DL8M Largest Capacity Refrigerated Centrifuge

Features

1. Widely used in the field of blood bank, pharmaceutical factory and laboratory.

2. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.

3. Digital display which indicates the program, speed, time, RCF, temperature.

4. Micro computer control, there are 10 kinds of program and 10 kinds off acceleration and deceleration for your choice.

5. There are 4 kinds of rotors for your choice.

6. Electric lid lock, compact design, super speed and imbalance protection.

7. The centrifuge body is made of high-quality steel, safe and reliable.

DL8M Technical Parameter：

Max. Speed	8000rpm
Max. RCF	14900×g
Max.   Capacity	8×2000ml(16*500ml blood bag）
Time Range	1~9h59min
RPM/RCF Convert	Yes
Noise (dB)	≤ 65
Temperature	-20~40℃
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	±1℃
Voltage(V/Hz)	AC   220V/380V 50HZ/60HZ
Dimensions   (L x W x Hmm)	940×890×1000mm /
Net   Weight(Kg)	600KG
Certificates	CE,ISO &   Calibration report are available

Matched Rotors for DL8M

Order No.	Rotor No.	Max Speed (rpm)	Max Volume(ml)	Max. RCF（×g）
8M-1	Swing Rotor	4000	8 x 2000ml	5716
8M-2	Swing Rotor	4000	6×2400ml	5330
8M-3	Angle Rotor	8000	6×1000ml	14900
8M-4	Swing Rotor	4200	2×6×1000ml	5680

DL8M large capacity refrigerated centrifuge max capacity can hold 8x2000ml, can for 16pcs 500ml blood bags, can also for 8 bottles of 2000ml

Introduction

The HiPure Minipreps system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Miniprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.

Details

Specifications

Features	Specifications
Main Functions	Isolation up to 30μg endotoxin-free plasmid DNA from 1-5ml bacterial culture using 96-well bind plate
Applications	Cell transfection, animal injection, etc.
Purification method	96 well plate
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Low copy plasmid vector
Sample amount	1-5ml LB（x96）
Yield	30μg
Elution volume	≥75μl
Time per run	≤60 minutes
Liquid carrying volume per column	800μl
Binding yield of column	70μg

Principle

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

1. High throughput: processing 96 samples at once;
2. High yield: The purified plasmid yield can reach up to 30μg, which is greater than the yield of the magnetic bead method kit;
3. Detoxification: endotoxin content <0.1EU/μg;
4. Fast: The entire extraction can be completed in 60 minutes without the need for time-consuming alcohol precipitation;

Kit Contents

Contents	P115701	P115702	P115703
Purification Times	1 x 96 Preps	4 x 96 Preps	20 x 96 Preps
RNase A	10 mg	20 mg	100 mg
Buffer P1	30 ml	120 ml	550 ml
Buffer P2	30 ml	120 ml	550 ml
Buffer LEN3	15 ml	60 ml	300 ml
Buffer LN4	80 ml	270 ml	2 x 700 ml
Buffer LN5	40 ml	220 ml	1100 ml
Buffer PW1	40 ml	220 ml	1100 ml
Buffer PW2	25 ml	100 ml	2 x 200 ml
Elution Buffer	30 ml	60 ml	250 ml
Lysate Clear Plate	1	4	20
HiPure DNA Plate	1	4	20
1.6ml Collection Plate	2	8	40
0.5ml Collection Plate	1	4	20

Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.

Purchase Guide

Name	CAT NO	Features	Sampleamount	Elutionvolume	Yield
HiPure Plasmid Mini Kit	P1001	Classic, Regular application	1-5ml LB	30-50μl	5–35µg/5ml
HiPure Plasmid Mini Kit II	P1002	Classic, Regular application	5-15ml LB	75-100μl	20-80µg/15ml
HiPure Plasmid Plus 96 Kit	P1006	Classic, Regular application	1-1.5ml LB	70-150μl	1-15μg/1ml
HiPure Fastfilter Plasmid Midi Kit	P1013	Fast	30-50ml LB	0.3-0.8ml	50–250µg/50ml
HiPure Fastfilter Plasmid Maxi Kit	P1014	Fast	100-200ml LB	0.5-1.5ml	0.4–1mg/200ml
Low endotoxin/Endotoxin-free Plasmid
HiPure Plasmid EF Maxi Kit	P1156	Classic method, General for all kinds of vectors	100-200ml LB	≥0.7ml	200–1500µg
HiPure Plasmid EF Mini Kit	P1154	Recommend for low copy vector, Thoroughly remove RNA	5-15ml LB	≥60μl	10–80µg
HiPure Plasmid EF 96 Kit	P1157	Recommend for low copy vector, Thoroughly remove RNA	1-5ml LBx96	≥75μl	30µg
HiPure Plasmid EF Midi Kit	P1231	Recommend for High copy vector, Low elution volume, High concentration	25-50ml LB	≥100μl	10-500μg
HiPure Plasmid EF Mega Kit	P1116	Recommend for High copy vector(>3μg/ml)	500ml LB	≥2ml	1-10mg

For any technical problems or customized products, please contact us.

F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here

The HiPure Minipreps system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Miniprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.