The Volcano product family supports this goal with optimized reaction components for sensitive and reliable results. The key driver is an engineered, truly thermostable Taq DNA polymerases with reverse transcriptase activity. Have a look at the products or contact us directly for support optimizing your products:
Detail
We accelerate RT-PCR for SARS-CoV-2 detection and make Covid-19 research easier!
The Volcano product family supports this goal with optimized reaction components for sensitive and reliable results. The key driver is an engineered, truly thermostable Taq DNA polymerases with reverse transcriptase activity. Have a look at the products or contact us directly for support optimizing your products:
Volcano3G® RT-PCR Probe 2x Master Mix Volcano3G® RT-PCR Probe 2x Master Mix contains all components required for general RT-qPCR. (to the product)
RevTaq RT-PCR DNA polymerase RevTaq RT-PCR DNA polymerase has RNA-dependent (reverse transcriptase) and DNA-dependent DNA polymerase activity. It is a thermostable DNA polymerase suitable for RT-PCR. (to the product)
Volcano3G® RT-PCR Probe 2x Master Mix (with SARS-CoV-2 Research Components) If you are interested in sourcing a Volcano3G® RT-PCR Probe 2x Master Mix with components needed for SARS-CoV-2 research, please contact us.
Other Products
Norovirus (NoV) TaqMan RT-PCR Detection Kits
Product Info
Document
Product Info
Overview
Detection kits for NoV
CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis
Norovirus is a single-stranded, non-enveloped RNA virus belonging to the Caliciviridae family. Norovirus is considered the major causative agent of non-bacterial gastroenteritis in the world. The main channel of transmittance is via contaminated food or water, as well as human-to-human contact. Most Noroviruses infecting humans belong to genogroup I and II (particularly genotype 4). Norovirus is very stable in the environment and is resistant to some surface disinfectants such as alcohols and detergents. Moreover, it is very difficult to culture Norovirus in vitro, making its identification by standard microbiological assays challenging.
NoV TaqMan RT-PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
NoV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Propargyl-PEG4-CH2CO2tBu is a PEG reagent that can reacts with azide compounds or biomolecules under the catalyzation of copper. The t-butyl protected carboxyl group can be deprotected under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG4-CH2CO2tBu is a PEG reagent that can reacts with azide compounds or biomolecules under the catalyzation of copper. The t-butyl protected carboxyl group can be deprotected under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
For the rapid detection and enumeration of coliform bacteria.
Principle:
Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; sodium chloride maintains osmotic equilibrium; agar as medium coagulant; dodecyl sulfate inhibit Gram-positive bacteria; chromogenic substrate and large intestine flora β- galactosidase-glucosidase specific reaction, hydrolysis of the substrate, the release of the color groups produce green colonies on the light yellow plate.
Formulation (per liter):
Peptone :10g
Yeast extract powder: 3g
sodium chloride:5g
sodium lauryl sulfate:0.1g
Agar :12g
Chromogenic substrate 2.7g
Final pH 7.0 ± 0.2
How to use:
1. Weigh 32.8g of this product, adding 1L of distilled or deionized water , heated to boiling stirring until completely dissolved, dispensing into flask without autoclaving.
2,Take 25g or 25mL of sample with aseptic procedures, added to the flask containing 225mL of sterile phosphate buffered saline (or saline) is shaken thoroughly homogenized with a homogenizer or a 1:10 dilution 1min, then 1:10 dilution continue to select the appropriate serial dilutions of three, two plates each dilution was inoculated.
3, the use of pour method: The medium is cooled in a water bath to about 50 , sterilized petri dish having a diameter 90mm, 1ml samples were inoculated per dish, then poured into about 15ml of the above dissolution medium, and mix to solidify, upside down, 37 for 24 hours.
4, using surface inoculation: cooled to about 50 , shake devoted to the medium in sterile petri dish. Can be stored in the refrigerator for a day or stored at room temperature for several days (dark, 4 ). The sample was streaked method or filter method, 37 for 24 hours.
5, observe the results.
Quality control:
This product appear light yellow after pouring into plate, these strains were inoculated after 36 ± 1 18 ~ 24h culture growth in the following table.
Growth of bacteria were cultured bacteria numbers feature
Escherichia coli ATCC25922 good green colonies
Citrobacter ATCC8090 good green colonies
Salmonella typhimurium CMCC50115 good colorless colonies
Enterococcus faecalis ATCC29212 suppressed —–
Storage: Store at 10-30 , dark, cool and dry place, tighten the cap immediately after use. Storage period of two years.
