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GeneAb™ FSH

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Follicle-Stimulating Hormone (FSH) allows for progression of ovarian folliculogenesis, and enables Sertoli cell proliferation in the testis. Anti-FSH reacts with FSH-producing cells, therefore FSH staining is useful for classifying pituitary cancers and understanding pituitary disease.

Detail

Description

Specifications

CloneIHC580
SourceMouse Monoclonal
Positive ControlPituitary
Dilution Range1:200

Other Products

N-bis(PEG2-propargyl)-N-(PEG2-amidoPEG1)-N-(bis(PEG2-propargyl)

N-bis(PEG2-propargyl)-N-(PEG2-amidoPEG1)-N-(bis(PEG2-propargyl) is a branched PEG linker with four terminal alkyne groups. The alkyne groups can react with azides via copper catalyzed Click Chemistry reactions. The PEG units help increase the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

AAV Purification Kits

Overview

  • AAV Purification from any input – cell fraction or media fraction
  • High AAV recovery, up to 90%
  • No specialized equipment needed
  • Purification from a variety of AAV serotypes (including AAV6 and AAV9)
  • Yields highly active AAV for in vivo and in vitro experiments
  • Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

Recombinant adeno-associated virus (AAV) vectors are highly promising tools for both in vitro and in vivo gene transfer. Norgen’s AAV Purification Kits provide fast and simple procedures for concentrating and purifying AAV vectors from cell lysate and cell culture media. Purification is based on precipitation onto Norgen Biotek’s proprietary resin. Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. AAV vector purified in this manner is highly active for use in in vitro and in vivo transduction experiments.

AAV Purification Kit

Norgen’s AAV Purification Kit contains sufficient materials for 15 preparations (33.5 mL per prep of supernatant (SN) or a total of 500 mL of supernatant input). Approximately 1 mL of cell pellet can be purified per prep, up to a maximum of 15 mL of cell pellet in total for the entire kit. Up to 33X sample concentration.

AAV Purification Mini Kit

Each spin column is able to concentrate and purify AAV from 0.5-8 mL of cell pellet, cell culture media, or cells and culture media mixed together. Up to 50X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (200 µL). Preparation time for 4 samples is 1.5 hours, with 45 minutes of hands-on time.

AAV Purification Midi Kit

Each spin column is able to concentrate and purify AAV from 8 mL up to 45 mL of input consisting of cell pellet, cell culture media, or cells and culture media mixed together. Up to 50X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (1 mL). The kit may be used to purify up to 8 x 25 mL or 4 x 45 mL of samples using the included columns. Preparation time for 4 samples is approximately 2 to 2.5 hours, with 1.5 hours of hands on time.

AAV Purification Maxi Kit (Slurry Format)

Each spin column is able to concentrate and purify AAV from 45 mL to 90 mL of input consisting of cell pellet, cell culture media, or cells and culture media mixed together. Up to 200X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (1-10 mL) using the optional concentration step. The kit may be used to purify up to 1 x 900 mL samples or 10 x 45-90 mL samples using the included columns. Preparation time for 1 x 900 mL sample is approximately 2.5 to 3.5 hours, with an optional concentration step requiring an additional 30 min.

Details

Supporting Data

Figure 1 / 10

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Figure 1
Figure 1
Figure 2
Figure 3. Transduction of HTX cells
Figure 4. Linear Scaling of AAV Purification Mini Kit From 2.5 mL - 15 mL
Figure 5. Transduction of HTX cells
Figure 6. Purification from Both Cell Pellet and Supernatant
Figure 7. Linear Scaling of AAV Purification Midi Kit From 2 mL to 50 mL
Figure 8. Transduction of HTX cells
Figure 9. Cellular Transduction of HTX cells
Figure 10. Elution Titer Across VariousInput Volumes from 40 to 80 mL

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Kit Specifications
Resin Binding Capacity (total per kit)At least 5 x 1010 AAV particles as determined by qPCR
AAV Vector SerotypeAAV6, AAV9 and others
Input TypeCells, media
Input Volume (AAV supernatant)1 – 33.5 mL SN per prep
(500 mL SN in total)
Input Volume (AAV cell pellet)1 mL cell pellet per prep
(15 mL in total)
Time to Complete Purifications2.5 to 4.5 hours with 1 hour hands on time
In vivo transductionYes

Storage Conditions and Product Stability

HL-SAN Nuclease should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

ComponentCat. 66100 (15 preps)Cat. 63200 (20 preps)Cat. 63300 (4-8 preps)Cat. 63250 (1-10 preps)
Lysis Buffer S5.5 mL5.5 mL5.5 mL20 mL
DNAse I2 x 25 uL2 x 25 uL210 μL
RNAse A60 μL60 μL240 μL
HL-SAN Nuclease102 μL
Binding Buffer A20 mL4 mL4 mL2 x 8 mL
Purification Solution C60 mL
Purification Solution D130 mL
Wash Solution C2 x 130 mL60 mL60 mL3 x 60 mL
Slurry E12.5 mL2 x 14.5 mL
Elution Buffer O66 mL8.5 mL8.5 mL66 mL
Protein Neutralizer4 mL4 mL4 mL4 mL
Spin Columns20
Mini Spin Columns20
Midi Spin Columns (grey contents) with Collection Tubes810
Midi Spin Columns (white contents) with Collection Tubes8
Maxi Spin Columns (grey contents) with Collection Tubes10
Maxi Spin Columns (white contents) with Collection Tubes10
Collection Tubes40
Elution tubes (1.7 mL)5020
Midi Elution tubes (15 mL)810
Maxi Elution tubes (50 mL)10
Product Insert1111

HCM188 Tryptose Phosphate Broth

Introduction

Discover our Tryptose Phosphate Broth, formulated to support the cultivation of fastidious microorganisms, including Haemophilus influenzae and Neisseria species. Ideal for microbiological research, pharmaceutical testing, and vaccine production.