
Staphylococcus Aureus PCR Detection Kit for Isothermal Fluorescence Detector
Amp-future Bio’s Pathogen Detection Kit is a revolutionary breakthrough in infectious disease testing. It provides medical professionals with a comprehensive and rapid solution for the diagnosis of infectious diseases. Our Pathogen Detection Kit can detect a wide variety of infectious agents, including bacteria, viruses, parasites and fungi, within 5-20 minutes. It is designed for use with a variety of sample types, including blood, saliva, and other bodily fluids. Our kit features a powerful molecular diagnostic system that is tailored to detect pathogens quickly and accurately. This system is designed to be used by medical professionals and offers superior accuracy and sensitivity. With our Pathogen Detection Kit, medical professionals can quickly diagnose and treat infectious diseases with confidence. Our kit is also designed for easy storage at -20°C, ensuring reliable results.
Amp-future bio, a leading Chinese manufacturer of detection kits, is proud to introduce its new Pathogen Detection Kit. This innovative swab test kit is specially designed for rapid detection of influenza and other infectious diseases, delivering results in as little as 5-20 minutes. Featuring a storage temperature of -20ºC, this test kit is highly reliable and easy to use. It is ideal for hospitals, medical centers, and other healthcare facilities to quickly and accurately detect pathogens. As a trusted name in the industry, Amp-future bio guarantees the highest quality and safety when it comes to its Pathogen Detection Kit, making it the perfect choice for all your rapid test kits and infectious disease test kits needs.
Support and Services:
Pathogen Detection Kit technical support and services are offered to customers to ensure optimal performance and usability of the product. Technical support is available through our customer service hotline as well as our online customer portal. Our technical support team is available to answer any questions and provide guidance for product installation, troubleshooting, and maintenance. Additionally, our team provides software updates and upgrades, as well as access to our library of technical documents and resources. We also offer training and education services for customers who wish to better understand the product and its capabilities.
Packing and Shipping:
To ensure that our Pathogen Detection Kit arrives to you in perfect condition, we take special care in packaging and shipping.
Before shipping, all kits are carefully inspected for product quality. They are then bubble-wrapped and sealed in a box to provide the highest level of protection.
We use a reputable shipping company with tracking options and signature confirmation. Depending on the shipping destination, the package may require additional documentation, such as a Certificate of Origin or an invoice.
We strive to deliver your order as quickly and cost-effectively as possible. We offer same day shipping for orders placed before noon EST.
FAQ:Q1: What is Amp-future bio Pathogen Detection Kit?A1: Amp-future bio Pathogen Detection Kit is a high-performance product developed by China for rapid detection of pathogenic microorganisms.Q2: What microorganisms can be detected by this kit?A2: The Amp-future bio Pathogen Detection Kit can detect a wide range of pathogenic microorganisms, including bacteria, viruses, and parasites.Q3:Is the kit easy to use?A3: Yes. The Amp-future bio Pathogen Detection Kit is designed for easy operation and use. It comes with detailed instructions for use.Q4: Is the kit safe?A4: All components of the Amp-future bio Pathogen Detection Kit are strictly tested to ensure safety. The kit is safe and reliable to use.
Document
Staphylococcus Aureus PCR Detection Kit for Isothermal Fluorescence Detector
Amp-future Bio’s Pathogen Detection Kit is a revolutionary breakthrough in infectious disease testing. It provides medical professionals with a comprehensive and rapid solution for the diagnosis of infectious diseases. Our Pathogen Detection Kit can detect a wide variety of infectious agents, including bacteria, viruses, parasites and fungi, within 5-20 minutes. It is designed for use with a variety of sample types, including blood, saliva, and other bodily fluids. Our kit features a powerful molecular diagnostic system that is tailored to detect pathogens quickly and accurately. This system is designed to be used by medical professionals and offers superior accuracy and sensitivity. With our Pathogen Detection Kit, medical professionals can quickly diagnose and treat infectious diseases with confidence. Our kit is also designed for easy storage at -20°C, ensuring reliable results.
Propane-1,3-(PEG12-DBCO),2-alcohol is a PEG linker containing two DBCO moieties and a terminal primary hydroxyl group. The hydroxyl can react with a variety of functional groups and the hydrophilic PEG spacer arm can provide better solubility to labeled molecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research use only.
Document
Propane-1,3-(PEG12-DBCO),2-alcohol is a PEG linker containing two DBCO moieties and a terminal primary hydroxyl group. The hydroxyl can react with a variety of functional groups and the hydrophilic PEG spacer arm can provide better solubility to labeled molecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research use only.
