GeneAb™ FSH

Opentrons Thermocycler GEN2 Seals are adhesive-backed Ethylene Propylene Diene Monomer (EPDM) seals designed for use with the fully-automated Thermocycler Module GEN2. They come in a package of 10 seals. These seals need to be applied to the hood of the Thermocycler and can be reused up to 20 times. Opentrons Thermocycler GEN2 Seals need to be sterilized before each use.

Note, if you have a Thermocycler Module GEN1, please use the Thermocycler GEN1 Seals.

Opentrons Thermocycler GEN2 Seals are adhesive-backed Ethylene Propylene Diene Monomer (EPDM) seals designed for use with the fully-automated Thermocycler Module GEN2. They come in a package of 10 seals. These seals need to be applied to the hood of the Thermocycler and can be reused up to 20 times. Opentrons Thermocycler GEN2 Seals need to be sterilized before each use.

Note, if you have a Thermocycler Module GEN1, please use the Thermocycler GEN1 Seals.

DBCO-PEG36-TFP ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules. The TFP ester is less susceptible to undergo hydrolysis compared to the NHS ester. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG36-TFP ester is an amine-reactive, labeling reagent used to modify proteins, antibodies, and other amine-containing biopolymers. The hydrophilic PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules. The TFP ester is less susceptible to undergo hydrolysis compared to the NHS ester. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• No need for staining and destaining of denaturing gels since Norgen’s loading buffer contains ethidium bromide
• Highly stable
• Nine bands from 200 bases to 4000 bases
• Convenient lyophilized format provides better product stability

The Norgen 1 kb RNA Ladder is a set of RNA transcripts derived from recombinant DNA templates. This ladder is suitable for precise sizing of a wide range of RNA molecules using native or denaturing agarose gels, and can be visualized under UV.

Contents: 
• 2 vials of lyophilized RNA ladder (25 loads each)
• 1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution
• 2x loading buffer

Details

Supporting Data

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Specifications:
• Nine discrete bands, ranging from 200 to 4,000 bases
• Higher intensity band at 1,000 bases for easy reference

Contents:
• 2 vials of lyophilized RNA ladder (25 loads each)
• 1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution
• 2x loading buffer

Instructions:
To reconstitute the lyophilized RNA ladder, add 250 µL of 1x loading buffer to each 25 loads vial and vortex gently.
Heat at 80°C for 10 minutes. Incubate on ice for 1 min. Load 10 µL on a 1-2% gel. For optimal results, use Norgen 2x loading buffer with each RNA sample. No need for staining and destaining of denaturing gels since Norgen’s loading buffer contains ethidium bromide.

Store at -20°C.

Documentation